芳香杯伞生物学特性及驯化栽培Biological characteristics and cultivation of Clitocybe fragrans
顾丹丹;史玲玉;刘红霞;张金秀;姚清国;王立安;
摘要(Abstract)：
以采自于河北省平泉市的一株野生食用菌Clitocybe fragrans的菌丝体为材料，对其营养及环境特性进行研究。检测了不同碳源、氮源、C/N、无机盐在固体培养条件下对芳香杯伞菌丝生长的影响，单因素及正交试验结果表明，实验范围内，芳香杯伞最适碳源为蔗糖、最适氮源为酵母浸粉、最适C/N为40:1、最适无机盐为硫酸镁。正交试验筛选适宜培养基配方为：蔗糖17.3 g，酵母浸粉1.8 g，磷酸二氢钾3 g，无水硫酸镁1.5 g，琼脂20 g，水1 L。对菌丝生长所需的温度、pH及光照条件进行研究，结果显示，适宜菌丝生长的温度范围为19–25℃，pH值范围为5–5.5，光照条件为黑暗。对获得的菌种进行了驯化栽培研究，结果表明，适宜的母种扩繁培养基为：粪草培养料100 g (煮汁)，马铃薯200 g (煮汁)，葡萄糖20 g，磷酸二氢钾3 g，蛋白胨3 g，无水硫酸镁1.5 g，琼脂20 g，水1 L；原种培养基为：高粱粒98%，葡萄糖1%，石膏1%；栽培培养基质为：粪草培养料98%，石膏1%，葡萄糖1%，含水量55%。栽培袋接菌后35–40 d满袋，8–10 d后现原基，7–8 d原基分化成子实体。
关键词(KeyWords)： 芳香杯伞;菌丝体;营养特性;培养基;驯化栽培
基金项目(Foundation): 河北省重点研发计划项目(21326315D,19226910D);; 石家庄学院博士科研启动基金项目(18BS011)~~
作者(Authors): 顾丹丹;史玲玉;刘红霞;张金秀;姚清国;王立安;
DOI: 10.13346/j.mycosystema.210307
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