赵爽;李婉云;杨静雅;侯怡婷;赵丽莉;贺学礼;

• 1:河北大学生命科学学院

摘要(Abstract)：

利用纤维素酶高产菌绿色木霉Trichoderma viride降解木质纤维素是实现废料资源化的重要手段。本研究选取来自不同生境的两株T.viride,分别以玉米秸秆和甘草药渣为基质,测定两者滤纸纤维素酶(filter paper cellulase,FPase)活性和还原糖产量。从时间、温度、水分、pH 4个方面比较两株T. viride的环境适应性和不同基质的差异性。结果表明,以玉米秸秆为基质,T.virideXJ最适初始料液比为1:4–1:5.5,T. viride AG最适初始料液比为1:5–1:5.5。初始料液比1:5.5时,T. viride AG产FPase活性显著高于T. viride XJ。两株T. viride最适发酵温度均为28℃,各温度处理下不同菌株间无显著差异。两株T. viride均表现为还原糖消耗。以甘草药渣为基质,T. viride XJ最适初始料液比为1:2–1:2.5,T. viride AG最适初始料液比为1:3–1:3.5。料液比高于1:3,T. viride AG产FPase活性显著高于T. viride XJ。T. viride AG最适发酵温度为28℃,T. viride XJ最适发酵温度为23–28℃。温度低于28℃,T. viride XJ产FPase活性显著高于T. viride AG。两株T. viride均表现为还原糖积累。两株T. viride最适初始pH均为6–7,最适发酵时间均为3d。最优发酵条件下FPase活性:T. viride AG>T. viride XJ。对T. viride产FPase诱导能力:甘草药渣>玉米秸秆。变差分解表明两株T.viride产FPase活性差异主要源于菌株对生境的生态适应。比较分析菌种来源、基质类型、环境条件对T. viride发酵效果的影响,将有助于该菌大规模应用性研究。

关键词(KeyWords)： 绿色木霉;固态发酵;滤纸纤维素酶活性;还原糖含量;甘草药渣;玉米秸秆

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31770561)~~

作者(Authors): 赵爽;李婉云;杨静雅;侯怡婷;赵丽莉;贺学礼;

DOI: 10.13346/j.mycosystema.190398

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