宋自力;廖头根;张伟;陈国参;李伟;汪世华;尹文兵;

• 1:福建农林大学生命科学学院福建省病原真菌与真菌毒素重点实验室
• 2:中国科学院微生物研究所真菌学国家重点实验室
• 3:云南中烟工业有限责任公司技术中心
• 4:河南省科学院生物研究所

摘要(Abstract)：

烟梗是烟草工业的重要副产物,也是宝贵的自然资源。本研究首先利用白腐菌漆酶对烟梗丝进行预处理,提升了添加烟梗丝的卷烟品质;然后分别以木质素、纤维素、半纤维素和果胶的降解率为响应值,采用Box-Behnken设计建立方程模型,对漆酶、纤维素酶、半纤维素酶和果胶酶组成的复合酶预处理烟梗丝条件进行了优化。结果表明:每100g烟梗丝加入30U漆酶,在料液比为35%、温度为30℃、酶解pH为5处理48h的条件下预处理的烟梗丝对提升卷烟品吸效果最佳,烟梗丝中木质素、纤维素、半纤维素和果胶的降解率分别为20.16%、15.10%、7.20%和12.40%;为获得与之相同的各组分降解率,响应面法优化漆酶复合酶最佳处理条件为:每100g烟梗丝加入漆酶14.72U、纤维素酶1.00U、半纤维素酶1.00U、果胶酶8.45U。验证发现烟梗丝各组分降解率实测值与理论值无显著性差异,且显微结构观察显示复合酶处理后的烟梗丝表面致密结构被破坏,孔洞数量明显增加。本研究获得的白腐菌漆酶预处理后的烟梗丝在卷烟中的添加能有效改善卷烟品质,且漆酶复合酶的使用大幅减少了漆酶的用量,降低了漆酶预处理烟梗丝的成本,为废弃烟梗生物质的资源化利用提供了重要依据。

关键词(KeyWords)： 漆酶;复合酶;响应面法;烟梗;资源化利用

Abstract:

Keywords:

基金项目(Foundation): 云南中烟工业有限责任公司科技重大专项(2017CP01)~~

作者(Authors): 宋自力;廖头根;张伟;陈国参;李伟;汪世华;尹文兵;

DOI: 10.13346/j.mycosystema.190396

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