李义华;刁红亮;周稳稳;马瑞燕;

• 1:山西农业大学植物保护学院

摘要(Abstract)：

提高虫生真菌孢子应对热胁迫的能力是生防菌应用研究的关键,为研究菌丝培养阶段碳源对玫烟色虫草Cordyceps fumosorosea IF-1106耐热性的影响,选择了麦芽糖、可溶性淀粉、蔗糖、葡萄糖、果糖、海藻糖为碳源的培养基对玫烟色虫草IF-1106进行液体培养,评估了不同碳源条件下菌丝的生长、产孢及所产芽生孢子的耐热性。结果表明,在菌株培养阶段,培养基中碳源的种类及浓度对菌丝产量、产孢量及所产芽生孢子的耐热性有显著影响,其中蔗糖为碳源时,所产芽生孢子的耐热性强,45℃热胁迫条件下LT_(50)为1.65 h;蔗糖浓度为40 g/L时,可产生大量耐热芽生孢子,液体培养3 d后产孢量可达3.43×10⁷个孢子/m L。为探索不同培养条件下所产芽生孢子耐热性不同的原因,提取了孢子内的海藻糖并采用离子色谱法对其进行了定量分析,发现耐热性高的芽生孢子胞内海藻糖含量普遍较低,可见海藻糖是与芽生孢子耐热性密切相关的内源物质。综上所述,选择适宜的培养基是调控孢子耐热性的有效途径,本研究为生产高耐热的玫烟色虫草生防制剂提供了有益的指导。

关键词(KeyWords)： 生防真菌;玫烟色虫草;液体发酵;芽生孢子;耐热性;海藻糖

Abstract:

Keywords:

基金项目(Foundation): 高等学校科技创新项目(2021L093);; 山西省重点研发计划项目(201903D211001-1);; 大同黄花产业发展研究院科研合作项目(2020HXDTHH03)~~

作者(Authors): 李义华;刁红亮;周稳稳;马瑞燕;

DOI: 10.13346/j.mycosystema.210197

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