黄柄钙皮菌无菌培养条件的优化筛选Optimization of axenic culture conditions of Myxomycete Didymium iridis
蒋世翠;张波;李艳双;李玉;
摘要(Abstract)：
黄柄钙皮菌是黏菌中的一种重要模式生物,本文筛选了其无菌培养条件,比较了生活史不同阶段的发生时间,同时比较了生活史中孢子萌发、原质团生长及子实体形成3个阶段的培养条件。结果表明,黄柄钙皮菌适宜的培养条件为:在20%水琼脂上于28℃进行孢子萌发;在24–26℃下20%琼脂+40%燕麦培养基上进行原生质团的培养;在24–26℃下1 000Lx光照10–14h时形成子实体。
关键词(KeyWords)： 黏菌;个体发育;无菌培养
基金项目(Foundation): 国家自然科学基金青年项目(3140010180)~~
作者(Authors): 蒋世翠;张波;李艳双;李玉;
DOI: 10.13346/j.mycosystema.140259
参考文献(References)：
- Aldrich HC,Carroll G,1971.Synaptonemal complexes and meiosis in Didymium iridis:a reinvestigation.Mycologia,63(2):308-316
- Collins ONR,1963.Multiple alleles at the incompatibility locus in the myxomycete Didymium iridis.American Journal of Botany,50(5):477-480
- Collins ONR,1976.Heterothallism and homothallism:a study of 27 isolates of Didymium iridis,a true slime mold.American Journal of Botany,63(2):138-143
- Keller HW,Schoknecht JD,1989.Life cycle of a new annulate-spored species of Didymium.Mycologia,81(2):248-265
- Shi LP,Wang SH,Li Yu,2006.Life cycle of Didymium iridis.Mycosystema,25(3):496-501(in Chinese)
- Yip V,Beekman M,Latty T,2014.Foraging strategies of the acellular slime moulds Didymium iridis and Didymium bahiense.Fungal Ecology,11:29-36
- 史立平,王升厚,李玉,2006.黄柄钙皮菌的生活史.菌物学报,25(3):496-501