冯煊;张小雪;刘猛;刘月涵;张惠;魏欢;杨志新;

• 1:河北农业大学资源与环境科学学院河北省农田生态环境重点实验室

摘要(Abstract)：

本试验采用室内摇瓶培养,通过添加不同浓度的Cu(Ⅱ),研究了Cu(Ⅱ)对降解菌Fusarium sp. ZH-H2形态发育及木质素酶系活性的影响,以期诱导提高木质素酶系的活性。结果表明,当Cu(Ⅱ)浓度为0.50mmol/L时,菌丝生物量最高达0.38g,菌丝团呈洁白平滑球状且数量最多,此浓度下木质素过氧化物酶(ligninperoxidase,LiP)活性峰值也达到最高,为28315.41U/L,当Cu(Ⅱ)浓度为2.00mmol/L时,有利于诱导锰过氧化物酶(manganese peroxidase,MnP)和漆酶(laccase,Lac)的代谢,最高峰值分别为1 612.90U/L、7 829.12U/L,并且明显缩短了峰值的形成时间,浓度过高或过低则对3种木质素酶系活性产生抑制效应,为下一步分子生物学研究奠定了基础。

关键词(KeyWords)： Cu(Ⅱ);PAHs降解菌;形态发育;木质素酶

Abstract:

Keywords:

基金项目(Foundation): 河北省人力资源和社会保障厅项目(C2013003022);; 河北省教育厅项目(Z2013058)~~

作者(Authors): 冯煊;张小雪;刘猛;刘月涵;张惠;魏欢;杨志新;

DOI: 10.13346/j.mycosystema.190351

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