饶佳;鲍大鹏;李燕;汪滢;程向阳;王莹;

• 1:国家食用菌工程技术研究中心农业部南方食用菌资源利用专业重点实验室上海市农业遗传育种重点开放实验室上海市农业科学院食用菌研究所
• 2:上海海洋大学食品科学与工程学院

摘要(Abstract)：

在前期工作中发现草菇含有30个GH61家族基因同源物(Vv_gh61_1至Vv_gh61_30),进一步分析了这些基因的结构特点以及其编码蛋白基本性质和系统进化关系,并研究了Cu⁽²⁺⁾和Mn(2+)和Mn⁽²⁺⁾对基因表达水平的影响。分析表明有17个草菇GH61基因串联成6个基因簇,存在明显的串联重复现象,系统发育树与基因外显子位置分析表明串联重复基因分布在同一进化分枝上并具有相似的基因结构,串联重复基因编码序列一致性在71%–94%之间。草菇GH61编码蛋白的氨基酸数目在217–442aa之间,分子量和等电点分别介于22.4–45.4k Da和5.2–9.3之间,绝大多数都含有信号肽并定位在细胞外,都含有Glyco_hydro_61功能域以及CBM1、peroxidase等多样化的功能域,系统进化树表明草菇GH61家族基因具有3个主要进化分枝,与灰盖鬼伞等的GH61基因有较近的进化关系。金属离子诱导作用显示,Mn2+对草菇GH61家族基因的表达水平存在诱导作用而Cu2+的诱导作用不明显。

关键词(KeyWords)： 草菇;GH61家族;串联重复;基因表达

Abstract:

Keywords:

基金项目(Foundation): 上海市科技人才计划项目(13XD1424700)~~

作者(Authors): 饶佳;鲍大鹏;李燕;汪滢;程向阳;王莹;

DOI: 10.13346/j.mycosystema.150014

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