药用真菌香樟范氏孔菌的驯化栽培及生物学发育特征Cultivation and biological characteristics of medical fungus Vanderbylia cinnamomea
杨雄;赵长林;
摘要(Abstract):
本研究旨在实现香樟范氏孔菌Vanderbylia cinnamomea的袋料栽培驯化及筛选其最佳培养配方,揭示子实体不同生长阶段生物学发育特征。选取工业大麻秸秆、天麻废材、杨木、栎木为培养料,共设置8种配方开展试验,期间观察不同时期子实体生长发育特征。香樟范氏孔菌驯化试验结果表明:工业大麻秆和天麻废材无法实现香樟范氏孔菌的袋料栽培,而工业大麻秆配方可产大量菌丝但不产生子实体,其菌丝在基质上开始生长时间短、生长速度快;以杨木、栎木为栽培基质可产生子实体,实现该药用真菌的栽培驯化;结实配方为栎木屑70%、玉米芯20%、辅料10%,温度25℃左右,菌丝满袋后全开口开袋,湿度为90%–95%,早晚通风一次,补充散射光,该条件下成功驯化最大体积子实体。子实体不同生长阶段的生物学生长发育特征揭示,子实体原基呈白色凸起状,继而呈球状,后体积渐膨大,渐生成菌孔,其颜色由白变浅黄至褐色,32 d后停止生长,子实体发育成熟。
关键词(KeyWords): 木生真菌;药用真菌;子实体;多孔菌;西南地区
基金项目(Foundation): 云南省科技厅基础研究重点项目(202001AS070043)~~
作者(Authors): 杨雄;赵长林;
DOI: 10.13346/j.mycosystema.210203
参考文献(References):
- Chen JZ,Yang X,Zhao CL,2020.Vanderbylia cinnamomea sp.nov.from southwestern China.Mycotaxon,135:371-382
- Cui BK,Li HJ,Ji X,Zhou JL,Song J,Si J,Dai YC,2019.Species diversity,taxonomy and phylogeny of Polyporaceae(Basidiomycota) in China.Fungal Diversity,97:137-302
- Dai YC,Yang ZL,Cui BK,Wu G,Yuan HS,Zhou LW,He SH,Ge ZW,Wu F,Wei YL,Yuan Y,Si J,2021.Diversity and systematics of the important macrofungi in Chinese forests.Mycosystema,40(4):770-805 (in Chinese)
- Dai YC,Zhou LW,Yang ZL,Wen HA,Bau T,Li TH,2010.Arevised checklist of edible fungi in China.Mycosystema,29(1):1-21 (in Chinese)
- Dong CH,Liu QZ,Zhang JJ,2017.Research progress on important edible and medicinal fungi in China over the last decade.Journal of Microbiology,37(4):1-9 (in Chinese)
- Feng XF,Zhao N,Sun ZY,Li J,Chen YH,Yang B,2020.Study on the 3 kinds of edible fungi culture with the wood residues of Gastrodia elata.Journal of Southwest Forestry University(Natural Sciences),40(4):163-168(in Chinese)
- Gao Y,Li Y,2013.Selected biological characteristics and artificial cultivation of Polyporus brumalis.Acta Edulis Fungi,20(2):25-27 (in Chinese)
- Guo L,Wang YW,Zhang SB,Liu S,Wang XL,Liu GQ,2021.A preliminary study on co-fermentation of Sanghuangporus lonicericola and Cordyceps militaris to produce fungal polysaccharides.Mycosystema,40(4):965-976 (in Chinese)
- Li C,Wang XL,Chen T,Wang W,Yang QF,2020.Trametes robiniophila Murr.in the treatment of breast cancer.Biomedicine&Pharmacotherapy,128:110254
- Li WJ,Dong CH,Liu XZ,Li QP,Xia JM,Liang L,2016.Research advances in artificial cultivation of Chinese cordyceps.Mycosystema,35(4):375-387 (in Chinese)
- Liu CJ,Zhang XY,Dai YC,Wei YL,2005.Artifical culture of Phellinus baumii.Mycosystema,24(4):306-309(in Chinese)
- Ma L,Bao HY,Han Y,Hu RW,Bau T,Li Y,2019.Cultivation and biological characteristics of Trametes suaveolens.Mycosystema,38(2):187-194 (in Chinese)
- Song X,Li Y,Zhang H,Yang Q,2015.The anticancer effect of huaier.Oncology Reports,34:12-21
- Tang XL,Ma LF,2010.Physical properties and chemical composition of a hemp core.Journal of Zhejiang Forestry College,27(5):794-798 (in Chinese)
- Tang XM,Feng YL,Guo X,Ma M,Chen ZQ,Wu SR,2019.Current situation and development trend of edible medicinal fungi in China.Northern Horticulture,2019(8):148-153 (in Chinese)
- Wang H,Chen CB,Zhang B,Tong XD,Wang SM,Li Y,2019.Antioxidant activities and extraction technique optimization of crude polysaccharides from the fruiting body of Floccularia luteovirens.Mycosystema,38(10):1681-1688 (in Chinese)
- Wang H,Qian K,Si J,Cui BK,2021.Research advances on polysaccharides from sanghuang.Mycosystema,40(4):895-911 (in Chinese)
- Wang T,Bao HY,Bau T,Li Y,2016.Biological characteristics and cultivation of Inonotus hispidus parasitizing on Ulmus macrocarpa var.mongolica.Mycosystema,35(6):694-704(in Chinese)
- Wang YX,Yuan HS,2021.Research progress on chemical constituents and anti-tumor effects of Huai’er(Vanderbylia robiniophila).Mycosystema,40(3):411-422(in Chinese)
- Wu F,Yuan HS,Zhou LW,Yuan Y,Cui BK,Dai YC,2020.Polypore diversity in South China.Mycosystema,39(4):653-681 (in Chinese)
- Wu F,Zhou LW,Yang ZL,Bau T,Li TH,Dai YC,2019.Resource diversity of Chinese macrofungi:edible,medicinal and poisonous species.Fungal Diversity,98:1-76
- Zhao Y,Liu Y,2018.A mechanistic overview of herbal medicine and botanical compounds to target transcriptional factors in breast cancer.Pharmacological Research,130:292-302
- 戴玉成,杨祝良,崔宝凯,吴刚,袁海生,周丽伟,何双辉,葛再伟,吴芳,魏玉莲,员瑗,司静,2021.中国森林大型真菌重要类群多样性和系统学研究.菌物学报,40(4):770-805
- 戴玉成,周丽伟,杨祝良,文华安,图力古尔,李泰辉,2010.中国食用菌名录.菌物学报,29(1):1-21
- 董彩虹,刘奇正,张娇娇,2017.近十年中国重要食药用菌研究进展.微生物学杂志,37(4):1-9
- 冯小飞,赵宁,孙紫宇,李俊,陈玉惠,杨斌,2020.利用天麻废弃菌材栽培3种食用菌的试验.西南林业大学学报(自然科学版),40(4):163-168
- 高源,李玉,2013.冬生多孔菌生物学特性及驯化.食用菌学报,20(2):25-27
- 郭璐,王雅葳,张少冰,刘帅,王晓玲,刘高强,2021.忍冬桑黄和蛹虫草共发酵联产真菌多糖初步研究.菌物学报,40(4):965-976
- 李文佳,董彩虹,刘杏忠,李全平,夏金明,梁蕾,2016.冬虫夏草培植技术研究进展.菌物学报,35(4):375-387
- 刘春静,张晓阳,戴玉成,魏玉莲,2005.鲍氏层孔菌培养条件的研究.菌物学报,24(4):306-309
- 马列,包海鹰,韩玉,胡日瓦,图力古尔,李玉,2019.香栓菌的驯化栽培及生物学发育特征.菌物学报,38(2):187-194
- 汤昕明,冯云利,郭相,马明,陈正启,吴素蕊,2019.我国食药用菌栽培现状分析及展望.北方园艺,2019(8):148-153
- 唐晓莉,马灵飞,2010.大麻秆芯的物理性质和化学组分.浙江林学院学报,27(5):794-798
- 王豪,钱坤,司静,崔宝凯,2021.桑黄类真菌多糖研究进展.菌物学报,40(4):895-911
- 王欢,陈长宝,张波,佟希丹,王淑敏,李玉,2019.响应面法优化黄绿卷毛菇子实体多糖的提取工艺及其体外抗氧化活性研究.菌物学报,38(10):1681-1688
- 王婷,包海鹰,图力古尔,李玉,2016.寄生于蒙古黄榆上的粗毛纤孔菌生物学特性及驯化栽培.菌物学报,35(6):694-704
- 王雨曦,袁海生,2021.槐生拜尔孔菌(槐耳)的化学成分及其抗肿瘤活性研究进展.菌物学报,40(3):411-422
- 吴芳,袁海生,周丽伟,员瑗,崔宝凯,戴玉成,2020.中国华南地区多孔菌多样性研究.菌物学报,39(4):653-681