陈金辉;甘永健;崔国兵;邓懿祯;

• 1:华南农业大学植物保护学院广东省微生物信号与病害防控重点实验室群体微生物研究中心

摘要(Abstract)：

为了研究核转运蛋白SsKapJ在甘蔗鞭孢堆黑粉菌中发挥的作用,本研究利用PEG介导的原生质体转化方法获得基因敲除突变体,并进行了相关生物学表型分析。结果显示潜在核转运蛋白基因SsKapJΔ突变体有性配合和菌丝生长显著减弱,转录组分析SsKapJ参与环磷酸腺苷/蛋白激酶A(cAMP/PKA)和丝裂原活化蛋白激酶(MAPK)信号途径调控。另一方面,SsKapJΔ突变体担孢子对吡唑醚菌酯杀真菌剂敏感性增强,但突变体对苯菌灵杀真菌剂敏感性以及环境胁迫测试(渗透压、细胞壁抑制剂、氧化还原和乙醇)中较野生型没有明显变化。本研究结果揭示了SsKapJ核转运蛋白在甘蔗鞭孢堆黑粉菌的有性配合和菌丝生长过程中具有重要调控作用,为后续开展核运输途径调控真菌有性配合/二态型转变研究提供基础。

关键词(KeyWords)： 甘蔗鞭孢堆黑粉菌;有性配合;核转运蛋白;杀真菌剂;菌丝生长

Abstract:

Keywords:

基金项目(Foundation): 广州市科技计划重点项目:甘蔗鞭黑穗病生物防治技术的研发与应用(201904020010)~~

作者(Authors): 陈金辉;甘永健;崔国兵;邓懿祯;

DOI: 10.13346/j.mycosystema.210319

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