张旭升;郭鹏杰;张瀛澜;郭东罡;张全喜;

• 1:山西大学环境与资源学院
• 2:山西省黄河实验室

摘要(Abstract)：

本研究利用IlluminaMiSeq高通量测序技术，分析安太堡露天煤矿生态复垦区4种植被恢复模式(刺槐Robinia pseudoacacia、油松Pinus tabulaeformis、沙棘Hippophae rhamnoides和柠条锦鸡儿Caraganakorshinskii)土壤真菌群落组成及多样性，并探究其与土壤环境因子的相关性。高通量测序结果共获得821508条有效序列和1841个OTUs，子囊菌门Ascomycota和担子菌门Basidiomycota为优势菌门，序列数占真菌总数的88.43%，此外还含有3.72%未被分类学鉴定的真菌。真菌α多样性分析结果表明：刺槐模式、沙棘模式和柠条锦鸡儿模式的Sobs指数、Chao1指数、Ace指数和Shannon指数均显著高于油松模式(P<0.01)，而油松模式的Simpson指数显著高于其余3种植被恢复模式(P<0.05)。真菌β多样性分析结果显示：OTU水平下4种植被恢复模式土壤真菌群落组成差异显著(R=0.709 9,P=0.005)，其中，油松模式的土壤真菌群落与其他3种模式距离较远，差异显著(P<0.01)，而其余3种植被恢复模式之间两两组成相近，差异不显著(P>0.05)。通过土壤理化性质的测定、典范对应分析、冗余分析以及Spearman相关性分析，结果发现土壤有机质、全氮和有效磷是显著影响4种植被恢复模式土壤真菌群落组成的环境因子。综上所述，在矿区生态复垦过程中刺槐模式是物种相对多度最高的植被恢复模式，油松模式是优势菌群含量最高的植被恢复模式。研究结果可为植被修复煤矿复垦区土壤及真菌资源的开发利用提供理论依据。

关键词(KeyWords)： 煤矿复垦区;高通量测序;真菌资源开发;群落组成

Abstract:

Keywords:

基金项目(Foundation): 山西省回国留学人员科研资助项目(2020-010)~~

作者(Authors): 张旭升;郭鹏杰;张瀛澜;郭东罡;张全喜;

DOI: 10.13346/j.mycosystema.220062

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