樊香萍;张姝;张永杰;

• 1:山西大学生命科学学院
• 2:农业农村部农业微生物资源收集与保藏重点实验室

摘要(Abstract)：

虫草在世界范围内广泛分布，具有多种生物活性及较高的食药用价值。为进一步丰富虫草真菌资源，本研究对从广东韶关丹霞山采集到的一份野生虫草样品进行了菌株的分离鉴定、生物活性评价和子实体的诱导培养。通过形态学和系统发育分析，将菌株ZYJ0835鉴定为布莱克韦尔虫草Cordycepsblackwelliae。利用5种不同的培养基进行液体发酵培养，发现该菌株的发酵上清液和菌丝体均具有抗氧化和纤溶活性。此外，该菌株还具有一定的抑菌活性。利用大米、小麦、柞蚕蛹3种培养基质，均成功诱导出子实体，并且人工培养的子实体也具有抗氧化和纤溶活性。本研究首次在中国境内报道布莱克韦尔虫草的分布，为进一步开发利用该虫草资源提供了理论依据。

关键词(KeyWords)： 布莱克韦尔虫草;抗氧化;纤溶;抑菌;子实体

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31872162);; 农业农村部农业微生物资源收集与保藏重点实验室开放基金项目(KLMRCP 2021-04)~~

作者(Authors): 樊香萍;张姝;张永杰;

DOI: 10.13346/j.mycosystema.220284

参考文献(References)：

• Bai WF,Guo LQ,Lin JF,Kang LZ,Zeng HR,2013.Screening and fermentation of fibrinolytic enzymeproducing strains from Cordyceps militaris.Modern Food Science and Technology,29(5):1014-1018 (in Chinese)
• Chen B,Sun Y,Luo F,Wang C,2020.Bioactive metabolites and potential mycotoxins produced by Cordyceps fungi:a review of safety.Toxins,12(6):410
• Chen WH,Liang JD,Han YF,Zou X,Zhang YJ,Liang ZQ,2021.Research overviews of Cordyceps:past,present and future.Mycosystema,40(11):2894-2905 (in Chinese)
• Chen WH,Liu C,Han YF,Liang JD,Tian WY,Liang ZQ,2020.Two new recorded species in Cordyceps sensu lato.Microbiology China,47(3):710-717 (in Chinese)
• Dong C,Guo S,Wang W,Liu X,2015.Cordyceps industry in China.Mycology,6(2):121-129
• Duan DE,2019.Taxonomy and phylogeny of Cordycipitaceae from Vietnam.Master Thesis,Yunnan University,Kunming.1-88 (in Chinese)
• Fu L,Chen ZH,2004.Research advances of chemical constituents and pharmacological activities of Cordyceps.Life Science Research,8(1):1-7 (in Chinese)
• Han YF,Zhang YW,Dong X,Huang Y,Liang ZQ,2012.Studies on antibacterial activities of fermentation broth and mycelia extraction of Cordyceps militaris.Hubei Agricultural Sciences,51(16):3492-3495 (in Chinese)
• Hu FL,Li ZZ,2007.Secondary metabolites and their bioactivities of Cordyceps and its related fungi.Mycosystema,26(4):607-632 (in Chinese)
• Hyde KD,Norphanphoun C,Maharachchikumbura SSN,et al.(more than 20 authors),2020.Refined families of Sordariomycetes.Mycosphere,11(1):305-1059
• Isaka M,Kittakoop P,Kirtikara K,Hywel-Jones NL,Thebtaranonth Y,2005.Bioactive substances from insect pathogenic fungi.Accounts of Chemical Research,38(10):813-823
• Li CR,Nam SH,Geng DG,Fan MZ,Li ZZ,2006.Artificial culture of seventeen Cordyceps spp.Mycosystema,25(4):639-645 (in Chinese)
• Liu CY,Li L,Hao XY,Yuan Y,Wu XP,Fu JS,2020.Protective effects of polysaccharides from post-harvest waste substrate of Cordyceps militaris MF27 on oxidative damage to liver cells.Mycosystema,39(2):409-420 (in Chinese)
• Liu XL,Zhang WS,Zheng XQ,Shen Y,Sun Y,2012.Isolation and purification of novel fibrinolytic enzymes obtained from fermentation of Cordyceps militaris.Journal of South China University of Technology(Natural Science Edition),40(5):107-114 (in Chinese)
• Mongkolsamrit S,Noisripoom W,Thanakitpipattana D,Wutikhun T,Spatafora JW,Luangsa-ard J,2018.Disentangling cryptic species with isaria-like morphs in Cordycipitaceae.Mycologia,110(1):230-257
• Ren LY,Zhang S,Zhang YJ,2021.Comparative mitogenomics of fungal species in Stachybotryaceae provides evolutionary insights into Hypocreales.International Journal of Molecular Sciences,22(24):13341
• Wang D,Li NX,Liu GY,Dong SR,Chai HX,2014.The extraction and antioxidation activity of polysaccharide from a new strain of Cordyceps militaris.Edible Fungi of China,33(6):56-58 (in Chinese)
• Wang X,Li X,Chen D,2011.Evaluation of antioxidant activity of isoferulic acid in vitro.Natural Product Communications,6(9):1285-1288
• Wu F,Zhou LW,Yang ZL,Bau T,Li TH,Dai YC,2019.Resource diversity of Chinese macrofungi:edible,medicinal and poisonous species.Fungal Diversity,98:1-76
• Xiao JH,Li Y,Xiao Y,Zhong JJ,2013.Advance and prospect of studies on bioactivity and mechanism of Cordyceps fungi.China Journal of Chinese Materia Medica,38(5):640-647 (in Chinese)
• Yang S,Yang X,Zhang H,2020.Extracellular polysaccharide biosynthesis in Cordyceps.Critical Reviews in Microbiology,46(4):359-380
• Yuan Y,Qi M,Zhang FP,Liu Z,Liu K,Wu XP,Zhang JL,Fu JS,2021.Evaluation of the biological activities of four species of Cordyceps (Isaria,Beauveria) mycelial crude polysaccharides.Mycosystema,40(6):1446-1457
• Zeng HB,Li TH,Song B,Lin QY,Huang H,2009.Study on antioxidant activity of Cordyceps guangdongensis.Natural Product Research and Development,21(5):201-204 (in Chinese)
• Zhang S,Zhang YJ,Bhushan S,Xu JP,Wang CS,Liu XZ,2013.Ophiocordyceps sinensis and Cordyceps militaris:research advances,issues and perspectives.Mycosystema,32(4):577-597 (in Chinese)
• Zhang S,Zhang YJ,Liu XZ,Zhang H,Liu DS,2013.On the reliability of DNA sequences of Ophiocordyceps sinensis in public databases.Journal of Industrial Microbiology and Biotechnology,40:365-378
• 白伟芳，郭丽琼，林俊芳，康林芝，曾厚儒，2013.蛹虫草产纤溶酶活性菌株的筛选及产酶条件的研究.现代食品科技，29(5):1014-1018
• 陈万浩，梁建东，韩燕峰，邹晓，张永军，梁宗琦，2021.纵观虫草(真菌)的来世今生.菌物学报，40(11):2894-2905
• 陈万浩，刘畅，韩燕峰，梁建东，田维毅，梁宗琦，2020.广义虫草属二新记录种.微生物学通报，47(3):710-717
• 段东娥，2019.越南虫草科分类及系统发育研究.云南大学硕士论文，昆明.1-88
• 傅岚，陈作红，2004.虫草属真菌化学成分及药理作用研究进展.生命科学研究，8(1):1-7
• 韩燕峰，张延威，董旋，黄玉，梁宗琦，2012.蛹虫草菌株发酵液和菌丝提取物的抑菌活性初探.湖北农业科学，51(16):3492-3495
• 胡丰林，李增智，2007.虫草及相关真菌的次生代谢产物及其活性.菌物学报，26(4):607-632
• 李春如，南圣姬，耿德贵，樊美珍，李增智，2006.十七种虫草的子实体培育研究.菌物学报，25(4):639-645
• 刘城移，李琳，郝心怡，袁源，吴小平，傅俊生，2020.蛹虫草MF27菌糠多糖对肝细胞氧化损伤的保护作用.菌物学报，39(2):409-420
• 刘晓兰，张雯舒，郑喜群，沈媛，孙莹，2012.蛹虫草发酵产物新纤溶酶的分离纯化.华南理工大学学报(自然科学版)，40(5):107-114
• 王多，李宁馨，刘桂宇，董素蓉，柴惠霞，2014.蛹虫草新菌株3号多糖提取及其抗氧化作用.中国食用菌，33(6):56-58
• 肖建辉，李彦，肖瑜，钟建江，2013.虫草属真菌的生物活性及机制研究现状与展望.中国中药杂志，38(5):640-647
• 袁源，戚梦，张凤培，刘钊，刘昆，吴小平，张君丽，傅俊生，2021.4种虫草相关真菌菌丝体粗多糖的生物活性评价.菌物学报，40(6):1446-1457
• 曾宏彬，李泰辉，宋斌，林群英，黄浩，2009.广东虫草抗氧化活性的研究.天然产物研究与开发，21(5):201-204
• 张姝，张永杰，Bhushan S，徐建平，王成树，刘杏忠，2013.冬虫夏草菌和蛹虫草菌的研究现状、问题及展望.菌物学报，32(4):577-597