基于稳定同位素比率及营养差异的双孢蘑菇不同栽培基质的溯源分析Origin traceability analyses of different cultivation substrate of Agaricus bisporus based on stable isotope ratio and nutrient difference
宋婷婷;聂晶;袁玉伟;沈颖越;范丽军;蔡为明;
摘要(Abstract):
双孢蘑菇是世界消费量最大的食用菌之一,不同于欧美国家的麦草栽培配方,我国双孢蘑菇栽培材料主要是以水稻秸秆为主。为了降低农业秸秆废弃物的焚烧和我国双孢蘑菇产业的持续发展,需因地制宜地开发和利用各地特色农业废弃物资源作为双孢蘑菇栽培的基质。为鉴别不同材料栽培的双孢蘑菇子实体的营养和产量差异,本研究以8种不同基质配方栽培的双孢蘑菇为对象,比较分析其营养成分,并测定稳定同位素的比例,结果显示,8个培养料配方的粗纤维、钙、锌以及氨基酸含量等4个指标有差异显著。进一步对4个轻质稳定同位素比率的研究结果显示,δ(13)C、δ(13)C、δ(15)N、δ(15)N、δ2H和δ2H和δ(18)O的比率在8个培养基质配方中差异均显著。对筛选到的4个差异显著的营养因子与4个同位素比率共同进行PCA主成分分析的结果表明,可以将不同培养基质配方栽培的双孢蘑菇有效地分为3类,3个主成分的贡献率为78.2%,较好地解释了该分组。通过对稳定同位素和子实体营养指标的分析,获得未来可能应用于双孢蘑菇产业的分级溯源手段。
关键词(KeyWords): 双孢蘑菇;农业秸秆;营养分析;稳定同位素比率
基金项目(Foundation): 浙江省农业新品种选育重大科技专项(2021C02073);; 国家现代农业产业技术体系(CARS-20)~~
作者(Authors): 宋婷婷;聂晶;袁玉伟;沈颖越;范丽军;蔡为明;
DOI: 10.13346/j.mycosystema.210141
参考文献(References):
- Bowen GJ,Wassenaar LI,Hobson KA,2005.Global application of stable hydrogen and oxygen isotopes to wildlife forensics.Oecologia,143:337-348
- Bricout J,Koziet J,1987.Control of the authenticity of orange juice by isotope analysis.Journal of Agricultural and Food Chemistry,35:758-760
- Cengiz MM,Tosun M,Topal M,2018.Determination of the physicochemical properties and13C/12C isotope ratios of some honeys from the northeast Anatolia region of Turkey.Journal of Food Composition and Analysis,69:39-44
- Chai MQ,Li Q,Han PY,Zhao Y,Li YX,Zhang SF,Yao YS,2014.Influence of cultivation medium on yield and nutrient composition of the first tide mushroom.Chinese Agricultural Science Bulletin,33(15):76-79 (in Chinese)
- Chen Y,Chefetz B,Rosario R,Heemst JDH,Romaine CP,Hatcher PG,2000.Chemical nature and composition of compost during mushroom growth.Compost Science Utilization,8(4):347-359
- Choong YK,Xu CH,Lan J,Chen XD,Jamal JA,2014.Identification of geographical origin of lignosus samples using fourier transform infrared and two dimensional infrared correlation spectroscopy.Journal of Molecular Structure,1069(Suppl.C):188-195
- Chung IM,Han JG,Kong WS,Kim JK,An MJ,Lee JH,An YG,Jungd MY,Kim SH,2018.Regional discrimination of Agaricus bisporus mushroom using the natural stable isotope ratios.Food Chemistry,1264:92-100
- Dong H,Xiao KJ,Xian YP,2017.Isotope ratio mass spectrometry coupled to element analyzer and liquid chromatography to identify commercial honeys of various botanical types.Food Analytical Methods,10(8):2755-2763
- Flores P,Hellin P,Fenoll J,2007.The feasibility of usingδ15N andδ13C value for discriminating between conventionally and organically fertilized pepper(Capsicum annuum L.).Journal of Agricultrual Food Chemistry,55(14):5740-5745
- Gong JG,2011.The cultivation technology of straw-decay mushroom in China.Chemical Industry Press,Beijing.106-124 (in Chinese)
- Heaton K,Kelly SD,Hoogewerff J,Woolfe M,2008.Verifying the geographical origin of beef:The application of multi-element isotope and trace element analysis.Food Chemistry,107:506-515
- Jing P,Zhao SJ,Lu MM,Cai Z,Pang J,Song LH,2014.Multiple-fingerprint analysis for investigating quality control of Flammulina velutipes fruiting body polysaccharides.Journal of Agricultural and Food Chemistry,62:12128-12133
- Kaffrnik S,Schir DM,Lehnert K,Baars T,Vetter W,2014.δ13C values and phytanic acid diastereomer ratios:combined evaluation of two markers suggested for authentication of organic milk and dairy products.European Food Research and Technology,238(5):819-827
- Li Y,Zhang J,Li T,Liu H,Wang Y,2016.A comprehensive and comparative study of Wolfiporia extensa cultivation regions by fourier transform infrared spectroscopy and ultra-fast liquid chromatography.PLo S One,11:e0168998
- Li Y,Zhang J,Zhao Y,Li Z,Li T,Wang Y,2014.Characteristic fingerprint based on low polar constituents for discrimination of wolfiporia extensa according to geographical origin using UV spectroscopy and chemometrics methods.Journal of Analytical Methods in Chemistry,9
- Lu LX,2014.Study on screening of new cultivation substrates and nutritional physiology of Agaricus bisporus.Master Thesis,Jilin Agriculture University,Changchun.1-55 (in Chinese)
- Mc Gee CF,2018.Microbial ecology of the Agaricus bisporus mushroom cropping process.Appllied and Microbiology and Biotechnology,102(3):1075-1083
- Ogrinc N,Bat K,Kosir IJ,OSIR IJ,Golob T,Kokkinofta R,2009.Characterization of commercial Slovenian and cypriot fruit juices using stable isotopes.Journal of Agricultrual Food Chemistry,57(15):6764-6769
- Patyshakuliyeva A,Post H,Zhou M,Jurak E,Heck AJR,Hildén KS,Kabel MA,M?kel?MR,Altelaar MAF,Vries RPD,2015.Uncovering the abilities of Agaricus bisporus to degrade plant biomass throughout its life cycle.Environmental Microbiology,17(8):3098-3109
- Qin GJ,Wang X,Chen QJ,Zhang GQ,2017.Changes of lignocellulolytic enzymes and material components in different compost formulas during the production of Agaricus bisporus.Chinese Journal of Applied Environment Biology,23(6):1035-1041 (in Chinese)
- Rummel S,Hoelzl S,Horn P,Rossmann A,Schlicht C,2010.The combination of stable isotope abundance ratios of H,C,N and S with 87Sr/86Sr for geographical origin assignment of orange juices.Food Chemistry,118:890-900
- Scampicchio M,Mimmo T,Capici C,Huck C,Innocente N,Drusch S,Cesco S,2012.Identification of milk origin and process-induced changes in milk by stable isotope ratio mass spectrometry.Journal of Agricultural and Food Chemistry,60(45):11268-11273
- Song TT,Cai WM,Feng WL,Jin QL,Fan LJ,Shen YY,Fang H,and Tian FF,2014.The comparison of microbial communities and histological changes in phase I rice straw-based Agaricus bisporus compost by two different composting method.Scientia Horticulturae,174:95-104
- Song TT,Shen YY,Jin QL,Feng WL,Fan LJ,Cao GT,Cai WM,2021.Bacterial community diversity,lignocellulose components,and histological changes in composting using agricultural straws for Agaricus bisporus production.Peer J,93(3):e10452
- Sonnenberg ASM,Baars JJP,Hendrix PM,Lavrijssen B,Gao W,Weijn A,Mes JJ,2011.Breeding and strain protection in the button mushroom Agaricus bisporus.In:Savoie JM,Foulongne-Oriol M,Largeteau M,Barroso G(eds.) Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products,Genomics,Genetics and Breeding,7:9
- Straatsma G,Gerrits JPG,Thissen JTNM,Amsing JGM,Loeffen H,van Griensven LJLD,2000.Adjusment of the composting process for mushroom cultivation based on initial substrate composition.Bioresource Technology,72:67-74
- Thomas F,Jamin E,2009.2H-NMR and13C-IRMS analyses of acetic acid from vinegar,18O-IRMS analysis of water in vinegar:international collaborative study report.Analytica Chimica Acta,649(1):98-105
- van Griensven LJLD,van Roestel AJJ,2004.The cultivation of the button mushroom Agaricus bisporus in The Netherlands:a successful industry.Revista Mexicana Micologia,19:95-102
- Yama K,Stone BA,Macauley BJ,1994.Compositional changes in compost during composting and growth of Agaricus bisporus.Applied and Environmental Microbiology,60(5):1538-1546
- Zhang HL,Wei JK,Wang QH,Yang R,Gao XJ,Sang YX,Cai PP,Zhang GQ,Chen QJ,2019.Lignocellulose utilization and bacterial communities of millet straw based mushroom(Agaricus bisporus) production.Scientific Reports,https://doi.org/10.1038/s41598-018-37681-6
- 柴美清,李青,韩鹏远,赵毅,李云霞,张锁峰,姚宇升,2017.不同栽培基质对香菇头潮菇产量及子实体营养成分的影响.中国农学通报,33(15):76-79
- 弓建国,2011.食用菌栽培技术.北京:化学工业出版社.106-124
- 鲁丽鑫,2014.双孢蘑菇新基质的筛选及营养生理的研究.吉林农业大学硕士论文,长春.1-55
- 秦改娟,王晓,陈青君,张国庆,2017.不同配方培养料生产双孢蘑菇过程中主要木质纤维素降解酶及物料组分的变化.应用与环境生物学报,23(6):1035-1041