盛萍萍;刘润进;李敏;

• 1:青岛农业大学菌根生物技术研究所

摘要(Abstract)：

菌根生长状况观察与侵染率测定是菌根学研究中一项重要的基础性工作。综述了丛枝菌根(AM)染色观察与侵染率测定方法研究概况,并对其进行比较和评价。认为采用醋酸墨水染色观察AM生长状况与采用根段侵染率加权法和放大交叉法测定AM真菌侵染率是目前较为科学、准确、易行的方法。根据不同需要也可选择其他适宜的方法,如要了解丛枝发育状况,可采用放大交叉法;如要了解泡囊和侵入点数量,可采用直接计数法,从而使其研究结果具有可比性。有必要建立基于分子生物学技术和脂肪酸定量分析技术测定一种或数种AM真菌侵染状况,这将有力推动AM真菌生理、生态功能研究的发展。

关键词(KeyWords)： 丛枝菌根真菌;染色方法;侵染率;菌根发育;分子技术

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(No.30871737);; “十二五”国家科技支撑项目“北方设施蔬菜高效节能生产关键技术研究与集成”(No.2011BAD12B03);; 青岛市自然科学基金(No.08-1-3-20-jch)

作者(Authors): 盛萍萍;刘润进;李敏;

DOI: 10.13346/j.mycosystema.2011.04.002

参考文献(References)：

• Bainard LD,Klironomos JN,Gordon AM,2011.The mycorrhizal status and colonization of 26tree species growing in urban and rural environments.Mycorrhiza,21(2):91-96
• Bethlenfalvay GJ,Pacovsky RS,Brown MS,1981.Measurement of mycorrhizal infection in soybeans.Soil Science Society of America Journal,45:871-875
• Biermann B,Linderman RG,1981.Quantifying vesicular-arbuscular mycorrhizas:a proposed method towards standardization.New Phytologist,87:63-67
• Brundrett M,Bougher N,Dell B,Grove T,Malajczuk N,1996.Working with mycorrhizas in forestry and agriculture.Australian Centre for International Agricultural Research,32:181-182
• Brundrett MC,PichéY,Peterson RL,1984.A new method for observing the morphology of vesicular-arbuscular mycorrhizae.Canadian Journal of Botany,62:2128-2134
• Cesaro P,van Tuinen D,Copetta A,Chatagnier O,Berta G,Gianinazzi S,Lingua G,2008.Preferential colonization of Solanum tuberosum L.roots by the fungus Glomus intraradices in arable soil of a potato farming area.Applied and Environmental Microbiology,74(18):5776-5783
• Dodd JC,Jeffries P,1986.Early development of vesicular-arbuscular mycorrhizas in autumn-sown cereals.Soil Biology and Biochemistry,18:149-154
• Gai JP,Liu RJ,2000.Ecological distribution of arbuscular mycorrhizal fungi on wild plants in different vegetation regions of Shandong.Chinese Journal of Ecology,19(4):18-22(in Chinese)
• Giovannetti M,Mosse B,1980.An evaluation of techniques for measuring vesicular-arbuscular infection in roots.New Phytologist,84:489-500
• Grace C,Stribley DP,1991.A safer procedure for routine staining of vesicular-arbuscular mycorrhizal fungi.Mycological Research,95:1160-1162
• Graham JH,Drouillard DL,Hodge NC,1996.Carbon economy of sour orange in response to different Glomus spp.Tree Physiology,16:1023-1029
• Hallett PD,Feeney DS,Bengough AG,Rillig MC,Scrimgeour CM,Young IM,2009.Disentangling the impact of AM fungi versus roots on soil structure and water transport.Plant Soil,314:183-196
• Hepper CM,1997.A colorimetric method for estimating vesicular-arbuscular infection in roots.Soil Biology and Biochemistry,9:15-18
• Jacquot E,van Tuinen D,Gianinazzi S,Gianinazzi-Pearson V,2000.Monitoring species of arbuscular mycorrhizal fungi in planta and in soil by nested PCR:application to the study of the impact of sewage sludge.Plant Soil,226:179-188
• Kormanik PP,Bryon WC,Schultz RC,1980.Procedures and equipment for staining large numbers of plant root samples for endomycorrhizal assay.Canadian Journal of Microbiology,26(4):536-538
• Koske RE,Gemma JN,1989.A modified procedure for staining rootsto detect VA mycorrhizas.Mycological Research,92(4):486-505
• Likar M,Regvar M,Mandic-Mulec I,Stres B,Bothe H,2009.Diversity and seasonal variations of mycorrhiza and rhizosphere bacteria in three common plant species at the Slovenian Ljubljana Marsh.Biology and Fertility of Soils,45:573-583
• Liu RJ,Luo XS,1994.A new method to quantify the inoculum potential of arbuscular mycorrhizal fungi.New Phytologist,128:89-92
• McGonigle TP,Miller MH,Evans DG,Fairchild GL,Swan JA,1990.A new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrihizal fungi.New Phytologist,115:495-501
• Newman EI,1966.A method of estimating the total length of root in a sample.Journal of Applied Ecology,3:139-145
• Olsson PA,1999.Signature fatty acids provide tools for determination of distribution and interactions of mycorrhizal fungi in soil.FEMS Microbiology Ecology,29:303-310
• Olsson PA,Bth E,Jakobsen I,1997.Phosphorus effects on the mycelium and storage structures of an arbuscular mycorrhizal fungus as studied in the soil and roots by analysis of fatty acid signatures.Applied and Environmental Microbiology,63:3531-3538
• Phillips JM,Hayman DS,1970.Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection.Transactions of the British Mycological Society,55:158-160
• Su YB,Lin C,Wang SG,2003.Effects of arbuscular mycorrhizal fungi(AMF)on phosphatase in utilizing soil P in the rhizosphere of corn.Journal of Southwest Agricultural University,25(2):115-130(in Chinese)
• Tennant D,1975.A test of a modified line intersection method of measuring root length.Journal of Ecology,63:995-1001
• Trouvelot A,Kough JL,Gianinazzi-Pearson V,1986.Mesure du taux demycorrhization VA d’un système radiculaire.Recherche de méthods d’estimation ayant une signification fonctionelle.In:Gianinazzi-Pearson V,Gianinazzi S(eds.)Physiological and genetical aspects of mycorrhizae.INRA Press,Paris.217-221
• van Aarleand IM,Olsson PA,2003.Fungal lipid accumulation and development of mycelial structures by two arbuscular mycorrhizal fungi.Applied and Environmental Microbiology,69(11):6762-6767
• van Tuinen D,Jacquot E,Zhao B,Gollotte A,Gianinazzi-pearson V,1998.Characterization of root colonization profiles by a microcosm community of arbuscular mycorrhizal fungi using25S rDNA-targeted nested PCR.Molecular Ecology,7(7):879-887
• Vierheilig H,Coughlan AP,Wyss U,Piche Y,1998.Ink and vinegar,a simple staining technique for arbuscular mycorrhizal fungi.Applied and Environmental Microbiology,64(12):5004-5007
• Wang FY,Liu RJ,2002.Effects of biological factors on the diversity of arbuscular mycorrhizal fungi.Acta Ecologica Sinica,22(3):403-408(in Chinese)
• Wang FY,Liu RJ,Lin XG,Zhou JM,2003.Comparison of diversity of arbuscular mycorrhizal fungi in different ecological environments.Acta Ecologica Sinica,23(12):2666-2671(in Chinese)
• Widden P,2001.The use of glycerin jelly for mounting stained roots for the observation and quantification of endomycorrhizal fungi.Mycologia,93(5):1026-1027
• Yang XM,Chen BD,Zhu YG,Wang DM,Wang YS,2008.Effect of arbuscular mycorrhizal fungi Glomus intraradices on growth and mineral nutrition of maize plants in copper contaminated soils.Acta Ecologica Sinica,28(3):1052-1058(in Chinese)
• Yang YN,Ba L,Bai XN,Zhang LC,Wang DL,2010.An improved method to stain arbuscular mycorrhizal fungi in plant roots.Acta Ecologica Sinica,30(3):774-779(in Chinese)
• 盖京苹,刘润进,2000.山东省不同植被区内野生植物根围AM菌的生态分布.生态学杂志,19(4):18-22
• 苏友波,林春,王三根,2003.AM菌根磷酸酶对玉米菌根际土壤磷的影响及其细胞化学定位.西南农业大学学报,25(2):115-130
• 王发园,刘润进,2002.生物因子对AM真菌多样性的影响.生态学报,22(3):403-408
• 王发园,刘润进,林先贵,周健民,2003.几种生态环境中AM真菌多样性比较研究.生态学报,23(12):2666-2671
• 杨秀梅,陈保冬,朱永官,王冬梅,王幼珊,2008.丛枝菌根真菌Glomus intraradices对铜污染土壤上玉米生长的影响.生态学报,28(3):1052-1058
• 杨亚宁,巴雷,白晓楠,张立朝,王德利,2010.一种改进的丛枝菌根染色方法.生态学报,30(3):774-779