红托竹荪多糖诱导肿瘤细胞凋亡的作用初探Induction of Dictyophora rubrovolvata polysaccharides on tumor cell apoptosis
叶建方;罗鹏;肖佳艳;吴雪艳;张豫;
摘要(Abstract)：
探讨红托竹荪多糖对小鼠腹水瘤S180细胞凋亡的作用。分别使用不同浓度的红托竹荪多糖处理S180细胞24h,Western blot法检测Bcl-xl、Bax、caspase-9和Caspase-3等蛋白表达,使用流式细胞仪检测细胞凋亡。Western blot法检测结果显示Bcl-xl表达量随竹荪多糖剂量增加而降低,Bax表达量则相反,每组Bax/Bcl-xl的比值增加,Caspase-9、Caspase-3表达量增加;流式细胞仪检测结果显示:0、25、50和100mg/L组细胞凋亡率分别为5.12%±0.11%、9.61%±0.61%、16.39%±0.19%和17.05%±0.13%,与对照组相比细胞凋亡率增高,差异具有统计学意义(P<0.05),推断红托竹荪多糖具有诱导S180细胞凋亡的功能。
关键词(KeyWords)： 红托竹荪;B细胞淋巴瘤/白血病-XL蛋白;B细胞淋巴瘤/白血病-2相关X蛋白;半胱氨酸天冬氨酸特异性蛋白酶-3;半胱氨酸天冬氨酸特异性蛋白酶-9
基金项目(Foundation): 国家自然科学基金(81160336);; 贵州省科技厅农业攻关计划项目(黔科合NY字[2011]3055号);贵州省科技厅(黔科合J字[2010]2188号);; 贵州省教育厅基金项目(黔省专合字[2010]39号)~~
作者(Authors): 叶建方;罗鹏;肖佳艳;吴雪艳;张豫;
DOI: 10.13346/j.mycosystema.150128
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