粘盖乳牛肝菌在低磷、酸铝胁迫下的生长和代谢响应Growth and metabolic responses of Suillus bovinus under low phosphorus and acidic-aluminum stress
冯婧玮;冯万艳;孙学广;
摘要(Abstract):
低磷和酸铝胁迫是酸性土壤限制植物生长的主要因素。有研究指出外生菌根(ectomycorrhiza,ECM)可提高宿主植物对铝毒害和低磷胁迫的适应性。然而,目前有关ECM真菌自身对低磷和酸铝环境的适应机理还不清楚。基于此,本研究以我国南方酸性土壤广泛分布的ECM真菌——粘盖乳牛肝菌Suillusbovinus为研究对象,在纯培养条件下研究了低磷、酸铝胁迫对其生长、营养吸收及菌丝分泌物的影响。结果表明,粘盖乳牛肝菌是一种耐铝型真菌,酸铝胁迫(1 mmol/L)不影响其菌丝生长,而低磷胁迫(20μmol/L)则显著限制其菌丝生长(P<0.05)。值得注意的是,低磷胁迫的抑制效应可被酸铝胁迫逆转。低磷胁迫显著降低了粘盖乳牛肝菌对磷的吸收(P<0.05),而酸铝胁迫则对菌丝钾的吸收有促进作用。低磷、酸铝胁迫同样改变了菌丝分泌物组成。在低磷胁迫下,大量酚酸类、有机酸及脂质代谢物的积累量下调;而酸铝胁迫下则有大量酚酸类物质上调,有机酸和脂质中上调代谢物数量也高于下调数量;低磷酸铝复合胁迫下酚酸和有机酸类代谢物积累量均显著上调。另外,吲哚-3-乙酸(IAA)在各胁迫下均显著上调。以上结果可在一定程度上解释粘盖乳牛菌对低磷、酸铝环境的适应机理,并对后续进一步阐明ECM的共生适应机理有一定指导意义。
关键词(KeyWords): 外生菌根真菌;酸铝;低磷;分泌物
基金项目(Foundation): 贵州省科技计划项目([2018] 5261);; 国家自然科学基金(31971572,31500090);; 贵州大学培育项目([2020] 47)~~
作者(Authors): 冯婧玮;冯万艳;孙学广;
DOI: 10.13346/j.mycosystema.210408
参考文献(References):
- Chai YN,Schachtman DP,2021.Root exudates impact plant performance under abiotic stress.Trends in Plant Science,27(1):80-91
- Chen XL,2009.Research on physiological and ecological mechanism response of Eucalyptus dunnii under low phosphorus stress.Master Thesis,Forestry University,Fuzhou.1-56 (in Chinese)
- Dai GY,Wang LY,Huang LQ,Zheng P,Yao N,2018.Research advance in plant sphingolipid structures,metabolisms and functions.Journal of Plant Physiology,54(12):1748-1762 (in Chinese)
- Felten J,Kohler A,Morin E,Bhalerao RP,Palme K,Martin F,Ditengou FA,LeguéV,2009.The ectomycorrhizal fungus Laccaria bicolor stimulates lateral root formation in poplar and Arabidopsis through auxin transport and signaling.Plant Physiology,151(4):1991-2005
- Feng WY,Zhao YZ,Tan JH,Yang ZQ,Sun XG,2019.Establishment of Pinus massoniana-Suillus bovinus symbiosis.Journal of Microbiology,38(10):1620-1630(in Chinese)
- Gao Y,Wu XQ,Sun MQ,2009.Effects of ectomycorrhizal seedlings of masson pineon absorption and utilization of N,P and K.Journal of Nanjing Forestry University(Natural Science Edition),33(4):77-80 (in Chinese)
- Gerlitz TGM,1996.Effects of aluminium on polyphosphate mobilization of the ectomycorrhizal fungus Suillus bovinus.Plant and Soil,178(1):133-140
- Gu XR,2004.Alleviating of aluminum toxicity by ectomycorrhizal fungi and ectomycorrhizae.Ph DDissertation,Southwest University,Chongqing.1-97 (in Chinese)
- Gu XR,Huang JG,2010.Effect of aluminum on growth,oxalate exudation,and uptake of aluminum,phosphorus and potassium by ectomycorrhizal fungi in vitro.Journal of Ecology,30(2):357-363 (in Chinese)
- Gu XR,Li YN,Jiang YN,Jia H,2018.Effects of Laccaria bicolor on growth,uptake and distribution of nutrients and aluminum of Pinus massoniana seedlings under acid aluminum exposure.Forestry Science,54(2):170-178 (in Chinese)
- Hirano Y,Isomura A,Kaneko S,2003.Root morphology and nutritional status of Japanese red cedar saplings subjected to in situ levels of aluminum in forest soil solution.Journal of Forestry Research,8(3):209-214
- Huby E,Napier JA,Baillieul F,Michaelson LV,Dhondt-Cordelier S,2019.Sphingolipids:towards an integrated view of metabolism during the plant stress response.New Phytologist,225(2):659-670
- Jongbloed RH,Tosserams MWA,Borst-Pauwels GWFH,1992.The effects of aluminium on phosphate uptake by three isolated ectomycorrhizal fungi.Plant and Soil,140(2):167-174
- Kochian LV,Hoekenga OA,Pineros MA,2004.How do crop plants tolerate acid soils?mechanisms of aluminum tolerance and phosphorous efficiency.Annual Review of Plant Biology,55(1):459-493
- Liang C,Pi?eros MA,Tian J,Yao Z,2013.Low p H,aluminum,and phosphorus coordinately regulate malate exudation through Gm ALMT1 to improve soybean adaptation to acid soils.Plant Physiology,161(3):1347-1361
- Manish CS,Mccauley BL,Timperman AT,2007.Aluminum chelation by ectomycorrhizal exudates.Proceeding of the ESA/SER Joint Meeting,48-54
- Morrison EN,Knowles S,Hayward A,Thorn RG,Saville B,Emery RJ,2015.Detection of phytohormones in temperate forest fungi predicts consistent abscisic acid production and a common pathway for cytokinin biosynthesis.Mycologia,107(2):667-692
- Nacry R,Canivenc G,Muller B,2005.A role for auxin redistribution in the responses of the root system architecture to phosphate starvation in Arabidopsis.Plant Physiology,138(4):2061-2074
- Pieterse CMJ,Leon-Reyes A,van der Ent S,van Wees SCM,2009.Networking by small-molecule hormones in plant immunity.Nature Chemical Biology,5(5):308-316
- Policelli N,Bruns TD,Vilgalys R,Nu?ez MA,2019.Suilloid fungi as global drivers of pine invasions.New Phytologist,222(2):714-725
- Raghothama KG,1999.Phosphate acquisition.Annual Review of Plant Physiology and Plant Molecular Biology,2(50):665-693
- Raghothama KG,2000.Phosphate transport and signaling.Current Opinion in Plant Biology,3(3):182-187
- Shen AL,Li XY,Wu SR,1997.The composition characteristics of low-molecular-weight organic acids in soil and their roles on soil material cycling.Plant Nutrition and Fertilizer Science,3(4):363-371
- Silva-Navas J,Conesa CM,Saez A,Navarro-Neila S,Garcia-Mina JM,Zamarre?o AM,Baigorri R,Swarup R,Pozo JC,2019.Role of cis-zeatin in root responses to phosphate starvation.New Phytologist,221(1):242-257
- Song Y,2014.Research on the activation of inorganic Psources by indigenous ECMF in southwest forest district under acid Al stress.Master Thesis,Southwest University,Chongqing.1-58 (in Chinese)
- Sun XG,Feng WY,Li M,Shi J,Ding G,2019.Phenology and cultivation of Suillus bovinus,an edible mycorrhizal fungus,in a Pinus massoniana plantation.Canadian Journal of Forest Research,48(8):960-968
- Tatry MV,Lambilliotte R,CorratgéC,Plassard C,2009.Two differentially regulated phosphate transporters from the symbiotic fungus Hebeloma cylindrosporum and phosphorus acquisition by ectomycorrhizal Pinus pinaster.The Plant Journal,57(6):1092-1102
- Wang J,Zhou ZC,Rao LB,Jin GQ,Li JM,2006.Physiologycal response of liriodendron Chinese provenances with different phosphorus efficiency under low phosphorus stress.Forest Research,19(4):527-531(in Chinese)
- Wang MX,Yuan L,Huang JG,Zhou ZF,2015.Al3+absorption and assimilation by four ectomycorrhizal fungi.Environmental Science,36(9):3479-3485 (in Chinese)
- Wang YH,Chen Z,Zhou JG,Zhang YY,2021.Effects of transformation of Masson pine forest on characteristics of soil acidification and aggregate stability within polluted area of Chongqing.Journal of Ecology,41(13):5184-5194 (in Chinese)
- Watanabe S,Matsumoto M,Hakomori Y,Takagi H,Shimada H,Sakamoto A,2014.The purine metabolite allantoin enhances abiotic stress tolerance through synergistic activation of abscisic acid metabolism.Plant Cell and Environment,37(4):1022-1036
- Winter G,Todd CD,Trovato M,Forlani G,Funck D,2015.Physiological implications of arginine metabolism in plants.Froniters in Plant Science,6:534
- Wu D,Shen H,Yokawa K,Baluska F,2014.Alleviation of aluminium-induced cell rigidity by overexpression of Os PIN2 in rice roots.Journal of Experimental Botany,65(18):5305-5315
- Wu D,Shen H,Yokawa K,Baluska F,2015.Over expression Os PIN2 enhances aluminium internalization by elevating vesicular trafficking in rice root apex.Journal of Experimental Botany,66(21):6791-6801
- Xiong H,2016.Functional analysis of auxin in Arabidopsis primary root elongation in phosphorus starvation response.Master Thesis,Central China Normal University,Wuhan.1-64 (in Chinese)
- Yang HJ,Li Y,Huang JG,2015.Effect of phosphorus supply and signal inhibitors on oxalate efflux in ectomycorrhizal fungi.Journal of Microbiology,55(6):788-795 (in Chinese)
- Yang BZ,Eticha D,Albacete A,Rao IM,Roitsch T,Horst WJ,2012.Physiological and molecular analysis of the interaction between aluminium toxicity and drought stress in common bean (Phaseolus vulgaris).Journal of Experimental Botany,63(8):3109-3125
- Zeng WJ,Peng YL,Zhao XQ,Wu BY,Chen FQ,2019.Comparative proteomics analysis of the seedling root response of drought-sensitive and drought-tolerant maize varieties to drought stress.International Journal of Molecular Sciences,20(11):2793
- Zhang W,Huang JG,Yuan L,Li YB,He LW,2014.Response of ectomycorrhizal fungi to aluminum stress and low potassium soil.Environmental Science,35(10):3863-3868 (in Chinese)
- Zhou Q,2013.Research on response and tolerance mechanism of ectomycorrhizal fungi to aluminum toxicity in low p H condition.Master Thesis,Southwest University,Chongqing.1-66 (in Chinese)
- 陈秀玲,2009.邓恩桉低磷胁迫下生理生态响应机理研究.福建农林大学硕士论文,福州.1-56
- 戴光义,王铃艳,黄骊群,郑萍,姚楠,2018.植物鞘脂结构、代谢和功能的研究进展.植物生理学报,54(12):1748-1762
- 冯万艳,赵燕珍,谭健晖,杨章琦,孙学广,2019.马尾松与粘盖乳牛肝菌菌根共生体形成过程.菌物学报,38(10):1620-1630
- 高悦,吴小芹,孙民琴,2009.马尾松不同菌根苗对氮磷钾的吸收利用.南京林业大学学报(自然科学版),33(4):77-80
- 辜夕容,2004.外生菌根缓解铝毒性的研究.西南大学博士论文,重庆.1-97
- 辜夕容,黄建国,2010.铝对外生菌根真菌草酸分泌及磷、钾、铝吸收的影响.生态学报,30(2):357-363
- 辜夕容,李亚兰,江亚男,贾豪,2018.接种双色蜡蘑对酸性铝胁迫下马尾松幼苗生长、养分和铝吸收与分布的影响.林业科学,54(2):170-178
- 宋影,2014.铝胁迫下西南林区三种土著外生菌根真菌活化利用无机磷源的研究.西南大学硕士论文,重庆.1-58
- 王剑,周志春,饶龙兵,金国庆,李建民,2006.不同磷效率马褂木种源对磷胁迫的生理反应.林业科学研究,19(4):527-531
- 王明霞,袁玲,黄建国,周志峰,2015.4株外生菌根真菌对Al3+吸收与吸附的研究.环境科学,36(9):3479-3485
- 王轶浩,陈展,周建岗,张媛媛,2021.重庆酸雨区马尾松纯林改造对土壤酸化特征及团聚体稳定性的影响.生态学报,41(13):5184-5194
- 熊欢,2016.生长素在拟南芥低磷胁迫应答主根伸长调控中的作用研究.华中师范大学硕士论文,武汉.1-64
- 杨红军,李勇,黄建国,2015.磷与信号抑制剂对外生菌根真菌分泌草酸的调控作用.微生物学报,55(6):788-795
- 张薇,黄建国,袁玲,李阳波,何林卫,2014.外生菌根真菌对Al3+胁迫和低钾土壤的响应.环境科学,35(10):3863-3868
- 周倩,2013.低p H时外生菌根真菌对铝毒的响应及抗(耐)性机理研究.西南大学硕士论文,重庆.1-66