秦文韬;赵娟;高琳;王守现;刘宇;乔广行;

• 1:北京市农林科学院植物保护研究所
• 2:北京市食用菌工程技术研究中心
• 3:吉林农业大学食药用菌教育部工程研究中心

摘要(Abstract)：

卵孢小奥德蘑Oudemansiella raphanipes是一类依赖覆土栽培的食用菌。本文采用Illumina MiSeq高通量测序技术结合生态学及统计学分析方法研究卵孢小奥德蘑覆土真菌群落特征和影响因素。结果表明，与原始覆土内真菌群落相比，种植卵孢小奥德蘑后的覆土内真菌群落多样性显著下降；在组成方面，虽然优势菌门仍为子囊菌门Ascomycota，但其中的Cladosporium、Acremonium、Gibberella和Emericellopsis等属的相对丰度有所降低，而隶属于被孢霉门Mortierellomycota的Stilbella、Neocosmospora、Pseudogymnoascus和Preussia等属的相对丰度呈增加趋势。卵孢小奥德蘑覆土真菌以腐生营养型为主，与原始覆土相比，种植卵孢小奥德蘑后的粪生营养型真菌和木腐菌呈增加的趋势；进一步研究发现pH、速效钾、速效磷和总氮等覆土土壤理化性质显著影响真菌群落的多样性和结构，pH和速效钾含量与真菌群落的丰富度和多样性呈显著负相关关系。研究结果为深入解析卵孢小奥德蘑覆土微生态机制奠定基础。

关键词(KeyWords)： 卵孢小奥德蘑;覆土;真菌群落;多样性;影响因素

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年项目(32002106);; 国家食用菌产业技术体系(CARS-20);; 北京市乡村振兴科技项目(BJXCZX2022)~~

作者(Authors): 秦文韬;赵娟;高琳;王守现;刘宇;乔广行;

DOI: 10.13346/j.mycosystema.220052

参考文献(References)：

• Bao SD,2000.Soil agrochemical analysis.China Agriculture Press,Beijing.1-495(in Chinese)
• Berendsen RL,Baars JJ,Kalkhove SI,Lugones LG,Sten HA,Bakker PA,2010.Lecanicillium fungicola:causal agent of dry bubble disease in white-button mushroom.Molecular Plant Pathology,11(5):585-595
• Cai WM,Yao HY,Feng WL,Jin QL,Liu YY,Li N,Zheng Z,2009.Microbial community structure of casing soil during mushroom growth.Pedosphere,2009(4):7
• Cao J,Chen YP,Jiang Y,Wu JH,Wang H,2021.Spatial and temporal variation of soil available potassium and its influencing factors in Shaanxi Province.Journal of Soil and Water Conservation,35(5):296-302,311(in Chinese)
• Du N,Hu HP,Xie YZ,Yong TQ,Mo WP,Liang XW,Zhuo LJ,2020.Research progress on Oudemansiella raphanipes.Edible Fungi of China,39(10):1-5,10(in Chinese)
• Feng WL,Jin QL,Fan LJ,Liu JN,Shen YY,Song TT,Tian F,Cai WM,2014.Effects of different casing soil on microbial structure characteristics and yield of Agaricus bisporus.Mycosystema,33(2):425-432(in Chinese)
• Fu YP,Wang XX,Li D,Liu Y,Song B,Zhang CL,Wang Q,Chen MY,Zhang ZW,Li Y,2016.Identification of resistance to wet bubble disease and genetic diversity in wild and cultivated strains of Agaricus bisporus.International Journal of Molecular Sciences,17(10):1568
• Gao Z,Zhang C,Liu H,Zhu YF,Ren ZZ,Jing HJ,Li S,Zhang JJ,Liu XT,Jia L,2018.The characteristics and antioxidation of Oudemansiella radicata selenium polysaccharides on lipopolysaccharide-induced endo-toxemic mice.International Journal of Biological Macromolecules,116:753-764
• Hao YJ,Zhao Q,Wang SX,Yang ZL,2016.What is the radicate Oudemansiella cultivated in China?Phytotaxa,286(1):1-12
• Ke LQ,Li PD,Xu JP,Wang QS,Wang LL,Wen HP,2019.Microbial communities and soil chemical features associated with commercial production of the medicinal mushroom Ganoderma lingzhi in soil.Scientific Reports,9:15839
• Kunin V,Engelbrektson A,Ochman H,Hugenholtz P,2010.Wrinkles in the rare biosphere:pyrosequencing errors can lead to artificial inflation of diversity estimates.Environmental Microbiology,12(1):118-123
• Li ZW,Dai JJ,Li SJ,Wu YY,Fan JP,Cheng PP,Li X,2018.Research on changes of mineral elements in the cultivation process of Morchella rufobrunnea.Molecular Plant Breeding,16(24):8214-8218(in Chinese)
• Mc Gee CF,2018.Microbial ecology of the Agaricus bisporus mushroom cropping process.Applied Microbiology and Biotechnology,102(3):1075-1083
• Monard C,Gantner S,Stenlid J,2013.Utilizing ITS1 and ITS2 to study environmental fungal diversity using pyrosequencing.FEMS Microbiology Ecology,84(1):165-175
• Natvig DO,Taylor JW,Tsang A,Hutchinson MI,Powell AJ,2015.Mycothermus thermophilus gen.et comb.nov.,a new home for the itinerant thermophile Scytalidium thermophilum (Torula thermophila).Mycologia,107(2):319-327
• Pecchia J,Cortese R,Albert I,2014.Investigation into the microbial community changes that occur in the casing layer during cropping of the white button mushroom,Agaricus bisporus.In:Singh M (ed.) Proceedings of the8th International Conference on Mushroom Biology and Mushroom Products,New Delhi.309-313
• Plassart P,Prévost-BouréNC,Uroz S,Dequiedt S,Stone D,Creamer R,Grifths RJ,Bailey MJ,Ranjard L,Lemanceau P,2019.Soil parameters,land use,and geographical distance drive soil bacterial communities along a European transect.Scientific Reports,9:605
• Ren F,Zhang YG,Yu H,Zhang YA,2020.Ganoderma lucidum cultivation affect microbial community structure of soil,wood segments and tree roots.Scientific Reports,10(1):3435
• Seifert KA,Louis-Seize G,Samson RA,Boekhout T,1997.Remersonia,a new genus for Stilbella thermophila,a thermophilic mould from compost.Canadian Journal of Botany,75(7):1158-1165
• Siyoum NA,Surridge K,Linde EJ,Korsten L,2016.Microbial succession in white button mushroom production systems from compost and casing to a marketable packed product.Annals of Microbiology,66(1):151-164
• Straatsma G,Samson RA,Olijinsma TW,den Camp HJO,Gerrits JP,van Griensven LJ,1994.Ecology of thermophilic fungi in mushroom compost,with emphasis on Scytalidium thermophilum and growth stimulation of Agaricus bisporus mycelium.Applied and Environmental Microbiology,60(2):454-458
• Tan H,Yu Y,Tang J,Liu TH,Miao RY,Huang ZQ,Martin FM,Peng WH,2021.Build your own mushroom soil:microbiota succession and nutritional accumulation in semi-synthetic substratum drive the fructification of a soil-saprotrophic morel.Frontiers in Microbiology,12:656656.
• Wu F,Zhou LW,Yang ZL,Bau T,Li TH,Dai YC,2019.Resource diversity of Chinese macrofungi:edible,medicinal and poisonous species.Fungal Diversity,98:1-76
• Wu QY,Zhang YR,Wei J,Kang LH,2019.Effects of different covering materials on the yield and quality of Oudemansiella radicata.Edible Fungi of China,38(10):19-21(in Chinese)
• Yuan Y,Huang HC,Ye LY,Fu JS,Wu XP,2019.Analysis of fungal community in continuous cropping soil of Ganoderma lucidum.Mycosystema,38(12):2112-2121(in Chinese)
• Zhou JM,Bai XM,Zhao RL,2017.Microbial communities in the native habitats of Agaricus sinodeliciosus from Xinjiang Province revealed by amplicon sequencing.Scientific Reports,7:15719
• 鲍士旦，2000.土壤农化分析.北京：中国农业出版社.1-495
• 曹婧，陈怡平，江瑶，毋俊华，王洪，2021.陕西省农田土壤速效钾时空变化及其影响因素.水土保持学报,35(5):296-302,311
• 杜娜，胡惠萍，谢意珍，雍天乔，莫伟鹏，梁晓薇，卓丽君，2020.卵孢小奥德蘑的研究进展.中国食用菌，39(10):1-5,10
• 冯伟林，金群力，范丽军，刘佳楠，沈颖越，宋婷婷，田芳，蔡为明，2014.不同覆土基质微生物结构特征研究及其对双孢蘑菇产量的影响.菌物学报，33(2):425-432
• 黎智文，代俊杰，李寿建，吴英勇，范君佩，成盼盼，李晓，2018.变红羊肚菌栽培过程中矿物质元素变化分析.分子植物育种，16(24):8214-8218
• 吴秋云，张玉蓉，魏娇，康雷航，2019.不同覆土材料对黑皮鸡枞产量和品质的影响.中国食用菌，38(10):19-21
• 袁源，黄海辰，叶丽云，傅俊生，吴小平，2019.灵芝连作土壤真菌群落分析.菌物学报，38(12):2112-2121