昆虫肠道木霉及其对芒果炭疽病菌拮抗作用Trichoderma spp. from insect guts and their antagonistic effects against mango anthracnose
任森;胡伊慧;张漫漫;高圣风;侯巨梅;刘铜;
摘要(Abstract)：
为获得优良生防木霉菌株,本研究以昆虫肠道为样本,从中分离鉴定木霉菌株,并以芒果炭疽病菌盘长孢状刺盘孢为靶标菌,通过对峙培养、挥发性物质和非挥发性物质筛选拮抗效果最优的木霉菌株,测定其孢子悬浮液对芒果炭疽病的室内防效研究。结果显示,从105份昆虫肠道中共分离获得10株木霉,通过形态学特征和Tef1-Rpb2双基因联合建树,鉴定出长枝木霉、哈茨木霉和棘孢木霉各3株,加纳木霉1株;通过平板对峙培养显示加纳木霉HNDF-T-6对芒果炭疽病菌抑菌效果最好,其抑菌率为85.64%;其挥发性物质和非挥发性物质对菌丝生长抑制率分别为38.42%和44.01%。通过室内防效测定,经加纳木霉HNDF-T-6孢子悬浮液处理后的叶片病斑直径减少46.04%,表明该菌株对芒果炭疽病具有较好的生防潜能。
关键词(KeyWords)： 昆虫肠道;芒果炭疽病;加纳木霉;防效
基金项目(Foundation): 海南省热带香料饮料作物工程技术研究中心开放课题(2019xys001);; 国家自然科学基金(31760510)~~
作者(Authors): 任森;胡伊慧;张漫漫;高圣风;侯巨梅;刘铜;
DOI: 10.13346/j.mycosystema.210184
参考文献(References)：
- Ahmad M,Pataczek L,Hilger TH,Zahir ZA,Hussain A,Rasche F,Schafleitner R,Solberg S?,2018.Perspectives of microbial inoculation for sustainable development and environmental management.Frontiers in Microbiology,9:2992
- Alvindia DG,2018.The antagonistic action of Trichoderma harzianum strain DGA01 against anthracnose-causing pathogen in mango cv.‘Carabao’.Biocontrol Science&Technology,28(6):591-602
- Bell DK,1982.In vitro antagonism of Trichoderma species against six fungal plant pathogens.Phytopathology,72(4):279-382
- Benítez T,Rincón AM,Limón MC,Codón AC,2004.Biocontrol mechanisms of Trichoderma strains.International Microbiology,7(4):249-260
- Chen SH,Li M,Jiang XL,Yi T,Zhang NJ,Wang H,Bai LL,2016.Selection of Trichoderma spp.antagonistic to Cylindrocarpon destructans,pathogen of ginseng root rot disease.Chinese Journal of Biological Control,32(2):265-269 (in Chinese)
- Dennis C,Webster J,1971.Antagonistic properties of species-groups of Trichoderma:II.Production of volatile antibiotics.Transactions of the British Mycological Society,57(1):41-48
- Ding CW,Feng Q,Li CH,2020.Inhibition mechanism of Bacillus megaterium LB01 against Colletotrichum gloeosporioides in postharvest mangos.Science and Technology of Food Industry,41(10):131-137 (in Chinese)
- Dou K,Gao J,Zhang C,Yang H,Jiang X,Li J,Li Y,Wang W,Xian H,Li S,Liu Y,Hu J,Chen J,2019.Trichoderma biodiversity in major ecological systems of China.Journal of Microbiology,57(8):668-675
- Engel P,Moran NA,2013.The gut microbiota of insectsdiversity in structure and function.FEMS Microbiology Reviews,37(5):699-735
- Feng WH,Lun JX,Jiang Y,Yu X,2012.Antibacterial properties of Trichoderma viride on Colletotichum gloeosporioides Penz.of mango.Journal of Chinese Institute of Food Science and Technology,12(1):99-104(in Chinese)
- Gams W,Bissett J,1998.Morphology and identification of Trichoderma.In:Kubicek CP,Harman GE (eds.)Trichoderma and Gliocladium.Vol.1.Taylor&Francis,London.3-34
- Jang S,Kikuchi Y,2020.Impact of the insect gut microbiota on ecology,evolution,and industry.Current Opinion in Insect Science,41:33-39
- Jiang Y,Wang JL,Chen J,Mao LJ,Feng XX,Zhang CL,Lin FC,2016.Trichoderma biodiversity of agricultural fields in East China reveals a gradient distribution of species.PLo S One,11(8):e0160613
- Jin P,Wang H,Tan Z,Xuan Z,Dahar GY,Li QX,Miao W,Liu W,2020.Antifungal mechanism of bacillomycin Dfrom Bacillus velezensis HN-2 against Colletotrichum gloeosporioides Penz.Pesticide Biochemistry and Physiology,163:102-107
- Li QL,Bu JY,Tang LH,Huang SP,Guo TX,Mo JY,2020.Advances in mango anthracnose (Colletotrichum gloeosporioides).Journal of Microbiology,40(1):117-124 (in Chinese)
- Li XJ,Li CJ,Yao CX,Song RF,Liu C,Qiu R,Chen YG,Bai JK,Li SJ,2020.Screening,identification of antagonistic Trichoderma spp.against tobacco black shank and its growth promotion effect on tobacco.Chinese Tobacco Science,41(3):65-70 (in Chinese)
- Liu SY,2013.Effect of Trichoderma viride fermentation broth on inhibition against Colletotrichum gloeosporioides and preservation of mango fruit.Master Thesis,Jimei University,Xiamen.14-20 (in Chinese)
- Mwangi MW,Monda EO,Okoth SA,Jefwa JM,2011.Inoculation of tomato seedlings with Trichoderma harzianum and arbuscular mycorrhizal fungi and their effect on growth and control of wilt in tomato seedlings.Brazilian Journal of Microbiology,42(2):508-513
- Oyedokun AV,Adeniyi DO,2016.Microbial diversity in the gut of cashew stem girdler,Analeptes trifasciata fabricius (Coleoptera:Cerambycidae),in Ibadan,Nigeria.International Journal of Insect Science,8:17-22
- Rajaofera MJN,Wang Y,Dahar GY,Jin P,Fan L,Xu L,Liu W,Miao W,2019.Volatile organic compounds of Bacillus atrophaeus HAB-5 inhibit the growth of Colletotrichum gloeosporioides.Pesticide Biochemistry and Physiology,156:170-176
- Samuels GJ,Hebbar PK,2015.Trichoderma:identification and agricultural applications.American Phytopathological Society Press,St.Paul.1-196
- Sergio SV,Guzmán-Ortiz DA,Gómez-Lim MA,Délano-Frier JP,De-Folter S,Sánchez-García P,Pe?a-Cabriales JJ,2013.Potential use of Trichoderma asperellum T8a as a biological control agent against anthracnose in mango.Biological Control,64(1):37-44
- Sun H,Yang LR,Quan X,Xue BG,Zhu ZX,Wu C,2011.Research advances on mechanism of biological control and application about Trichoderma spp.Chinese Agricultural Science Bulletin,27(3):242-246 (in Chinese)
- Tarayre C,Bauwens J,Brasseur C,Mattéotti C,Millet C,Guiot PA,Destain J,Vandenbol M,Portetelle D,de Pauw E,Haubruge E,Francis F,Thonart P,2015.Isolation and cultivation of xylanolytic and cellulolytic Sarocladium kiliense and Trichoderma virens from the gut of the termite Reticulitermes santonensis.Environmental Science and Pollution Research International,22(6):4369-4382
- Tian YQ,Li W,Jiang ZT,Jing MM,Shao YZ,2017.The preservation effect of Metschnikowia pulcherrima yeast on anthracnose of postharvest mango fruits and the possible mechanism.Food Science and Biotechnology,27(1):95-105
- Vinale F,Sivasithamparam K,Ghisalberti EL,Marra R,Lorito M,2008.Trichoderma-plant-pathogen interactions.Soil Biology and Biochemistry,40(1):1-10
- Wei YX,Zhang H,Liu XM,Liu YN,Zhang KL,Zhou FX,Pu JJ,2016.Correlation between laccase lac1 and pathogenicity of Colletotrichum gloeosporioides causing mango anthracnose.China Plant Protection,36(11):5-10(in Chinese)
- Xia XF,Zheng DD,Lin HL,You MS,2013.Isolation and identification of bacteria from the larval midgut of the diamondback moth,Plutella xylostella.Chinese Journal of Applied Entomology,50(3):770-776 (in Chinese)
- Xu CQ,2013.Screening,identification of a antagonistic yeast against mango anthracnose and evaluation of its protectants.Master Thesis,Huazhong Agricultural University,Wuhan.32-34 (in Chinese)
- Yang PY,Li P,Ren BY,Li CG,2019.Analysis on environmental costs of crop pest chemical management technologies in China.China Plant Protection,39(6):27-30+43 (in Chinese)
- Zhang S,Sun F,Liu L,Bao L,Fang W,Yin C,Zhang Y,2020.Dragonfly-associated Trichoderma harzianum QTYC77is not only a potential biological control agent of Fusarium oxysporum f.sp.cucumerinum but also a source of new antibacterial agents.Journal of Agricultural and Food Chemistry,Doi:10.1021/acs.jafc.0c05760
- Zhu H,Wang ZX,Fan MZ,Luo XM,2005.Selection of Trichoderma strains for suppression of strawberry mold disease caused by Botrytis cinerea.Chinese Journal of Biological Control,2005(1):52-54 (in Chinese)
- 陈书华，李梅，蒋细良，衣涛，张念洁，汪浩，白龙律，2016.防治人参锈腐病木霉菌的筛选及防治效果.中国生物防治学报，32(2):265-269
- 丁从文，冯群，李春焕，2020.巨大芽孢杆菌LB01对采后芒果炭疽病菌的抑制机制初探.食品工业科技，41(10):131-137
- 冯卫华，伦家欣，蒋雨，于新，2012.绿色木霉菌抑制芒果炭疽菌特性研究.中国食品学报，12(1):99-104
- 李其利，卜俊燕，唐利华，黄穗萍，郭堂勋，莫贱友，2020.芒果炭疽菌研究进展.微生物学杂志，40(1):117-124
- 李小杰，李成军，姚晨虓，宋瑞芳，刘畅，邱睿，陈玉国，白静科，李淑君，2020.拮抗烟草疫霉菌的木霉菌株筛选鉴定及防病促生作用研究.中国烟草科学，41(3):65-70
- 刘淑宇，2013.绿色木霉菌发酵液对芒果炭疽菌抑制作用及采后保鲜效果研究.集美大学硕士论文，厦门.14-20
- 孙虎，杨丽荣，全鑫，薛保国，朱自贤，武超，2011.木霉生防机制及应用的研究进展.中国农学通报，27(3):242-246
- 韦运谢，张贺，刘晓妹，刘娅楠，张恺莉，周芳雪，蒲金基，2016.芒果胶孢炭疽菌漆酶lac1与致病力的相关性分析.中国植保导刊，36(11):5-10
- 夏晓峰，郑丹丹，林海兰，尤民生，2013.小菜蛾幼虫中肠细菌的分离鉴定.应用昆虫学报，50(3):770-776
- 许春青，2013.芒果炭疽病菌拮抗酵母的筛选、鉴定及其保护剂的评价.华中农业大学硕士论文，武汉.32-34
- 杨普云，李萍，任彬元，李春广，2019.我国农作物病虫害化学防控技术的环境成本分析.中国植保导刊，39(6):27-30+43
- 朱虹，汪章勋，樊美珍，骆绪美，2005.草莓灰霉病拮抗木霉菌株筛选及温室防效测定.中国生物防治，2005(1):52-54