钱坤;武冬梅;王豪;孙一翡;司静;崔宝凯;

• 1:北京林业大学生态与自然保护学院微生物研究所
• 2:新疆农垦科学院生物技术研究所作物种质创新与基因资源利用兵团重点实验室

摘要(Abstract)：

灵芝是我国一类传统药用真菌的统称，具有极高的药用价值和经济价值。为了充分保护和利用该类野生药用真菌资源，本研究对采自广西壮族自治区南宁市的一株野生灵芝进行了菌株分离纯化培养，通过基于内转录间隔区(internal transcribed spacer,ITS)序列的分子生物学分析鉴定为四川灵芝Ganoderma sichuanense。以此为实验菌株，对该种的生物学特性和抗氧化活性进行了研究。探索了不同碳源、氮源、无机盐、pH、温度在固体培养条件下对野生四川灵芝菌丝生长速度的影响。对上述5因子进行单因子试验，从中筛选出4因子再进行正交试验。在试验范围内野生四川灵芝菌丝生长的最佳碳源为麦芽糖，氮源为牛肉膏，无机盐为KH_2PO_4，最适pH7.0，温度30℃。通过正交试验进一步优化，得到最佳因子组合为麦芽糖30.0g/L、牛肉膏5.0g/L、KH_2PO_4 1.0g/L、pH 6.0。针对液体培养过程中的多糖、三萜、多酚、抗坏血酸含量、超氧化物歧化酶活性及羟自由基清除能力变化进行了测定，发现该野生四川灵芝具有一定的抗氧化活性。

关键词(KeyWords)： 野生药用真菌;灵芝;生物学特性;抗氧化活性;驯化

Abstract:

Keywords:

基金项目(Foundation): 新疆生产建设兵团重点领域科技攻关计划项目(2021AB004);; 国家自然科学基金(U2003211);; 北京林业大学杰出青年人才培育计划项目(2019JQ03016)~~

作者(Authors): 钱坤;武冬梅;王豪;孙一翡;司静;崔宝凯;

DOI: 10.13346/j.mycosystema.210330

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