RNAi法分析广东虫草热激蛋白40 CgDnaJ05基因功能Function of the heat shock protein 40 gene CgDnaJ05 in Cordyceps guangdongensis analysed by RNAi
郝转;张成花;黄秋菊;黄浩;李泰辉;王刚正;邓旺秋;
摘要(Abstract)：
DnaJ蛋白(热激蛋白40)在机体生长发育和适应逆境环境过程中起着重要作用。以广东虫草GDGM30035为材料，克隆获得CgDnaJ05基因，CDS为2361bp，编码786个氨基酸，生物信息学分析结果表明CgDnaJ05蛋白含有DnaJ蛋白结构域，属于碱性、不稳定和亲水性蛋白质。以CgDnaJ05蛋白DnaJ保守结构域240 bp的反向互补片段为干扰片段，构建CgDnaJ05基因的双向启动子沉默载体；通过根癌农杆菌Agrobacteriumtumefaciens介导转化法侵染广东虫草菌丝，筛选获得9个相对稳定的CgDnaJ05沉默转化子。qRT-PCR分析表明，转化子CgDnaJ05相对表达量下调至野生型菌株的20%–82%；与野生菌丝相比，转化子菌丝生长速度显著下降，且CgDnaJ05下调越明显，菌丝减缓速度越显著，同时，转化子原基的形成受到显著抑制。研究表明：广东虫草CgDnaJ05基因明显影响广东虫草菌丝生长和原基形成，对广东虫草生长发育具有重要的调控作用。
关键词(KeyWords)： 广东虫草;CgDnaJ05;菌丝生长;原基形成
基金项目(Foundation): 国家自然科学基金(31900015);; 广东省科学院专项基金(2020GDASYL-20200103014);; 广州市科技计划(202102021051);; 广东省自然科学基金(2022A1515011083);; 陕西省教育厅科学研究计划(22JP022)~~
作者(Authors): 郝转;张成花;黄秋菊;黄浩;李泰辉;王刚正;邓旺秋;
DOI: 10.13346/j.mycosystema.220308
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