球孢白僵菌转录因子BbMSN2与其结合基序变体的结合特性分析Interactive assays of the Beauveria bassiana transcription factor BbMSN2 and its binding motif variants
宋驰;郭凌媛;钱斌;Nemat O.Keyhani;冀宏;
摘要(Abstract)：
球孢白僵菌是一种广谱性杀虫真菌，为了探索其转录因子BbMSN2识别启动子核心序列的能力，本研究外源表达并纯化了BbMSN2蛋白，合成了3个含有不同数量核心序列(AGGGG/CCCCT)的核酸探针和6个核心序列点突变的核酸探针，将BbMSN2蛋白和核酸探针体外结合，通过凝胶迁移实验检测核酸探针及结合蛋白的迁移情况。研究发现，目的蛋白与含有核心序列的核酸探针结合时，核酸探针发生了凝胶迁移现象，其中核心序列数量对凝胶迁移的协同效益不显著。但目的蛋白与核心序列点突变核酸探针结合时，凝胶迁移现象明显减弱。上述结果表明，转录因子BbMSN2可以和含有核心序列核酸探针结合并发生相互作用，且对识别序列具有很强的特异性。本研究为深入探索BbMSN2转录调控机制奠定了试验基础。
关键词(KeyWords)： 转录因子;蛋白表达;凝胶迁移实验;蛋白与核酸相互作用
基金项目(Foundation): 苏州市农业科技创新项目(SNG2021006);; 常熟市科技发展计划(农业)项目(CN202110)~~
作者(Authors): 宋驰;郭凌媛;钱斌;Nemat O.Keyhani;冀宏;
DOI: 10.13346/j.mycosystema.220077
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