丰磊;李晓然;付鸣佳;黄紫妍;魏伟群;肖世平;

• 1:江西师范大学生命科学学院
• 2:江西天佳生物工程股份有限公司

摘要(Abstract)：

生物钟的节律振荡器主要成分之间的关系构成了转录-翻译负反馈环，并以此调控生物体的生理生化反应和生长发育等。以不同时间的蓝光照射和蛋白酶体抑制剂MG132处理蛹虫草菌丝体，通过实时荧光PCR分析其中节律振荡器主要成分的3个基因Cmfrq、Cmwc-1和Cmwc-2转录水平变化，以期确定3个基因在蛹虫草中的相互关系和变化规律。研究表明，持续的黑暗情况下，MG132对Cmfrq转录水平的影响比较大，对Cmwc-1和Cmwc-2转录水平的影响有限。在黑暗培养过程中2 h的蓝光间断条件下，无MG132处理培养后期Cmfrq转录水平急剧下降，Cmwc-1转录水平却急剧上升，Cmfrq和Cmwc-1转录水平呈现生物振荡器负反馈调控特点；Cmfrq转录水平受营养条件的影响，加MG132处理后使得Cmfrq转录对营养的改变不敏感。持续的蓝光照射培养条件下，无MG132处理时Cmfrq转录水平在后期也出现急剧的下降，Cmfrq转录对MG132处理更为敏感；无论有或无MG132处理，这3个基因之间均没有反映出生物振荡器负反馈环的特点。在蓝光12h和黑暗12h的周期交替条件下，前后2个周期这3个基因的转录水平出现不一致的情况，由持续黑暗进入周期交替时，Cmfrq转录水平由高到低；在第2个周期时Cmwc-1和Cmwc-2转录水平均非常低，但MG132导致Cmwc-1和Cmwc-2转录水平升高。与黑暗条件相比，蓝光处理可以导致Cmfrq转录水平升高。Cmwc-1转录水平在多数情况下均非常低，表明该基因是生物振荡器中的关键基因。

关键词(KeyWords)： 蛹虫草;蓝光;蛋白酶体抑制剂;基因转录水平

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31760601);; 南昌市重大科技攻关项目(2020)~~

作者(Authors): 丰磊;李晓然;付鸣佳;黄紫妍;魏伟群;肖世平;

DOI: 10.13346/j.mycosystema.210352

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