梁晓洁;刘志华;张平;高暝;吴立文;汪阳东;余孟杨;陈益存;

• 1:中国林业科学研究院亚热带林业研究所
• 2:南京林业大学
• 3:东北林业大学林学院
• 4:清流国有林场

摘要(Abstract)：

油桐是我国重要的木本油料植物,其生产的桐油作为天然的优良干性油在工业上具有广泛用途。但由油桐专化型尖孢镰孢菌Fusarium oxysporum f. sp. fordiis(Fof-1)侵染引起的枯萎病给油桐产业造成了毁灭性灾害,目前尚无有效防治手段。众多实践证明,利用生防菌可以有效防治土传枯萎病。本研究发现,在抗病油桐根围土壤中木霉菌的相对丰度较高,并从中分离获得了16株木霉菌;通过形态学鉴定和ITS-TEF1双基因联合构建系统进化树,鉴定出4种木霉菌:拟康宁木霉Trichodermakoningiopsis(TkonT1)、螺旋木霉T. spirale(TspiT2)、深绿木霉T. atroviride(TatrT3)和哈茨木霉T. harzianum(TharT4);通过对峙培养试验,发现木霉菌株TkonT1、TharT4和TspiT2具有较好的抑菌效果;进一步显微观察发现菌株TkonT1和TatrT3可缠绕在尖孢镰孢菌菌丝体上或穿入菌丝体内营寄生生长,吸收病菌菌丝体养分进而导致病菌菌丝体破裂和细胞原生质消解。结果表明,从抗病油桐根围土壤中获得的拮抗木霉菌株可用于油桐枯萎病的生物防治。

关键词(KeyWords)： 油桐;枯萎病;木霉;抑菌作用

Abstract:

Keywords:

基金项目(Foundation): 中央级公益性科研院所基本科研业务费专项资金(CAFYBB2019ZB002)~~

作者(Authors): 梁晓洁;刘志华;张平;高暝;吴立文;汪阳东;余孟杨;陈益存;

DOI: 10.13346/j.mycosystema.190392

参考文献(References)：

• Bell DK,Wells HD,Markham CR,1982.In vitro antagonism of Trichoderma species against six fungal plant pathogens.Phytopathology,72:379-382
• Benítez T,Rincón,AM,Limón MC,Codón AC,2004.Biocontrol mechanisms of Trichoderma strains.International Microbiology,7(4):249-260
• Bubici G,Kaushal M,Prigigallo MI,Gómez-Lama Cabanás C,Mercado-Blanco J,2019.Biological control agents against Fusarium wilt of banana.Frontiers in Microbiology,10:616
• Bunbury-Blanchette AL,Walker AK,2019.Trichoderma species show biocontrol potential in dual culture and greenhouse bioassays against Fusarium basal rot of onion.Biological Control,130:127-135
• Carbone I,Kohn LM,1999.A method for designing primersets for speciation studies in filamentous ascomycetes.Mycologia,91:553-556
• Chen Y,Yin H,Gao M,Zhu H,Zhang Q,Wang Y,2016.Comparative transcriptomics atlases reveal different gene expression pattern related to Fusarium wilt disease resistance and susceptibility in two Vernicia species.Frontiers in Plant Science,7:1974
• Contreras-Cornejo HA,Macías-Rodríguez L,del-Val E,Larsen J,2019.Interactions of Trichoderma with plants,insects,and plant pathogen microorganisms:chemical and molecular bases.In:Mérillon JM,Ramawat KG(eds.)Co-evolution of secondary metabolites.Springer Nature,Switzerland AG.1-28
• da Silva JAT,de Medeiros EV,da Silva JM,Tenorio DD,Moreira KA,Nascimento TCED,Souza-Motta C,2016.Trichoderma aureoviride URM 5158 and Trichoderma hamatum URM 6656 are biocontrol agents that act against cassava root rot through different mechanisms.Journal of Phytopathology,164(11-12):1003-1011
• Druzhinina IS,Seidl-Seiboth V,Herrera-Estrella A,Horwitz BA,Kenerley CM,Monte E,Mukherjee PK,Zeilinger S,Grigoriev IV,Kubicek CP,2011.Trichoderma:the genomics of opportunistic success.Nature Reviews Microbiology,9(10):749-759
• Edwards J,Johnson C,Santos-Medellín C,Lurie E,Podishetty NK,Bhatnagar S,Eisen JA,Sundaresan V,2015.Structure,variation,and assembly of the root-associated microbiomes of rice.Proceedings of the National Academy of Sciences of the United States of America,112(8):E911-E920
• Gams W,Bissett J,1998.Morphology and identification of Trichoderma.In:Kubicek CP,Harman GE(eds.)Trichoderma and Gliocladium.Vol.1.Taylor&Francis,London.3-34
• Ganuza M,Pastor N,Boccolini M,Erazo J,Palacios S,Oddino C,Reynoso MM,Rovera M,Torres AM,2019.Evaluating the impact of the biocontrol agent Trichoderma harzianum ITEM 3636 on indigenous microbial communities from field soils.Journal of Applied Microbiology,126(2):608-623
• Gava CAT,Pinto JM,2016.Biocontrol of melon wilt caused by Fusarium oxysporum Schlect f.sp.melonis using seed treatment with Trichoderma spp.and liquid compost.Biological Control,97:13-20
• Giurgiu RM,Dumitra?A,Morar G,Scheewe P,Schr?der FG,2018.A study on the biological control of Fusarium oxysporum using Trichoderma spp.,on soil and rockwool substrates in controlled environment.Notulae Botanicae Horti Agrobotanici Cluj-Napoca,46(1):260-269
• Howell CR,2003.Mechanisms employed by Trichoderma species in the biological control of plant diseases:the history and evolution of current concepts.Plant Disease,87:4-10
• Iqbal S,Ashfaq M,Malik AH,Inam-ul-haq,Khan KS,Mathews P,2017.Isolation,preservation and revival of Trichoderma viride in culture media.Journal of Entomology and Zoology Studies,5(3):1640-1646
• Jogaiah S,Abdelrahman M,Tran LP,Ito SI,2018.Different mechanisms of Trichoderma virens-mediated resistance in tomato against Fusarium wilt involve the jasmonic and salicylic acid pathways.Molecular Plant Pathology,19(4):870-882
• Lundberg DS,Lebeis SL,Paredes SH,Yourstone S,Gehring J,Malfatti S,Tremblay J,Engelbrektson A,Kunin V,del Rio TG,Edgar RC,Eickhorst T,Ley RE,Hugenholtz P,Tringe SG,Dangl JL,2012.Defining the core Arabidopsis thaliana root microbiome.Nature,488:86-90
• Macías-Rodríguez L,Guzmán-Gómez A,García-Juárez P,Contreras-Cornejo HA,2018.Trichoderma atroviride promotes tomato development and alters the root exudation of carbohydrates,which stimulates fungal growth and the biocontrol of the phytopathogen Phytophthora cinnamomi in a tripartite interaction system.FEMS Microbiology Ecology,Doi:10.1093/femsec/fiy137
• Mendoza JL,Pérez MI,Prieto JM,Velásquez JD,Olivares JG,Langarica HR,2015.Antibiosis of Trichoderma spp.strains native to northeastern Mexico against the pathogenic fungus Macrophomina phaseolina.Brazilian Journal of Microbiology,46(4):1093-1101
• Mukherjee M,Mukherjee PK,Horwitz BA,Zachow C,Berg G,Zeilinger S,2012.Trichoderma-plant-pathogen interactions:advances in genetics of biological control.Indian Journal of Microbiology,52(4):522-529
• Nawrocka J,Ma?olepsza U,Szymczak K,Szczech M,2018.Involvement of metabolic components,volatile compounds,PR proteins,and mechanical strengthening in multilayer protection of cucumber plants against Rhizoctonia solani activated by Trichoderma atroviride TRS25.Protoplasma,255(1):359-373
• Salas-Marina MA,Isordia-Jasso MI,Islas-Osuna MA,Delgado-Sánchez P,Jiménez-Bremont JF,Rodríguez-Kessler M,Rosales-Saavedra MT,Herrera-Estrella A,Casas-Flores S,2015.The Epl1 and Sm1 proteins from Trichoderma atroviride and Trichoderma virens differentially modulate systemic disease resistance against different life style pathogens in Solanum lycopersicum.Frontiers in Plant Science,6:77
• Samuels GJ,Dodd SL,Gams W,Castlebury LA,Petrini O,2002.Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus.Mycologia,94(1):146-170
• Saravanakumar K,Li Y,Yu C,Wang QQ,Wang M,Sun J,Gao JX,Chen J,2017.Effect of Trichoderma harzianum on maize rhizosphere microbiome and biocontrol of Fusarium stalk rot.Scientific Reports,7(1):1771
• Silva RN,Monteiro VN,Steindorff AS,Gomes EV,Noronha EF,Ulhoa CJ,2019.Trichoderma pathogen plant interaction in pre-harvest food security.Fungal Biology,123(8):565-583
• Wu Q,Sun R,Ni M,Yu J,Li Y,Yu C,Dou K,Ren J,Chen J,2017.Identification of a novel fungus,Trichoderma asperellum GDFS1009,and comprehensive evaluation of its biocontrol efficacy.PLo S One,12(6):e0179957
• Yang SS,Gao M,Zhu HP,Liu YG,Wang YD,Chen YC,2014.Physiological response of Vernicia fordii and V.montana upon inoculation with Fusarium oxysporum.Forest Research,27(6):752-757(in Chinese)
• Yuan ZL,Chen YC,Wang YD,2011.A new leaf blight pathogen of Vernicia fordii.Mycosystema,30(4):658-662(in Chinese)
• Zachow C,Berg C,Müller H,Monk J,Berg G,2016.Endemic plants harbour specific Trichoderma communities with an exceptional potential for biocontrol of phytopathogens.Journal of Biotechnology,235:162-70
• Zhang F,Ge H,Zhang F,Guo N,Wang Y,Chen L,Ji X,Li C,2016.Biocontrol potential of Trichoderma harzianum isolate T-aloe against Sclerotinia sclerotiorum in soybean.Plant Physiology Biochemistry,100:64-74
• Zhang Q,Gao M,Wu L,Wu H,Chen Y,Wang Y,2018.Expression network of transcription factors in resistant and susceptible tung trees responding to Fusarium wilt disease.Industrial Crops and Products,122:716-725
• Zhou C,Wang JJ,Fan HJ,Yang GQ,Liu ZH,2018.Separation and identification of Trichoderma from the rhizosphere soil of Prunus padus.Northern Horticulture,42(12):95-100(in Chinese)
• 杨素素，高暝，朱慧萍，刘英冠，汪阳东，陈益存，2014.三年桐、千年桐感染枯萎病病原菌后的生理反应.林业科学研究，27(6):752-757
• 袁志林，陈益存，汪阳东，2011．一种新发生的油桐叶枯病病原真菌.菌物学报，30(4):658-662
• 周畅，王金杰，范海娟，杨广琦，刘志华，2018.稠李根围木霉的分离及鉴定.北方园艺，42(12):88-95