张莹;胡佳续;滕少娜;廖芳;罗加凤;

• 1:天津海关动植物与食品检测中心
• 2:重庆海关技术中心

摘要(Abstract)：

采用常规平板分离法,从一批进境的美国大豆样品中获得1株可疑的间座壳属菌株MDD57。经形态学观察发现,该菌株在PDA培养基上产生分生孢子器,且同时产生大量α型和β型分生孢子,未见有性阶段。经ITS和tef1α基因扩增、核酸序列比对分析,发现该菌株同GenBank中2株Diaporthe novem菌株的基因序列同源性达100%,且聚类于同一发育分支。经柯赫氏法则验证,该菌株可侵染大豆茎部和叶片,引起典型的间座壳属病害症状。根据以上结果,确定进境美国大豆中携带有D.novem,这是天津口岸首次从美国大豆中截获该危险性病菌。该病菌同大豆北方茎溃疡病菌、大豆南方茎溃疡病菌、大豆拟茎点种腐病菌同为近似种,引起大豆茎秆溃疡及种子腐烂,危害严重。

关键词(KeyWords)： Diaporthe novem;形态特征;序列分析;致病性

Abstract:

Keywords:

基金项目(Foundation): 海关总署科研项目(2020HK171)~~

作者(Authors): 张莹;胡佳续;滕少娜;廖芳;罗加凤;

DOI: 10.13346/j.mycosystema.210211

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