王萌皓;郝正祺;常明昌;孟俊龙;刘靖宇;冯翠萍;

• 1:山西农业大学食品科学与工程学院
• 2:山西省食用菌工程技术研究中心

摘要(Abstract)：

确定广叶绣球菌β-D-葡聚糖对巨噬细胞RAW264.7的免疫调节作用受体,探索广叶绣球菌β-DR葡A聚W2糖64的.7免;T疫LR调4节抗机体制和。TL采R2用抗MT体T分法别测作定用不巨同噬浓细度胞广R叶AW绣2球64菌.7β-1Dh-,葡再聚用糖含对有巨β噬-D细-葡胞聚RA糖W的26X细4.胞7培增养殖á液活培养。收集细胞培养上清和细胞,检测细胞培养上清中NO、IL-6、TNF-α、IFN-β的生成量;提取细胞内总RNA,采用RT-PCR测定巨噬细胞TLR4 mRNA表达量;提取巨噬细胞总蛋白,采用蛋白免疫印迹western blot测定TLR4的蛋白表达。广叶绣球菌β-D-葡聚糖能够促进巨噬细胞RAW264.7增殖,增加NO、IL-6、TNF-α、IFN-β的生成量,提高TLR4mRNA表达和蛋白表达,差异极显著(P<0.01)。TLR4抗体作用细胞后,NO、IL-6、TNF-α、IFN-β的生成量明显下降,差异极显著(P<0.01)。TLR2抗体作用细胞后,NO、IL-6、TNF-α、IFN-β的生成量下降,但差异不显著。广叶绣球菌β-D-葡聚糖可以通过细胞表面受体TLR4激活信号转导通路,增强下游细胞因子的释放,从而调节巨噬细胞RAW264.7的免疫功能。TLR2可能不是广叶绣球菌β-D-葡聚糖的免疫受体。

关键词(KeyWords)： 广叶绣球菌β-D-葡聚糖;巨噬细胞RAW264.7;免疫受体;TLR4抗体;TLR2抗体

Abstract:

Keywords:

基金项目(Foundation): 山西省研究生教育创新项目(2019SY208);; 山西省重点研发计划重点项目(201603D21106);; 山西农谷建设科研专项(SXNGJSKYZX201903);; 食用菌山西省科技创新重点团队;; 黄土高原食用菌提质增效协同创新中心~~

作者(Authors): 王萌皓;郝正祺;常明昌;孟俊龙;刘靖宇;冯翠萍;

DOI: 10.13346/j.mycosystema.200095

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