章轶哲;李宏锡;张凯平;孙承业;张宏顺;卢俊伽;何仟;李海蛟;

• 1:中国疾病预防控制中心职业卫生与中毒控制所
• 2:广东省茂名市野生动物救护研究中心

摘要(Abstract)：

本研究建立了一种基于Taqman-MGB探针的亚稀褶红菇Russulasubnigricans实时荧光定量PCR检测方法。根据亚稀褶红菇与其近似种的内转录间隔区(internal transcribed spacers,ITS)序列差异,设计合成1对引物和1条特异性Taqman-MGB探针,并用常见有毒红菇种类进行验证。结果显示,引物特异性良好,仅亚稀褶红菇出现荧光信号,完成整个检测过程只需2h。该法能够为毒蘑菇中毒的快速检测提供技术支持。

关键词(KeyWords)： 蘑菇中毒;亚稀褶红菇;快速检测;实时荧光定量PCR;Taqman-MGB探针

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31501814)~~

作者(Authors): 章轶哲;李宏锡;张凯平;孙承业;张宏顺;卢俊伽;何仟;李海蛟;

DOI: 10.13346/j.mycosystema.190342

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