鳞杯伞α-半乳糖苷酶的提取纯化及酶学性质研究Purification and characterization of α-galactosidase from Clitocybe squamulosa
左宁柯;徐丽婧;常明昌;孟俊龙;武斌;王昭玉;
摘要(Abstract)：
采用离子交换层析和凝胶过滤层析对鳞杯伞子实体中的α-半乳糖苷酶进行纯化，得到了一种分子量为50 kDa的α-半乳糖苷酶，命名为CSG。纯化后的CSG纯化倍数为891.46倍，比活力为54.78 U/mg，得率为0.71%。通过BLAST比对液相色谱-串联质谱(LC-MS/MS)获得其肽段，发现其为GH27家族的α-半乳糖苷酶。CSG的最适pH为3.0，最适温度为50℃。在酸性范围pH 2.2–7.0和温度范围4–30℃有较好的稳定性。Mn(2+)、Cd(2+)、Cd(2+)、Cu(2+)、Cu(2+)对CSG有较强的抑制作用。半乳糖和蜜二糖对CSG的抑制类型为混合型抑制。化学修饰剂N-溴代琥珀酰亚胺显著降低CSG的活力，碳二亚胺对CSG具有显著的激活作用。该酶具有良好的蛋白酶抗性，且对棉子糖家族寡糖(RFOs)、瓜尔豆胶和赤槐豆胶均表现出良好的水解作用。
关键词(KeyWords)： α-半乳糖苷酶;鳞杯伞;纯化;酶学性质
基金项目(Foundation): 山西省重点研发计划(201803D221009-1);; 山西省煤基重点科技攻关项目(FT2014-03-01);; 食用菌山西省科技创新重点团队(201805D131009)~~
作者(Authors): 左宁柯;徐丽婧;常明昌;孟俊龙;武斌;王昭玉;
DOI: 10.13346/j.mycosystema.210450
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