基于CdSe阳离子交换的玉米赤霉烯酮新型荧光免疫检测方法的建立及应用Sensitive fluorescence immunoassay for the detection of zearalenone base on signal amplification induced by cation exchange in CdSe quantum dots
章先;徐浩瑜;赵鹿如;李可;张晓峰;程昌勇;宋厚辉;
摘要(Abstract)：
作为镰刀属真菌的次级代谢产物，玉米赤霉烯酮(zearalenone,ZEN)具有强烈的生殖毒性和免疫毒性，严重威胁动物和人类健康。本研究通过采用羧基修饰的CdSe水溶性量子点(quantum dots,QDs)标记ZEN单克隆抗体，并基于CdSe阳离子交换信号增强原理，建立了ZEN新型荧光免疫检测方法(CdSe QDs-FLISA)，检测下限(IC_(10))和半数抑制率(IC_(50))分别为0.006 ng/mL和0.17 ng/mL，检测区间(IC20–IC80)为0.01–0.45 ng/mL。与ZEN的结构类似物(α-zearalanol、zearalanone、α-zearalenol、β-zearalenol and β-zearalanol)交叉反应性依次为22.3%、13.1%、6.2%、1.6%和3.9%，与农产品中其他真菌毒素如黄曲霉毒素B_1(AFB_1)、赭曲霉毒素A(OTA)、呕吐毒素(DON)和伏马毒素B_1(FB_1)几乎不存在交叉反应。该方法在玉米样本中加标回收率较高，且实际样本中ZEN的定量检测结果与LC-MS/MS一致性较好。本研究建立的CdSe QDs-FLISA操作简单，可实现对样本中ZEN的快速定量检测与分析，便于基层单位推广使用，具有较好的应用前景，同时也可为其他病原微生物检测技术的开发提供参考。
关键词(KeyWords)： 真菌毒素;单克隆抗体;量子点;信号增强;定量检测
基金项目(Foundation): 浙江省重点研发计划(2021C02058);; 国家自然科学基金(32002358);; 国家级大学生创新创业训练计划项目(202010341040)~~
作者(Authors): 章先;徐浩瑜;赵鹿如;李可;张晓峰;程昌勇;宋厚辉;
DOI: 10.13346/j.mycosystema.210421
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