孟俊龙,张明亮,刘靖宇,常明昌,冯翠萍,武晨剑

• 1:山西农业大学食品科学与工程学院
• 2:黄土高原食用菌提质增效协同创新中心

摘要(Abstract)：

本研究以蛹虫草菌丝体为供试材料,采用高效液相色谱对提取的蛹虫草菌丝体水溶性黄色素的组分进行了分离纯化与测定分析,并以其主要组分YP-4为检测指标,采用温度、光照、pH、金属离子、常见食品添加剂等条件对蛹虫草菌丝体水溶性黄色素稳定性的影响进行了较为系统的研究。结果表明,蛹虫草菌丝体水溶性黄色素由5种结构相似的类胡萝卜素组分组成。其中,主要组分YP-4占到WSYPs总量的57.43%,YP-4的MS data [M-H]为521.2657,紫外特征吸收峰为:209nm、239nm、421nm、445nm、474nm,在445nm处有最大吸收峰。以YP-4为检测指标,对提取蛹虫草菌丝体黄色素的稳定性测试结果表明,该类黄色素具有良好的加工稳定性,但在加工过程中应尽量缩减紫外光照射时间,注意酸性添加剂和Fe3+的用量。这一研究结果将为蛹虫草菌丝体水溶性黄色素乃至其他优质天然黄色素的开发利用提供参考依据。

关键词(KeyWords)： 蛹虫草;水溶性黄色素;分离纯化;稳定性

Abstract:

Keywords:

基金项目(Foundation): 山西省自然科学基金面上项目(201801D121240);; 2016年度山西省重点研发计划（重点）项目（201603D21106）;; 山西省煤基重点科技攻关项目(FT2014-03-01)~~

作者(Author): 孟俊龙,张明亮,刘靖宇,常明昌,冯翠萍,武晨剑

DOI: 10.13346/j.mycosystema.190121

参考文献(References)：

• Aberoumand A,2011.A review article on edible pigments properties and sources as natural biocolorants in foodstuff and food industry.World Journal of Dairy and Food Sciences,6(1):71-78
• Chen C,Tolgor B,Bao HY,2013.Chemical composition analysis of cultured Cordyceps militaris.Food Science,34(11):36-40(in Chinese)
• Dai YC,Zhou LW,Yang ZL,Wen HA,Bau T,Li TH,2010.Arevised checklist of edible fungi in China.Mycosystema,29(1):1-21(in Chinese)
• Dong JZ,Wang SH,Ai XR,Yao L,Sun ZW,Lei C,Wang Y,Wang Q,2013.Composition and characterization of cordyxanthins from Cordyceps militaris fruit bodies.Journal of Functional Foods,5(3):1450-1455
• Fu MJ,2005.Study on the carotenoid produced from Cordyceps militaris L.Journal of Food Science and Biotechnology,24(5):107-110(in Chinese)
• George B,2005.Carotenoid chemistry and biochemistry.China Light Industry Press,Beijing.179-185(in Chinese)
• Jian JH,Guo LQ,Ye ZW,Liu C,Yin L,Lin JF,2015.Research progress of Cordyceps militaris novel pigment.China Food Additives,11:137-140(in Chinese)
• Lu RL,Luo FF,Hu FL,Huang B,Li CR,Bao GH,2013.Identification and production of a novel natural pigment,cordycepoid A,from Cordyceps bifusispora.Applied Microbiology and Biotechnology,97:6241-6249
• Ma L,Lin XM,2010.Effects of lutein and zeacarotene on aspects of eye health.Journal of the Science of Food and Agriculture,1:2-12
• Ma ZC,Chen WH,Li HX,2016.Chemistry of natural edible pigment.China Light Industry Press,Beijing.1-198(in Chinese)
• Ranga RA,Raghunath RRL,Baskaran V,Sarada R,Ravishankar GA,2010.Characterization of microalgal carotene by mass spectrometry and their bioavailability and antioxidant properties elucidated in rat model.Journal of Agricultural and Food Chemistry,15:8553-8559
• Sharoni Y,Linnewiel-Hermoni K,Khanin M,Salman H,Veprik A,Danilenko M,Levy J,2012.Carotenoids and apocarotenoids in cellular signaling related to cancer:a review.Molecular Nutrition and Food Research,56:259-269
• Velmurugan P,Kamala-Kannan S,Balachandar V,Lakshmanaperumalsamy P,Chae JC,Oh BT,2010a.Natural pigment extraction from five filamentous fungi for industrial applications and dyeing of leather.Carbohydrate Polymers,79:262-268
• Velmurugan P,Lee YH,Venil CK,Lakshmanaperumalsamy P,Chae JC,Oh BT,2010b.Effect of light on growth,intracellular and extracellular pigment production by five pigment-producing filamentous fungi in synthetic medium.Journal of Bioscience and Bioengineering,109(4):346-350
• Yan XT,Bao HY,Bau T,2010.Isolation and identification of one natural pigment from cultured Cordyceps militaris.Mycosystema,29(5):777-781(in Chinese)
• Yang T,Dong CH,2014.Photo morphogenesis and photo response of the blue-light receptor gene Cmwc-1 in different strains of Cordyceps militaris.FEMSMicrobiology Letters,352(2):190-197
• Zhang ML,Liu JY,Chang MC,Meng JL,Zhao H,Hou XX,2018.Extraction and anti-oxidative activity of yellow pigments from Cordyceps militaris.Mycosystema,37(6):761-771(in Chinese)
• Zhang X,Zhao WE,Hu LQ,Zhao L,Huang JY,2011.Carotene inhibit proliferation and regulate expression of peroxisome proliferators-activated receptor gamma(PPARc)in K562 cancer cells.Archives of Biochemistry and Biophysics,1:96-106
• Zhou ZY,Liu JK,2010.Pigments of fungi(macromycetes).Natural Product Reports,27:1531-1570
• 陈策，图力古尔，包海鹰，2013.人工蛹虫草的化学成分分析.食品科学，34(11):36-40
• 戴玉成，周丽伟，杨祝良，文华安，图力古尔，李泰辉，2010.中国食用菌名录.菌物学报，29(1):1-21
• 付鸣佳，2005.蛹虫草产类胡萝卜素的研究.食品与生物技术学报，24(5):107-110
• 惠伯棣，2005.类胡萝卜素化学及生物化学.北京：中国轻工业出版社.179-185
• 简锦辉，郭丽琼，叶志伟，刘聪，殷林，林俊芳，2015.蛹虫草新型色素的研究进展.中国食品添加剂，2015(11):137-140
• 马自超，陈文田，李海霞，2016.天然食用色素化学.北京：中国轻工业出版社.1-198
• 闫喜涛，包海鹰，图力古尔，2010.人工培养蛹虫草中一种天然色素的分离和结构鉴定.菌物学报，29(5):777-781
• 张明亮，刘靖宇，常明昌，孟俊龙，赵慧，侯笑笑，2018.蛹虫草黄色素提取工艺优化及其体外抗氧化研究.菌物学报，37(6):761-771