谭小珊,王帅杰,吴科元,齐鹏,胡佩雯,张静

• 1:燕山大学环境与化学工程学院
• 2:秦皇岛市第一中学

摘要(Abstract)：

漆酶是一种含铜的单电子多酚氧化酶,能够催化氧化各种酚类及多种染料,在处理染料废水方面具有巨大的潜力。刺芹侧耳Pleurotus eryngii具有较强的产漆酶能力,但漆酶产量在较大程度上受环境条件限制。本文研究了氮源含量、pH、温度、金属离子等环境条件对刺芹侧耳产漆酶能力的影响,优化了其产漆酶条件,并用其粗酶液对典型偶氮类染料甲基橙进行脱色,结果表明,在氮源0.5%(W/W)、pH 5.5、温度28℃、添加5.0mmol/L Mg2+的培养条件下,刺芹侧耳产漆酶能力最强,培养6d时,漆酶酶活可达78.0U/L。用优化培养的刺芹侧耳粗酶液对偶氮染料甲基橙进行脱色,28h后脱色率可达90%,脱色反应为准一级动力学反应,甲基橙并未完全矿化,而是生成小分子中间产物。

关键词(KeyWords)： 刺芹侧耳;漆酶;甲基橙;脱色

Abstract:

Keywords:

基金项目(Foundation): 国家级大学生创新训练计划(201810216028)~~

作者(Author): 谭小珊,王帅杰,吴科元,齐鹏,胡佩雯,张静

DOI: 10.13346/j.mycosystema.190167

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