林辉;李娜娜;李佳欢;孙淑静;张燎原;胡开辉;

• 1:福建农林大学生命科学学院
• 2:福建农林大学(古田)菌业研究院

摘要(Abstract)：

本研究收集了19株不同来源的斑玉蕈品种，通过ITS测序构建其系统发育树，随后根据菌株在木屑培养基与葡萄糖培养基的生长速度对菌株进行分类，选取生长速度差异明显的快、中和慢8株斑玉蕈菌株进行出菇实验及胞内外碳代谢指标的测定，探讨斑玉蕈生产性能与胞内外碳代谢的相关性。研究发现收集到的19株菌株均为斑玉蕈，亲缘关系近，遗传分化程度低；选取出的8株斑玉蕈菌株鲜菇产量与菌丝生长速度呈极显著正相关，菌丝生长越快，出菇产量越高。同时，斑玉蕈菌株鲜菇产量与菌丝湿重、还原糖、可溶性蛋白、滤纸酶(FPase)、CMC-Na酶(CMCase)、木聚糖酶和淀粉酶7个胞外碳代谢指标(ECMI)及胞内葡萄糖、己糖激酶(HK)、丙酮酸激酶(Pyk)、柠檬酸合酶(CS)、α-酮戊二酸脱氢酶(KGDH)和葡萄糖-6-磷酸脱氢酶(G6PD)6个胞内碳代谢指标(ICMI)呈显著正相关。说明菌丝生长速度快的斑玉蕈品种，胞外基质分解速度更快，提高可吸收碳源的供应，胞内加强对碳的吸收与同化，为菌丝的增殖提供更多原料与能量。本研究分析了斑玉蕈生产性能与胞内外碳代谢的相关性，为斑玉蕈优良品种的鉴别与选育奠定基础。

关键词(KeyWords)： 斑玉蕈;生产性能;胞内碳代谢指标;胞外碳代谢指标;相关性分析

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(31870481);; 福建省自然科学基金重点项目(2019J02007);; 2020–2022福建省现代食用菌产业技术体系建设(闽农综[144]号)~~

作者(Authors): 林辉;李娜娜;李佳欢;孙淑静;张燎原;胡开辉;

DOI: 10.13346/j.mycosystema.210481

参考文献(References)：

• Andre C,Froehlich JE,Moll MR,Benning C,2007.Aheteromeric plastidic pyruvate kinase complex involved in seed oil biosynthesis in Arabidopsis.The Plant Cell,19(6):2006-2022
• Brown JP,Perham RN,1976.Selective inactivation of the transacylase components of the 2-oxo acid dehydrogenase multienzyme complexes of Escherichia coli.The Biochemical Journal,155(2):419-427
• Cook C,Francocci F,Cervone F,Bellincampi D,Bolwell PG,Ferrari S,Devoto A,2015.Combination of pretreatment with white rot fungi and modification of primary and secondary cell walls improves saccharification.Bio Energy Research,8(1):175-186
• Gibon Y,Blaesing OE,Hannemann J,Carillo P,H?hne M,Hendriks JHM,Palacios N,Cross J,Selbig J,Stitt M,2004.A robot-based platform to measure multiple enzyme activities in Arabidopsis using a set of cycling assays:comparison of changes of enzyme activities and transcript levels during diurnal cycles and in prolonged darkness.The Plant Cell,16(12):3304-3325
• Jia DH,Wang B,Peng WH,Guo Y,Gan BC,Huang ZQ,Zheng LY,2009.Analysis of mycelial growth rate and sclerotia yield of 8 Poria cocos strains.Edible Fungi,31(6):23-24 (in Chinese)
• Langdon RG,1966.Glucose-6-phosphate dehydrogenase from erythrocytes.Methods in Enzymology,9:126-131
• Li MH,Yang YQ,Yang M,Zhou EX,2010.Effects of jing gang mycin on cell wall degrading enzyme activity and soluble proteins of Rhizoctonia solani Kühn.Journal of Huazhong Agricultural University,29(3):272-276 (in Chinese)
• Li SM,Li M,Tian JH,Li WP,2014.Effects of carbon and nitrogen nutrient on mycelial growth and extracellar enzyme activity in Pleurotus geesteranus.Northern Horticulture,2014(2):143-145 (in Chinese)
• Li ZC,Yang SS,1997.Guidelines for production and consumption of edible and medicinal fungi.China Agriculture Press,Beijing.1-416 (in Chinese)
• Lin FX,Cheng SM,Li AZ,Xu XF,Lin F,2006.Correlation and principal component analysis for major quantitative traits in Lentinula edodes.Mycosystema,25(4):579-586(in Chinese)
• Lin H,Zhang SR,Li JH,Jin WS,Sun SJ,Hu KH,2018.Effect of continuous subculture on the productivity of Hypsizygus marmoreus.Journal of Henan Agricultural Sciences,47(5):89-93 (in Chinese)
• Lin XW,Xu WH,2016.Hexokinase is a key regulator of energy metabolism and ROS activity in insect lifespan extension.Aging,8(2):245-258
• Liu DR,Tian F,Wang XF,Wang JM,Qi YC,Song AD,Shen JW,Qiu LY,2016.Construction of high laccase producing strain of Pleurotus ostreatus by promoter swapping.Mycosystema,35(5):597-604 (in Chinese)
• Liu MG,Feng ZY,Huo GH,Gao JH,2008.Breeding of Hypsizygus marmoreus superior hybrid strains H11.Edible Fungi,30(2):17-19 (in Chinese)
• Liu PH,Xie BG,Deng YJ,Jiang YJ,2013.Cloning,structural analyses and expression levels of phosphofructokinase gene in different strains of Volvariella volvacea.Mycosystema,32(2):253-260 (in Chinese)
• Natalia P,Oksana M,Tatyana T,Andrei T,Anatoly N,Irina D,2015.Effect of light wavelengths and coherence on growth,enzymes activity,and melanin accumulation of liquid-cultured Inonotus obliquus (Ach.:Pers.) Pilát.Applied Biochemistry&Biotechnology,176(2):333-343
• Ni XJ,Li J,Chu Y,Zhao YL,Yi Q,2010.Correlations of mycelial growth rates and respiration consumption,extracellular enzyme activities.Edible Fungi of China,29(6):47-48 (in Chinese)
• Ooyen JV,Noack S,Bott M,Reth A,Eggeling L,2012.Improved-lysine production with Corynebacterium glutamicum and systemic insight into citrate synthase flux and activity.Biotechnology and Bioengineering,109(8):2070-2081
• Qin JZ,Shuai B,2008.Relationship of extracellular enzyme with the Phellinus igniarius fruit body.Food and Fermentation Industries,34(7):107-110 (in Chinese)
• Qin KX,Shi YG,Sun BQ,Chen H,He GQ,2016.Comparison crushing methods of Ganoderma lucidum mycelium.Science and Technology of Food Industry,37(9):146-149 (in Chinese)
• Ramírez CM,Mnoz BO,2016.Agroindustrial waste cellulose using fermented broth of white rot fungi.Revista Mexicana de Ingeniería Química,15(1):23-31
• Sastre-Ahuatzi M,Téllez-Téllez M,Díaz-Godínez G,Montiel-González AM,Díaz R,Sánchez C,2007.Mycelial growth of strains of Pleurotus ostreatus developed on agar and its correlation with the productivity in pilot production farm.Brazilian Journal of Microbiology,38(3):568-572
• Sun SJ,Shan SK,Li ZF,Guo YY,Chen WX,Hu KH,2015.Effects of trace elements on the mycelial growth and enzyme activities of Hypsizygus marmoreus.Journal of Fujian Agriculture and Forestry University (Natural Science Edition),44(6):639-645 (in Chinese)
• Sun W,Chang JM,Zhang BL,Han L,Wang Y,2013.Mechanisms of Bacillus subtilis B26-10 against sapstain fungi.Journal of Northeast Forestry University,41(4):120-125 (in Chinese)
• Tao YX,Zhang L,Guo L,Chen RL,Lian LD,Xie BG,2015.Cloning and expression analysis of glucose-6-phosphate dehydrogenase gene and alternative splicing variant in Volvariella volvacea.Mycosystema,34(4):724-733 (in Chinese)
• Wang ML,Zhao Y,Chen MJ,Wang H,2014.Expression analysis of xyn and activity determination of xylanase in different Volvariella volvacea strains.Mycosystema,33(5):1074-1083 (in Chinese)
• Wu F,Zhou LW,Yang ZL,Bau T,Li TH,Dai YC,2019.Resource diversity of Chinese macrofungi:edible,medicinal and poisonous species.Fungal Diversity,98:1-76
• Xie YB,Li DW,Li YF,Zhi CH,2015.The colorimetric method of iodine solution to determine activity ofα-amylase inhibitor.Jiangsu Agricultural Sciences,43(1):301-303 (in Chinese)
• Xin T,Chen H,Chen YQ,Chen W,Garre C,Song YD,Ratledge C,2015.Comparison of biochemical activities between high and low lipid-producing strains of Mucor circinelloides:an explanation for the high oleaginicity of strain WJ11.PLo S One,10(6):e0128396
• Xu JZ,Hu KH,Sun SJ,Zhang JL,Xiao YM,Lin YH,2011.The comparison and analysis of the producing ability of the ligninolytic enzyme system in different types of edible fungi.Acta Agriculturae Universitatis Jiangxiensis,33(2):375-380 (in Chinese)
• Zhang JC,Han ZH,Zhang PQ,Kong XH,Ma QF,Dai XD,Liu JN,2013.Study on the determination method of mycelia growth of Auricularia auriculata in culture substrate.Edible Fungi,5:64-65 (in Chinese)
• Zhang JJ,Chen MJ,Feng ZY,Huang JC,Song XX,Wang H,Chen H,2016.Effects of aromatic compounds on biomass,laccase activities and transcript levels of Hypsizygus marmoreus.Mycosystema,35(9):1130-1138(in Chinese)
• Zhang M,Li H,Liu N,Zhang JJ,Xiao QM,Sun LP,2013.Correlation analysis between mycelial growth rate and fruiting body yield of Pholiota nameko.Liaoning Agricultural Sciences,4:75-76 (in Chinese)
• Zhao FY,Lin JF,Zeng XL,Guo LQ,Wang YH,You LR,2010.Improvement in fruiting body yield by introduction of the Ampullaria crossean multi-functional cellulase gene into Volvariella volvacea.Bioresource Technology,101(16):6482-6486
• 贾定洪，王波，彭卫红，郭勇，甘炳成，黄忠乾，郑林用，2009.8个茯苓菌株的菌丝生长速度及菌核产量分析.食用菌，31(6):23-24
• 李明海，杨迎青，杨媚，周而勋，2010.井冈霉素对水稻纹枯病菌细胞壁降解酶活性和可溶性蛋白的影响.华中农业大学学报(自然科学版)，29(3):272-276
• 李守勉，李明，田景花，李伟平，2014.不同碳、氮源营养对秀珍菇菌丝体生长及其胞外酶活性的影响.北方园艺，2014(2):143-145
• 李志超，杨珊珊，1997.食药用菌生产与消费指南.北京:中国农业出版社.1-416
• 林范学，程水明，李安政，徐学锋，林芳，2006.香菇数量性状的相关性分析和主成分分析.菌物学报，25(4):579-586
• 林辉，詹森然，李佳欢，金文松，孙淑静，胡开辉，2018.斑玉蕈菌种连续传代对其生产性能的影响.河南农业科学，47(5):89-93
• 刘冬忍，田芳，王小飞，王景冒，戚元成，宋安东，申进文，邱立友，2016.启动子替代构建糙皮侧耳漆酶高产菌株.菌物学报，35(5):597-604
• 刘明广，冯志勇，霍光华，高君辉，2008.真姬菇杂交菌株H11的选育.食用菌，30(2):17-19
• 刘朋虎，谢宝贵，邓优锦，江玉姬，2013.草菇磷酸果糖激酶(PFK)基因克隆、结构及不同菌株中表达量分析.菌物学报，32(2):253-260
• 倪新江，李洁，初洋，赵艳丽，尹茜，2010.菌丝生长速度与呼吸消耗及胞外酶活性的关系.中国食用菌，29(6):47-48
• 秦俊哲，帅斌，2008.不同胞外酶与桑黄子实体形成关系的研究.食品与发酵工业，34(7):107-110
• 秦可欣，石彦国，孙冰沁，陈浩，何国庆，2016.灵芝菌丝体破碎处理方法的比较.食品工业科技，37(9):146-149
• 孙淑静，单书凯，李钊锋，郭艳艳，陈文星，胡开辉，2015.不同微量元素对斑玉蕈菌丝生长及酶活的影响.福建农林大学学报(自然科学版)，44(6):639-645
• 孙薇，常建民，张柏林，韩丽，王雨，2013.枯草芽孢杆菌B26-10防治木材蓝变的机理.东北林业大学学报，41(4):120-125
• 陶永新，张磊，郭丽，陈仁良，连玲丹，谢宝贵，2015.草菇葡萄糖-6-磷酸脱氢酶基因及其可变剪接体的克隆与表达量分析.菌物学报，34(4):724-733
• 王孟兰，赵妍，陈明杰，汪虹，2014.草菇不同菌株木聚糖酶xyn基因的表达及其酶活性分析.菌物学报，33(5):1074-1083
• 谢雨杉，李多伟，李银芳，智彩辉，2015.碘试液比色法测定α-淀粉酶抑制剂活性.江苏农业科学，43(1):301-303
• 徐建中，胡开辉，孙淑静，张俊兰，肖雅敏，林胤煌，2011.不同类型食用菌产木素氧化酶系能力比较与分析.江西农业大学学报，33(2):375-380
• 张介驰，韩增华，张丕奇，孔祥辉，马庆芳，戴肖东，刘佳宁，2013.栽培基质中黑木耳菌丝生长的测定方法研究.食用菌，5:64-65
• 张津京，陈明杰，冯志勇，黄建春，宋晓霞，汪虹，陈辉，2016.芳香族化合物对斑玉蕈菌丝生物量、漆酶活性及其转录水平的影响.菌物学报，35(9):1130-1138
• 张敏，李红，刘娜，张季军，肖千明，孙利平，2013.滑菇菌丝生长速度与子实体产量相关性分析.辽宁农业科学，4:75-76