李姝璇;池玉杰;

• 1:东北林业大学林学院

摘要(Abstract)：

Zn(Ⅱ)-Cys(6)蛋白是一类仅存在于真菌中的锌指转录因子，其在迷宫栓孔菌(曾用名“偏肿革裥菌”) Trametes gibbosa中的相关研究较少。本研究对木屑处理后的迷宫栓孔菌进行转录组测序分析，以期挖掘到差异表达的Zn(Ⅱ)-Cys(6)转录因子，为后续进一步对其功能的分析提供支持。首先，对在木屑处理下的T.gibbosa木质素降解酶进行了酶活检测，确认迷宫栓孔菌能有效降解木质素。之后，用木屑分别处理迷宫栓孔菌0、3、5、7和11d，提取菌丝总RNA，利用高通量测序技术对这5个菌丝样本进行了转录组测序分析。通过COG、GO、Pfam和Swiss等数据库进行功能注释分析，鉴定出迷宫栓孔菌中全部的Zn(Ⅱ)-Cys(6)家族转录因子基因，并进一步筛选出7个差异表达基因。进一步又对这7个差异基因的表达及蛋白结构进行了分析，绘制表达趋势热图，同时也对其理化性质，二、三级结构，疏水性等生物学特性进行了预测。通过构建系统发育树、预测Motif序列等初步分析了它们的进化关系及特性。最后，对这7个基因又利用RT-qPCR技术，进一步验证了转录组测序的结果。本研究从转录水平分析并鉴定出在木屑处理条件下迷宫栓孔菌中7个差异表达的Zn(Ⅱ)-Cys(6)转录因子，研究结果将对后续研究迷宫栓孔菌中Zn(Ⅱ)-Cys(6)转录因子的功能和表达调控机制有重要的参考价值。

关键词(KeyWords)： 迷宫栓孔菌;转录组;Zn(Ⅱ)-Cys(6)转录因子;生物信息学

Abstract:

Keywords:

基金项目(Foundation): 中央高校基本科研业务费专项资金(2572017AA17);; 2018外国文教专家聘请计划高校重点项目(T2018013)~~

作者(Authors): 李姝璇;池玉杰;

DOI: 10.13346/j.mycosystema.210397

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