宋羚;田迪;黄蓉;刘新会;罗家兴;马啸;

• 1:云南农业大学茶学院
• 2:云南农业大学食品科学技术学院
• 3:云南省高原特色农业产业研究院

摘要(Abstract)：

探讨虫草素对非小细胞肺癌细胞株H1781细胞凋亡及迁移的影响及作用机制。培养H1781细胞并分组，对照组用不含药物的培养基处理，虫草素组用含有10、20、30和40μmol/L虫草素处理，处理24h后测定细胞活力，通过显微镜观察细胞形态学，HE染色观察虫草素对细胞整体的影响，细胞免疫荧光技术检测细胞中MMP-9和DAPI核染色情况观察细胞凋亡，Western blotting检测凋亡等相关蛋白表达。与对照组相比，虫草素处理24 h后，H1781细胞系活力显著降低；细胞数量明显减少；HE染色观察发现随着虫草素浓度增加，细胞数量及细胞集团明显变少，免疫荧光技术检测发现药物处理后细胞凋亡明显促进；划痕实验发现虫草素明显降低细胞迁移能力；Western blotting实验中Bax、cleaved caspase-3蛋白表达明显上调，MMP-9、Bcl-2蛋白表达明显下调。虫草素对肺癌H1781细胞迁移有抑制作用、对凋亡有促进作用，推测其作用机制为上调促凋亡蛋白表达、下调抗凋亡蛋白表达。

关键词(KeyWords)： 虫草素;非小细胞肺癌;天然产物;凋亡;迁移;机制研究

Abstract:

Keywords:

基金项目(Foundation): 云南省高层次人才培养支持计划青年拔尖人才专项(YNWR-QNBJ-2018-173);; 云南省重大科技专项：绿色食品国际合作研究中心项目(2019ZG009);; 云南省科技项目(YCJ 2020-323);; 云南科技成果转移支持项目(K2120200001-2101)~~

作者(Authors): 宋羚;田迪;黄蓉;刘新会;罗家兴;马啸;

DOI: 10.13346/j.mycosystema.210469

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