艾叶,谢泰祥,刘江枫,兰思仁,彭东辉,张清华

• 1:福建农林大学园林学院
• 2:福州市于山风景区管理处
• 3:福建农林大学林学院

摘要(Abstract)：

为研究竹叶兰Arundina graminifolia根系共生真菌的群落结构及对寄主的营养作用,采用高通量测序技术对野生竹叶兰根围土壤、根表、根内3个生态位真菌的种类及生物学功能进行鉴定与预测。所测竹叶兰根系共生真菌的平均辛普森指数为根围0.972、根表0.905、根内0.703,说明3个位点都具有丰富的共生真菌;根围土壤真菌香农指数7.393显著高于根表和根内香农指数4.728和2.872,根表真菌多样性显著高于根内(P<0.05)。根表、根内有子囊菌门、担子菌门、罗兹壶菌门、壶菌门、毛霉菌门和被孢霉门,较根围土壤缺少球囊菌门和梳霉菌门;分子方差分析(AMOVA)证实3个生态位的真菌群落结构差异极显著(P=0.004)。各生态位丰度最高的20个真菌仅属于子囊菌和担子菌的40个属,其中子囊菌有30个属,种类数量占优势,而担子菌的单个种类丰度高于子囊菌。根围土壤腐生型真菌占优势(44.07%);根表主要有腐生、病原、共生型真菌;根内真菌营养类型差异大,样品M1的共生型真菌为优势类型(97.21%),M2、M3以腐生型占优(90.96%)。优势共生型真菌有革菌属Threlephora、根须腹菌属Rhizopogon和Dentiscutata,腐生型真菌主要有长毛孔菌属Funalia和Vuilleminia,均为担子菌。研究结果为揭示竹叶兰与根系真菌的营养关系以及共生真菌的开发提供了依据。

关键词(KeyWords)： 竹叶兰;根共生真菌;群落结构;生物学功能

Abstract:

Keywords:

基金项目(Foundation): 福建省自然科学基金（2016J01703）;; 福建省种业创新与产业化工程项目（ZYCX-LY-2017005）;; 福建农林大学创新项目(KFA17331A)~~

作者(Author): 艾叶,谢泰祥,刘江枫,兰思仁,彭东辉,张清华

DOI: 10.13346/j.mycosystema.190205

参考文献(References)：

• Balabanova LA,Bakunina IY,Slepchenko LV,Kirichuk NN,Khudyakova YV,Son OM,Pivkin MV,Rasskazov VA,2018.Polysaccharide-degrading activity in marine and terrestrial strains of mycelial fungi.Russian Journal of Bioorganic Chemistry,44(4):431-437
• Cameron DD,Leake JR,Read DJ,2006.Mutualistic mycorrhiza in orchids:evidence from plant-fungus carbon and nitrogen transfers in the green-leaved terrestrial orchid Goodyera repens.New Phytologist,171(2):405-416
• Chen RR,Lin XG,Shi YQ,2003.Research advances of orchid mycorrhizae.Chinese Journal of Applied and Environmental Biology,9(1):97-101(in Chinese)
• Dearnaley JW,Martos F,Selosse MA,2012.Orchid mycorrhizas:molecular ecology,physiology,evolution and conservation aspects.In:Hock B(ed.)Fungal associations.Vol.9.Springer,Berlin.207-230
• Edwards J,Johnson C,Santos-Medellin C,Lurie E,Podishetty NK,Bhatnagar S,Eisen JA,Sundaresan V.Structure,variation,and assembly of the root-associated microbiomes of rice.PNAS,112(8):911-920
• Edgar RC,2013.UPARSE:highly accurate OTU sequences from microbial amplicon reads.Nature methods,10:996-998
• Gai XG,Xing XK,Guo SX,2014.Ecological research of orchid mycorrhizae:a review.Mycosystema,33(4):753-767(in Chinese)
• Haas BJ,Gevers D,Earl AM,Feldgarden M,Ward DV,Giannoukos G,Ciulla D,Tabbaa D,Highlander SK,Sodergren E,MethéB,DeSantis TZ,Petrosino JF,Knight R,Birren BW,2011.Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons.Genome Research,21:494-504
• Hu QF,Zhou B,Ye YQ,Jiang ZY,Huang XZ,Li YK,Du G,Yang GY,Gao XM,2013.Cytotoxic deoxybenzoins and diphenylethylenes from Arundina graminifolia.Journal of Natural Products,76(10):1854-1859
• Huang SR,Shen Z,Yang GS,2015.A preliminary study on domestication and cultivation of Arundina graminifolia tube seedlings.Chinese Journal of Tropical Agriculture,35(7):19-21(in Chinese)
• Hynson NA,Madsen TP,Selosse MA,Adam IKU,Ogura-Tsujita Y,Roy M,Gebauer G,2013.The physiological ecology of mycoheterotrophy.In:Merckx VSFT(ed.)Mycoheterotrophy-the biology of plants living on fungi.Springer,New York.297-342
• Liu RJ,Wang L,2018.Biological symbiotics.China Science Publishing&Media Ltd,Beijing.8-9,83-98,255-267(in Chinese)
• Miyamoto Y,Maximov TC,Sugimoto A,2019.Discovery of rhizopogon associated with Larix from northeastern Siberia:insights into host shift of ectomycorrhizal fungi.Mycoscience,https://doi.org/10.1016/j.myc.2019.03.003
• Nguyen NH,Song Z,Bates ST,Branco S,Tedersoo L,Menke J,Schilling JS,Kennedy PG,2016.FUNGuild:an open annotation tool for parsing fungal community datasets by ecological guild.Fungal Ecology,20(1):241-248
• Nucci M,Anaissie Ej,2009.Hyalohyphomycosis.In:Anaissie EJ,McGinnis MR,Pfaller MA(eds.)Clinical mycology.Vol.2.Churchill Livingstone,New York.308-309
• Pandey D,Kehri HK,Zoomi I,Akhtar O,Singh AK,2019.Mycorrhizal fungi:biodiversity,ecological significance,and industrial applications.In:Ajar NY,Shashank M,Sangram S,Arti SG(eds.)Recent advancement in white biotechnology through fungi.Springer Cham,New York.181-199
• Pant B,Shah S,Shrestha R,Pandey S,Joshi PR,2017.An overview on orchid endophytes.In:Varma A,Prasad R,Tuteja N(eds.)Mycorrhiza-nutrient uptake,biocontrol,ecorestoration.Springer Cham,New York.503-524
• Predebon MJ,Bond DR,Brzozowski J,Jankowski H,Deane F,Tarleton M,Shaw AA,Mc Cluskey A,Bowyer MC,Weidenhofer J,Scarlett CJ,2019.The bispidinone derivative3,7-bis-[2-(S)-amino-3-(1H-indol-3-yl)-propionyl]-1,5-dip henyl-3,7-diazabicyclo[3.3.1]nonan-9-one dihydrochloride induces an apoptosis-mediated cytotoxic effect on pancreatic cancer cells in vitro.Molecules,24(3):524
• Rasmussen HN,Dixon KW,JersákováJ,Tě?itelováT,2015.Germination and seedling establishment in orchids:a complex of requirements.Annals of Botany,116(3):391-402
• Rognes T,Flouri T,Nichols B,Quince C,MahéF,2016.VSEARCH:a versatile open source tool for metagenomics.Peer J Preprints,4:e2584
• Sheng JM,Wu XQ,Hou LL,2014.Isolating and identifying mycorrhiza helper bacteria from the rhizosphere soil of Pinus thunbergi inoculated with Rhizipogen luteous.Journal of Northeast Forestry University,42(5):110-114(in Chinese)
• Song XY,Tang BX,Qiu JZ,Liu MF,2018.Isolation and identification of endophytes from Arundina graminifolia and their antioxidant activity.Modern Food Science and Technology,34(2):82-88(in Chinese)
• Tang DY,Wang YJ,Li RY,Wang YQ,2005.Preliminary report on introduction and cultivation of Arundina graminifolia.Journal of Chinese Medicinal Materials,28(4):263-264(in Chinese)
• Wang YS,Liu RJ,2017.A checklist of arbuscular mycorrhizal fungi in the recent taxonomic system of Glomeromycota.Mycosystema,36(7):820-850(in Chinese)
• Warton DI,Wright ST,Wang Y,2012.Distance-based multivariate analyses confound location and dispersion effects.Methods in Ecology and Evolution,3(1):89-101
• Waud M,Busschaert P,Lievens B,Jacquemyn H,2016.Specificity and localised distribution of mycorrhizal fungi in the soil may contribute to co-existence of orchid species.Fungal Ecology,20:155-165
• Wu JR,Han SF,Zhu YY,Lv M,Wang GP,Guo WL,2006.Study on taxonomy of endophytic fungi isolated from orchid mycorrhizae in Yunnan Province.Journal of Southwest Forestry College,26(3):5-10(in Chinese)
• Yang JW,Ling H,Zhang Y,Zeng X,Guo SX,2018.Effects of endophytic fungi on seed germination of medicinal plants of Orchidaceae:a review.Mycosystema,37(1):22-34(in Chinese)
• Zelmer CD,Cuthbertson L,Currah RS,1996.Fungi associated with terrestrial orchid mycorrhizas,seeds and protocormsm.Mycoscience,37(4):439-448
• Zhu ZX,Zhuang WY,2014.Current understanding of the genus Trichoderma(Hypocreales,Ascomycota).Mycosystema,33(6):1136-1153(in Chinese)
• 陈瑞蕊，林先贵，施亚琴，2003.兰科菌根的研究进展.应用与环境生物学报，9(1):97-101
• 盖雪鸽，邢晓科，郭顺星，2014.兰科菌根的生态学研究进展.菌物学报，33(4):753-767
• 黄素荣，谌振，杨光穗，2015.竹叶兰试管苗驯化栽培技术初报.热带农业科学，35(7):19-21
• 刘润进，王琳，2018.生物共生学.北京：科学出版社.8-9,83-98,255-267
• 盛江梅，吴小芹，侯亮亮，2014.黑松-黄色须腹菌共生体根围菌根辅助细菌的筛选及鉴定.东北林业大学学报，42(5):110-114
• 宋新月，汤冰雪，邱君志，刘美凤，2018.竹叶兰内生真菌的分离鉴定及其抗氧化活性研究.现代食品科技，34(2):82-88
• 唐德英，王云娇，李荣英，王云强，2005.野生竹叶兰引种栽培初报.中药材，28(4):263-264
• 王幼珊，刘润进，2017.球囊菌门丛枝菌根真菌最新分类系统菌种名录.菌物学报，36(7):820-850
• 伍建榕，韩素芬，朱有勇，吕梅，王光平，郭文林，2006.云南兰科植物菌根内生真菌种类研究.西南林学院学报，26(3):5-10
• 杨建文，凌鸿，张盈，曾旭，郭顺星，2018.内生真菌对兰科药用植物种子萌发作用研究进展.菌物学报，37(1):22-34
• 朱兆香，庄文颖，2014.木霉属研究概况.菌物学报，33(6):1136-1153