苏日娜;Myagmarsuren Erdenedalai;孟根达来;Batkhuu Luvsantseren;Chimedragchaa Chi;哈斯苏荣;

• 1:内蒙古农业大学兽医学院/农业农村部动物疾病诊疗技术重点实验室
• 2:蒙古国传统医学与技术研究所
• 3:内蒙古医科大学附属医院

摘要(Abstract)：

肠道微生物群对宿主健康的影响取决于饮食、环境和宿主等因素。本研究通过Illumina MiSeq平台对冬、夏两季林麝和原麝粪便真菌ITS1区段进行测序,探究原麝和林麝肠道真菌菌群结构特性及季节因素对其多样性的影响。结果表明,林麝和原麝肠道真菌组成中子囊菌门Ascomycota均为优势菌门,它们的相对丰度随物种和季节因素改变差异不显著。但原麝和林麝肠道真菌在α-多样性指数和β-多样性差异显著,表明林麝和原麝肠道真菌结构存在较大差异。此外,研究还表明原麝冬季肠道真菌多样性高于夏季,且菌群丰富度存在显著差异。受季节因素影响,原麝肠道真菌中被孢霉门Mortierellomycota存在显著差异。该研究是国内外首次对原麝肠道真菌组成进行探究,这些结果为改善麝种群的饲养水平提供了理论依据,并为更科学地人工饲养奠定了基础。

关键词(KeyWords)： 原麝;林麝;真菌多样性;季节变化;ITS测序

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划——政府间国际科技创新合作重点专项(2016YFE0116500)~~

作者(Authors): 苏日娜;Myagmarsuren Erdenedalai;孟根达来;Batkhuu Luvsantseren;Chimedragchaa Chi;哈斯苏荣;

DOI: 10.13346/j.mycosystema.210117

参考文献(References)：

• Belanche A,Doreau M,Edwards JE,Moorby JM,Pinloche E,Newbold CJ,2012.Shifts in the rumen microbiota due to the type of carbohydrate and level of protein ingested by dairy cattle are associated with changes in rumen fermentation.The Journal of Nutrition,142(9):1684-1692
• Che Y,Liu K,Li QR,2015.The development of fungal community in intestinal diseases.Parenteral&Enteral Nutrition,22(6):378-381 (in Chinese)
• Chevalier C,Stojanovi?O,Colin DJ,Suarez-Zamorano N,Tarallo V,Veyrat-Durebex C,Rigo D,Fabbiano S,Stevanovi?A,Hagemann S,Montet X,Seimbille Y,Zamboni N,Hapfelmeier S,Trajkovski M,2015.Gut microbiota orchestrates energy homeostasis during cold.Cell,16(6):1360-1374
• Chimedragchaa CH,Mengen Dalai,Batkhuu L,Surong Hasi,Dejidmaa B,Erenechimeg CH,2019.Musk deer(Moschus moschiferus Linnaeus,1758) and study of musk.Mongolia,Ulaanbaater.
• CITES,2002.Consideration of proposals for amendment of appendices I and II.Proposal 11.29.Eleventh Meeting of the Conference of the Parties,Gigiri.
• Fu ZL,Chen L,Li N,2020.Changes of rumen fungi diversity and microflora structure of adult Tan sheep under different feeding modes.Chinese Journal of Animal Science,56(9):209-213 (in Chinese)
• Gock M,Sch?fer M,Perren A,Demartines N,Clavien PA,2005.Fatal esophageal perforation caused by invasive candidiasis.The Annals of Thoracic Surgery,80(3):1120-1122
• Grenet E,Breton A,Barry P,Fonty G,1989.Rumen anaerobic fungi and plant substrate colonization as affected by diet composition.Animal Feed Science Teaches,26(1-2):55-70
• Guo LLZ,Wang LS,2018.The research on gut microbiota dysbiosis in liver cirrhosis patients:current status.Chinese Journal of Microecology,30(1):104-108 (in Chinese)
• Hallen-Adams HE,Kachman DS,Jaehyoung K,Legge MR,Martínez I,2015.Fungi inhabiting the healthy human gastrointestinal tract:a diverse and dynamic community.Fungal Ecology,215:9-17
• Hoffmann C,Dollive S,Grunberg S,Chen J,Li H,Wu GD,Bushman FD,2013.Archaea and fungi of the human gut micro-biome:correlations with diet and bacterial residents.PLo S One,8:e66019
• Holman DB,Chénier MR,2014.Temporal changes and the effect of subtherapeutic concentrations of antibiotics in the gut microbiota of swine.FEMS Microbiology Ecology,90(3):599-608
• Homes V,2004.No licence to kill:the population and harvest of musk deer and trade in musk in the Russian Federation and Mongolia.Nature Reviews.Molecular Cell Biology,2:81
• Hua Y,Lang PF,Wang HT,Wang J,Cao HQ,Jiang GS,2020.Analysis of intestinal fungus diversity of wild and captive North-Chinese leopard (Panthera pardus japonensis) based on high-throughput sequencing.Chinese Journal of Wildlife,241(1):5-14 (in Chinese)
• Kittelmann S,Naylor GE,Koolaard JP,Janssen PH,2012.Aproposed taxonomy of anaerobic fungi (class Neocallimastigomycetes) suitable for large-scale sequence-based community structure analysis.PLo S One,7(5):e36866
• Kohl KD,Skopec MM,Dearing MD,2014.Captivity results in disparate loss of gut microbial diversity in closely related hosts.Conservation Physiology,2(1):cou009
• Kong QH,Liu Y,Suolang SZ,Liu SZ,Tan ZH,Shang P,Shang ZD,2020.Fungal diversity in Tibetan piglet fecal samples.Mycosystema,39(7):1241-1249 (in Chinese)
• Koon HW,Su B,Xu C,Mussatto CC,Tran DH,Lee EC,Ortiz C,Wang J,Lee JE,Ho S,Chen X,Kelly CP,Pothoulakis C,2016.Probiotic Saccharomyces boulardii CNCM I-745 prevents outbreak-associated Clostridium difficile-associated cecal inflammation in hamster.American Journal of Physiology,Gastrointestinal and Liver Physiology,311(4):610-623
• Li H,Qu J,Li T,Yao M,Li J,Li X,2017.Gut microbiota may predict host divergence time during glires evolution.FEMS Microbiology Ecology,3:fix009
• Li YZ,Jiang HQ,Qin YH,2017.Gut fungal microorganisms and inflammatory bowel disease.World Chinese Journal of Digestology,25(17):1528-1535 (in Chinese)
• Lin ZJ,2011.Sequences and phylogeny analysis of ITS and18S r DNA of three species in chlorophyta.Master Thesis,Suzhou University,Suzhou.1-53 (in Chinese)
• Liu G,Lv XY,Zhang MM,Su HJ,Wang YY,2020.The intestinal fungal diversity of wintering black-necked cranes (Grus nigricollis) at Caohai,Guizhou.Heilongjiang Animal Science and Veterinary Medicine,2020(5):128-132+152-153 (in Chinese)
• Qin J,Li R,Raes J,et al.,2010.A human gut microbial gene catalogue established by metagenomic sequencing.Nature,464(7285):59-65
• Qiu X,Zhang F,Yang X,Wu N,Jiang W,Li X,Li X,Liu Y,2015.Changes in the composition of intestinal fungi and their role in mice with dextran sulfate sodium-induced colitis.Scientific Reports,27(5):10416
• Qu W,Zhang Z,Ma JZ,LIU H,YA N.2017.Effect of probiotics on gut microbiota in mice evaluated by high-throughput sequencing.Food Science,38(1):214-219 (in Chinese)
• Richard ML,Lamas B,Liguori G,Hoffmann TW,Sokol H,2015.Gut fungal microbiota:the Yin and Yang of inflammatory bowel disease.Inflammatory Bowel Diseases,21(3):656-665
• Richardson MJ,2001.Diversity and occurrence of coprophilous fungi.Mycological Research,105(4):387-402
• Roeselers G,Mittge EK,Stephens WZ,Parichy DM,Cavanaugh CM,Guillemin K,Rawls JF,2011.Evidence for a core gut microbiota in the zebrafish.The ISMEJournal,5(10):1595-1608
• Schoch CL,Seifert KA,Huhndorf S,Robert V,Spouge JL,Levesque CA,Chen W,Fungal Barcoding Consortium,2012.Nuclear ribosomal internal transcribed spacer(ITS) region as a universal DNA barcode marker for fungi.Proceedings of National Academy of Sciences of the United States of America,109:6241-6246
• Segata N,Izard J,Waldron L,Gevers D,Miropolsky L,Garrett WS,Huttenhower C,2011.Metagenomic biomarker discovery and explanation.Genome Biology,12(6):R60
• Sokol H,Leducq V,Aschard H,Pham HP,Jegou S,Landman C,Cohen D,Liguori G,Bourrier A,Nion-Larmurier I,Cosnes J,Seksik P,Langella P,Skurnik D,Richard ML,Beaugerie L,2017.Fungal microbiota dysbiosis in IBD.Gut,66(6):1039-1048
• Suhr MJ,Banjara N,Hallen-Adams HE,2016.Sequencebased methods for detecting and evaluating the human gut mycobiome.Letters in Applied Microbiology,62(3):209-215
• Tang Y,He L,Xie GZ,Tan ZJ,2019.Progress in research on intestinal fungi.Chinese Journal of Microecology,31(11):1341-1346 (in Chinese)
• Togtokhbayar D,Buyantsog B,Monkhtsog B,2000.Information on musk deer distribution.Proceedings of the Institute of General and Experimental Biology of the Mongolian Academy of Sciences,22:245-246
• Tsendjav D,2002.Mongolian musk deer (Moschus moschiferus Linnaeus,1758).Jinst Cargana Co.Ltd,Ulaanbaatar,Mongolia.
• Yang X,Dai X,Liu L,Wang XW,2014.Fungal diversity in herbivore feces in the Tibetan Plateau.Mycosystema,33(3):621-631 (in Chinese)
• Zheng CL,Cai YH,Wang JM,Wang CX,Zhou L,Feng DY,Yang Y,2020.Investigation report on forest musk deer breeding status in Sichuan Province.Heilongjiang Animal Science and Veterinary Medicine,2020(3):136-139 (in Chinese)
• 车媛，刘凯，李秋荣，2015.真菌菌群在肠道疾病中的作用进展.肠外与肠内营养，22(6):378-381
• 付子琳，陈林，李娜，2020.不同饲养模式下成年滩羊瘤胃真菌多样性及菌群结构的变化.中国畜牧杂志，56(9):209-213
• 郭栗良子，王立生，2018.肝硬化患者肠道菌群失衡的研究现状.中国微生态学杂志，30(1):104-108
• 华彦，郎鹏飞，王洪堂，王姣，曹和琴，姜广顺，2020.基于高通量测序技术对野生和圈养华北豹肠道真菌多样性的分析.野生动物学报，241(1):5-14
• 孔庆辉，刘瑶，索朗斯珠，刘锁珠，谭占坤，商鹏，商振达，2020.藏仔猪粪便真菌菌群多样性分析.菌物学报，39(7):1241-1249
• 李亚周，江慧琴，秦阳华，2017.肠道真菌菌群和炎症性肠病.世界华人消化杂志，25(17):1528-1535
• 林子杰，2011.三种绿藻的ITS和18Sr DNA序列及系统发育分析.苏州大学硕士论文，苏州.1-53
• 刘刚，吕小艳，张明明，粟海军，王野影，2020.贵州草海越冬黑颈鹤的肠道真菌多样性初探.黑龙江畜牧兽医，2011(5):128-132+152-153
• 曲巍，张智，马建章，刘慧，雅男，2017.高通量测序研究益生菌对小鼠肠道菌群的影响.食品科学，38(1):214-219
• 唐圆，贺璐，谢果珍，谭周进，2019.肠道真菌研究进展.中国微生态学杂志，31(11):1341-1346
• 杨顼，戴欣，刘力，王雪薇，2014.青藏高原食草动物粪栖真菌的多样性.菌物学报，33(3):621-631
• 郑程莉，蔡永华，王建明，王承旭，周磊，冯达勇，杨营，2020.四川省林麝养殖现状调查报告.黑龙江畜牧兽医，2020(3):136-139