响应面法优化黄绿卷毛菇子实体多糖的提取工艺及其体外抗氧化活性研究Antioxidant activities and extraction technique optimization of crude polysaccharides from the fruiting body of Floccularia luteovirens
王欢,陈长宝,张波,佟希丹,王淑敏,李玉
摘要(Abstract)：
以采自四川石渠的黄绿卷毛菇Floccularialuteovirens为研究对象,采用水提醇沉的经典方法以及单因素试验,并利用Box-BenhnkenDesign(BBD)中心组合试验设计原理,以提取温度、时间以及料液比3个影响因素作为自变量,多糖提取率为考察指标,设计3因素3水平的响应面试验,优化黄绿卷毛菇子实体多糖(FLPs)的提取工艺,并通过·OH和O2-·自由基清除能力考察FLPs的抗氧化能力。最终确定最优水提工艺为提取温度89.31℃,提取时间5.08h,料液比1:48.54(g/mL),且FLPs具有良好的·OH和O2-·自由基清除能力,具有较强的体外抗氧化活性。同时,验证试验证明了该设计方法和模型的准确性和可行性,且在该工艺条件下,多糖提取率有所提高,可为其多糖功能性食品和药品的开发提供理论依据。
关键词(KeyWords)： 黄绿卷毛菇;真菌多糖;响应面法;提取工艺;自由基清除
基金项目(Foundation): 高等学校学科创新引智计划“111”合作基地（D17014）;; 农业部公益性行业科研专项(201503137)~~
作者(Author): 王欢,陈长宝,张波,佟希丹,王淑敏,李玉
DOI: 10.13346/j.mycosystema.190137
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