丛枝菌根真菌培养方法研究进展ADVANCE OF CULTURAL METHODS FOR ARBUSCULAR MYCORRHIZAL
杨晓红,孙中海,邵菊芳,仝瑞建
摘要(Abstract):
文章结合最新研究成果着重从十个不同的层面对丛枝菌根真菌(AM真菌)的培养方法研究做了较为系统的介绍与评述。认为活体宿主植物盆栽培养法是最简单、最容易、也是最可靠的AM真菌培养方法,玻璃珠分室培养可方便地将培养基质与AM真菌分开,能获得纯净的AM真菌菌体,具有其它方法不可替代的作用,AM真菌单孢无菌的分室Ri T-DNA转型胡萝卜根双重离体培养是获得AM真菌纯净菌体和研究AM真菌遗传、生理、生化等特性的理想方法,以此方法为基础,在根室中补充碳源、在菌丝生长室置换培养基、多次收获菌体的转型根改良双重培养法是提高某些AM真菌孢子产量的力荐方法。
关键词(KeyWords): 最新进展;评述
基金项目(Foundation): 中国博士后科学基金资助(NO:8418)
作者(Author): 杨晓红,孙中海,邵菊芳,仝瑞建
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