内生真菌产紫杉醇研究的回顾与展望Recent research advances on endophytic fungi producing taxol
康冀川;靳瑞;文庭池;何劲;雷邦星;
摘要(Abstract)：
紫杉醇是一种高效、低毒、广谱的天然抗癌药物,是人类未来20年间最有效的抗癌药物之一。近年来的研究发现,从植物内生真菌中发酵生产紫杉醇被证明是解决药源问题的有效途径。本文概述了微生物发酵生产紫杉醇的研究进展,包括产紫杉醇内生真菌的分离、多样性和真菌产紫杉醇的优势,紫杉醇的提取和检测技术,同时对如何提高内生真菌紫杉醇产量进行了比较全面的综述。随着现代分子生物学技术和微生物发酵工程的发展,工业上大规模发酵生产紫杉醇将有望实现。
关键词(KeyWords)： 紫杉醇;内生真菌;生物多样性;发酵工程
基金项目(Foundation): 贵州大学创新基金[红豆杉产紫杉醇及其类似物内生真菌的研究(校研农2010006)]
作者(Authors): 康冀川;靳瑞;文庭池;何劲;雷邦星;
DOI: 10.13346/j.mycosystema.2011.02.001
参考文献(References)：
- Chakravarthi BV,Das P,Surendranath K,Karande AA,Jayabaskaran C,2008.Production of paclitaxel by Fusarium solani isolated from Taxus celebica.Bioscience,33:259-267
- Chen JH,Liu JJ,Zang GG,2004.Screening of taxol-producing endophytic fungi and regulation of fermentation conditions.Journal of Central South University,35(1):65-69(in Chinese)
- Chen YJ,Zhang Z,Wang Y,2003.Screening endophytic fungus to produce taxol from Taxus yunnanensis.Biotechnology,13(2):10-11(in Chinese)
- Cheng L,Ma QM,Tao GJ,Tao WY,Wang RM,Yang J,Guo XL,2007.Systemic identification of a paclitaxel-producing endofungus.Industrial Microbiology,4:23-30(in Chinese)
- Dai WL,2008.Strain improvement of fungus Fusarium Mairei and precursors effector on taxol production.Master Dissertation.Jiang Nan University,Wuxi.12-13,37(in Chinese)
- Dai WL,Tao WY,2008.Preliminary study on fermentation conditions of taxol-producing endophytic fungus.Chemical Industry and Engineering Progress,27:883-886,891
- Do CB,Cormier F,1990.Accumulation of anthocyanins enhanced by a high osmotic potential in grape(Vitis vinifera L.)cell suspensions.Plant Cell Reports,9(3):143-146
- Gangadevi V,Muthumary J,2008.A novel endophytic taxol-producing fungus Chaetomella raphigera isolated from a medicinal plant,Terminalia arjuna.Applied Biochemistry Biotechnology,158(3):675-684
- Gangadevi V,Muthumary J,2009.Taxol production by Pestalotiopsis terminaliae,an endophytic fungus of Terminalia arjuna(arjun tree).Applied Biochemistry Biotechnology,52(1):9-15
- Guo BH,Wang YC,Zhou XW,Hu K,Tan F,Miao ZQ,Tang KX,2006.An endophytic taxol-producing fungus BT2isolated from Taxus chinensis var.mairei.African Journal of Biotechnology,5:875-877
- Holton HA,Somoza C,Kim HB,1994.First total synthesis of taxol.Journal of the American Chemical Society,116(4):1597-1599
- Hu K,Tan F,Tang KX,2006.Isolation and screening of endophytic fungal synthesizing taxol from Taxus chinensis var.mairei.Journal of Southwest China Normal University(Natural Science Edition),31(1):134-137(in Chinese)
- Huang QL,Roessner CA,Croteau R,2001a.Engineering Escherichia coli for the synthesis of taxadiene,a key intermediate in the biosynthesis of taxol.Bioorganic&Medicinal Chemistry Letters,9:2237-2242
- Huang YJ,Wang JF,Li GL,2001b.Antitumor and antifungal activities in endophytic fungi isolated from pharmaceutical plants Taxus mairei,Cephalataxus fortunei and Torreya grandis.FEMS Immumology and Medical Microbiology,31:163-167
- Jennewein S,Park H,DeJong JM,2005.Coexpression in yeast of taxus cytochrome P450reductase with cytochrome P450oxygenases involved in taxol biosynthesis.Biotechnology Bioengineering,5:588-598
- Kumaran RS,Hur BK,2009.Screening of species of the endophytic fungus Phomopsis for the production of the anticancer drug taxol.Applied Biochemistry Biotechnology,54(1):21-30
- Kumaran RS,Muthumary J,Hur BK,2008.Taxol from Phyllosticta citricarpa,a leaf spot fungus of the angiosperm Citrus medica.Journal of Bioscience and Bioengineering,106:103-106
- Kumaran RS,Muthumary J,Kim EK,Hur BK,2009.Production of taxol from Phyllosticta dioscoreae,a leaf spot fungus isolated from Hibiscus rosa-sinensis.Biotechnology and Bioprocess Engineering,14:76-83
- Li C,Yuan YJ,Hu ZD,2001.Studies on relation between production of taxanes Taxus chinesis var.mairei.Journal of Shihezi University,5(2):109-112(in Chinese)
- Li GL,Wang JF,Huang YJ,2001.Screening of endophytic fungi with antitumor activities.Mycosystema,20(3):387-391(in Chinese)
- Li JL,2006.Studies on the taxol-producing of Taxus endophytic fungus.Master Dissertation.Northeast Agriculture University,Harbin.17-20(in Chinese)
- Li JY,Sidhu RS,Bollon A,1998.Stimulation of taxol production in liquid cultures of Pestalotipsis microspora.Mycological Research,102:461-464
- Li JY,Strobel GA,Sidhu R,1996.Endophytic taxol producing fungi from Bald Cypress Taxodium distichum.Microbiology,142:2223-2226
- Lin FC,Liu XH,Wang HK,2003.Recent research and prospect on taxol and its producing fungi.Acta Microbiologica Sinica,43(4):534-538(in Chinese)
- Liu JJ,Gong HX,Yang DL,Chen SJ,Yang L,2006.Study on endophytic fungi producing taxol isolated from Taxus yunnanensis.Progress in Modern Biomedicine,6(12):53-55(in Chinese)
- Liu K,Ding X,Deng B,Chen W,2009.Isolation and characterization of endophytic taxol-producing fungi from Taxus chinensis.Journal Industry Microbiological Biotechnology,36:1171-1177
- Liu XL,Zhou DP,Sun JQ,2002.A study on fermentation process for production of taxol by Nodulisporium sylviforme.Mycosystema,21(2):246-251(in Chinese)
- Ma TY,Dong ZL,1999.The study of isolating endophytic fungus synthesizing taxol from plant.Journal of Northwest University,29(1):47-49(in Chinese)
- Ma YC,Zhao K,Wang SW,Zhang J,Qi XH,Zhou DP,2003.Biological diversity of taxol-producing endophytic fungi.Journal of Fungal Research,1(1):28-32(in Chinese)
- Nicolaou KC,Yang Z,Liu J,1994.Total synthesis of taxol.Nature(Lond.),367:630-634
- Petrini O,Sieber TN,Toti L,Viret O,1992.Ecology,metabolite production,and substrate utilization in endophytic fungi.Natural Toxins,1:185-196
- Qiu DY,Huang MJ,Fang XH,1994.Isolation of an endophytic fungus associated with Taxus yunnanensis.Mycosystema,13(4):314-316(in Chinese)
- Stierle A,Strobel G,Stierle D,1993.Taxol and taxane production by Taxomyces andreanae,an endophytic fungus of Pacific yew.Science,260(9):214-216
- Stierle A,Strobel G,Stierle D,1995.The search for a taxol-producing microorganism among the endophytic fungi of the Pacific yew,Taxus brevifolia.Journal of Natural Products,58(9):1315-1324
- Strobel GA,Hess WM,Li JY,1997.Pestalotiopsis guepinii,a taxol-producing endophyte of the Wollemi pine,Wollemia nobilis.Australian Journal of Botany,45(6):1073-1082
- Strobel GA,Yang XS,Sears J,1996.Taxol from Pestalotiopsis microspora,an endophytic fungus of Taxus wallichiana.Microbiology,142:435-440
- Sun DF,Ran XQ,Wang JF,2008.Isolation and identification of a taxol-producing endophytic fungus from Podocarpus.Acta Microbiologica Sinica,48:589-595(in Chinese)
- Tan F,Hu K,Zhu SQ,Tang KX,Liao ZH,2004.A protocol for extraction of high-yield endophytic fungi producing taxol from Taxus.CN200410081264.0(in Chinese)
- Tian RP,Yang Q,Zhou GL,2006.Taxonomic study on a taxol producing fungus isolated from bark of Taxus chinensis var.mairei.Journal of Wuhan Botanical Research,24(6):541-545(in Chinese)
- Venkatachalam R,Subban K,Paul MJ,2008.Taxol from Botryodiplodia theobromae(BT115):an endophytic fungus of Taxus baccata.Journal of Biotechnology,136(1):189-190
- Wang JF,Lu HY,Su WJ,1999.A taxol-producing endophytic fungus isolated from Taxus mairei and its antitumor activity.Journal of Xiamen University,38(4):485-487(in Chinese)
- Wang SW,Ma X,Ping WX,Zhou DP,2007.Research advances on taxol producion by microbe fermentation.Microbiology,34(3):561-565
- Wang YC,2007.Genetic transformation of taxol-producing endophytic fungi and molecular cloning of genes involved in taxol biosynthesis pathway.PhD dissertation.Shanghai Jiao Tong University,Shanghai.106-107(in Chinese)
- Wani MC,Taylor HL,Wall ME,Coggon P,McPhail AT,1971.Plant antitumor agents.VI.The isolation and structure of taxol,a novel antileukemic and antitumor agent from Taxus brevifolia.Journal of the American Chemical Society,93:2325-2327
- Woo HL,Swenerton KD,Hoskins PJ,1996.Taxol is active in platinum-resistant endometrial adenocarcinoma.Journal of Clinical Oncology,19(3):290-291
- Xiang Y,Cui JX,Lu AG,2002.Culture and fermentation conditions of taxoids-produced endophytic fungi Alternaria sp.1011.Journal of Northeast Forestry University,30(3):17-20(in Chinese)
- Xu F,2006.Strain improvement and optimization of the fermentation condition of taxol-producing fungus Fusarium mairei.Master Dissertation.Jiang Nan University,Wuxi.8-12(in Chinese)
- Yan B,Bi JN,Ji Y,Wang WJ,Zhao Y,Zhu XD,2007.An endophytic taxol-producing fungus from cypress.Acta Scientiarum Naturalium Universitatis Nankaiensis,40:67-70(in Chinese)
- Yang L,Liu JJ,Yang DL,Zhu WH,2007.Screening of endophytic fungi producing baccatin-Шfrom Taxus yunnanensis and preliminary optimization of the culture media.Progress in Modern Biomedicine,7(5):692-695(in Chinese)
- Yu Y,Hu C,2007.Separation and identification of a new Taxus chinensis var.mairei endophytic fungus(Bionectria sp.)and theactivity of its metabolites.Southwest University,29:131-135(in Chinese)
- Yukimune Y,Tabata H,Higashi Y,1996.Methyl jasmonate-induced over production of paclitaxel and baccatin-III in taxus cell suspension cultures.Nature Biotechnology,14(9):1129-1132
- Zamir LO,Nedea ME,Garneau FX,1992.Biosynthetic building blocks of Taxus canadensis var.taxanes.Tetrahedron Letter,33(36):5235-5236
- Zhang P,Zhou PP,Jiang C,Yu H,Yu LJ,2008.Screening of taxol producing fungi based on PCR amplification from Taxus.Biotechnological Letter,30:2119-2123
- Zhang YN,Dong ZL,2002.A study on fermentation condition of endophytic fungus associated with taxol.Northwest University(Natural Science Edition),32(3):310-312(in Chinese)
- Zhao K,Ping W,Li Q,Hao S,Zhao L,Gao T,Zhou D,2009.Aspergillus niger var.taxi,a new variety of taxol producing fungus isolated from Taxus cuspidata in China.Journal of Applied Microbiology,107:1202-1207
- Zhao K,Zhao LF,Wei JY,Zhou DP,2008.Isolation of a taxol-producing endophytic fungus and inhibiting effect of the fungus metabolites on HeLa cell.Mycosystema,27:735-744
- Zhao K,Zhou DP,Wang W,2003.Effects of medium components on taxol production of Nodulisporium sylviforme.Journal of Fungal Research,l(1):24-27(in Chinese)
- Zhou DP,Ping WX,Sun JQ,Zhou XH,Liu XL,Yang DZ,Zhang JP,Zheng XQ,2001a.Isolation of taxol producing fungi.Microbiology,21(1):18-19,32(in Chinese)
- Zhou DP,Sun JQ,Yu HY,Ping WX,Zheng XQ,2001b.Nodulisporium,a genus new to China.Mycosystema,20(2):277-278(in Chinese)
- Zhou XW,Wang ZN,Jiang KJ,Wei YM,Lin J,Sun XF,Tang KX,2007.Screening of taxol-producing endophytic fungi from Taxus chinensis var.mairei.Applied Biochemistry and Microbiology,43(4):439-443
- Zhu JX,Li YC,Meng L,2008.Comparative study on different parts of taxol-producing endophytic fungi from Taxus chinensis in Taihang Mountain.Biotechnology Bulletin,4:191-194(in Chinese)
- Zhu XX,Liu GM,1997.The relationship on the yield of taxol and ecological environment from Taxus.Chinese Traditional and Herbal Drugs,1997(A10):17-20(in Chinese)
- 陈建华,刘佳佳,臧巩固,2004.紫杉醇产生菌的筛选与发酵条件的调控.中南大学学报(自然科学版),35(1):65-69
- 陈毅坚,张灼,王艳,2003.云南红豆杉内生真菌中产紫杉醇真菌的筛选.生物技术,13(2):10-11
- 程龙,马奇明,陶冠军,陶文沂,王荣明,杨静,过晓磊,2007.一株产紫杉醇的丝状真菌的系统分类鉴定.工业微生物,4:23-30
- 代文亮,2008.产紫杉醇菌种的改良及若干种前体物质作用的研究.无锡:江南大学硕士学位论文.12-13,37
- 胡凯,谈锋,唐克轩,2006.南方红豆杉中产紫杉醇内生真菌的分离和筛选.西南师范大学学报(自然科学版),3l(1):134-137
- 李春,元英进,胡宗定,2001.南方红豆杉悬浮培养细胞中糖代谢的波动性及其与紫杉烷合成关系的研究.石河子大学学报(自然科学版),5(2):109-112
- 李桂玲,王建锋,黄耀坚,2001.植物内生真菌抗肿瘤活性菌株的筛选.菌物系统,20(3):387-391
- 李嘉琳,2006.产紫杉醇的红豆杉内生真菌研究.哈尔滨:东北农业大学硕士学位论文.17-20
- 林福呈,刘小红,王洪凯,2003.紫杉醇及其产生菌的研究现状与展望微生物学报,43(4):534-538
- 刘佳佳,龚汉祥,杨栋梁,陈淑娟,杨磊,2006.产紫杉醇类云南红豆杉内生真菌筛选的研究.现代生物医学进展,6(12):53-55
- 刘晓兰,周东坡,孙剑秋,2002.树状多节孢发酵生产紫杉醇工艺条件的初步研究.菌物系统,21(2):246-251
- 马天有,董兆麟,1999.从植物分离产紫杉醇的内生真菌的研究.西北大学学报,29(1):47-49
- 马玉超,赵凯,王世伟,张建,齐晓辉,周东坡,2003.产紫杉醇内生真菌的生物多样性.菌物研究,1(1):28-32
- 邱德有,黄美娟,方晓华,1994.一种云南红豆杉内生真菌的分离.菌物系统,13(4):314-316
- 孙端方,冉雪琴,王嘉福,2008.一株产紫杉醇罗汉松内生真菌的分离和鉴定.微生物学报,48:589-595
- 谈锋,胡凯,祝顺琴,唐克轩,廖志华,2004.一种提高红豆杉内生真菌发酵物中紫杉醇产率的方法.专利号:CN200410081264.0
- 田仁鹏,杨桥,周国玲,2006.一株产紫杉醇的南方红豆杉内生真菌的分类研究.武汉植物学研究,24(6):541-545
- 汪业春,2007.产紫杉醇内生真菌的遗传转化及紫杉醇合成途径相关基因的克隆.上海:上海交通大学博士学位论文.106-107
- 王建锋,吕华鹰,苏文金,1999.一株新的紫杉醇产生菌及其抗肿瘤活性.厦门大学学报,38(4):485-487
- 王世伟,马玺,平文祥,周东坡,2007.微生物发酵生产紫杉醇研究进展.微生物学通报,34(3):561-565
- 项勇,崔京霞,吕安国,2002.产紫杉烷类物质的链格孢1011菌种的培养和发酵条件.东北林业大学学报,30(3):17-20
- 徐峰,2006.产紫杉醇内生真菌Fusarium mairei的菌种改良和发酵优化研究.无锡:江南大学硕士学位论文.8-12
- 严冰,毕建男,纪元,王文婕,赵阳,朱旭东,2007.一株柏树内生真菌产生抗肿瘤药物紫杉醇.南开大学学报(自然科学版),40:67-70
- 杨磊,刘佳佳,杨栋梁,朱婉华,2007.红豆杉内生真菌中巴卡亭Ⅲ产生菌的筛选及培养基的初步优化.现代生物医学进展,7(5):692-695
- 余艳,胡昌华,2007.南方红豆杉内生真菌Bionectria sp.的分离、鉴定及代谢产物活性研究.西南大学学报,29:131-135
- 张亚妮,董兆鳞,2002.一株产紫杉醇真菌发酵条件的研究.西北大学学报(自然科学版),32(3):310-312
- 赵凯,周东坡,王伟,2003.培养基组成对树状多节孢紫杉醇产量的影响.菌物研究,1(1):24-27
- 周东坡,平文祥,孙剑秋,周晓辉,刘晓兰,杨德柱,张建平,2001a.紫杉醇产生菌分离的研究.微生物学杂志,21(1):18-19,32
- 周东坡,孙剑秋,于寒颖,平文祥,郑喜群,2001b.中国一新记录属多节孢属.菌物系统,20(2):277-278
- 竺俊鑫,李勇超,孟丽,2008.红豆杉中产紫杉醇内生真菌分离部位的比较研究.生物技术通报,4:191-194
- 朱旭祥,刘光明,1997.红豆杉中紫杉醇含量与生态环境的关系.中草药,1997(A10):17-20