刘紫怡;胡文涛;陈辉;唐明;

• 1:华南农业大学林学与风景园林学院岭南现代农业科学与技术广东省实验室

摘要(Abstract)：

彩色豆马勃Pisolithustinctorius可广泛与林木建立外生菌根，其共生可促进植物生长，提高植物对重金属的耐受性。然而，关于彩色豆马勃对重金属锌(Zn)胁迫的生理响应还不完全清楚。本研究在纯培养条件下，测定了Zn胁迫对彩色豆马勃生物量、Zn的活性形态和亚细胞分布，超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性，谷胱甘肽(GSH)、抗坏血酸(AsA)和黑色素含量的影响。结果表明，Zn胁迫抑制了彩色豆马勃的生长，在浓度为563μmol/L时，达到半数最大抑制浓度(IC50)，在浓度为600μmol/L时，生物量降低42.6%。Zn在彩色豆马勃中以活性较低的草酸盐、难溶于水的磷酸盐、果胶酸盐以及与蛋白质呈结合态或吸着态的形式为主。Zn胁迫显著提高了彩色豆马勃菌丝中CAT、POD和SOD活性，增加了GSH和黑色素含量，但对AsA的含量没有显著影响。本研究表明，彩色豆马勃主要通过调节Zn的分布及抗氧化系统，增加黑色素含量，增强对Zn的耐受性，为外生菌根真菌修复重金属污染土壤提供了理论支持。

关键词(KeyWords)： 外生菌根真菌;重金属;黑色素;吸收与固存;抗氧化应激

Abstract:

Keywords:

基金项目(Foundation): 广州市科技计划重点项目(201904020022);; 岭南现代农业科学与技术广东省实验室科研项目(NZ2021025);; 国家自然科学基金(32071639)~~

作者(Authors): 刘紫怡;胡文涛;陈辉;唐明;

DOI: 10.13346/j.mycosystema.210404

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