范绍斌;苏济钰;方甜;潘爱芳;何学友;樊改丽;王宗华;胡红莉;

• 1:福建农林大学植物保护学院
• 2:福州植物园
• 3:福建省林业科学研究院
• 4:厦门市绿化中心

摘要(Abstract)：

从福建多地采集了55份感染叶斑病的枫香植物病样,包含叶片、叶柄、枝条和树皮。通过分离纯化得到了12个类拟盘多毛孢属真菌菌株,综合形态特征以及3个基因(ITS、β-tubulin和tef1)的分子系统发育分析结果,分别鉴定为Neopestalotiopsis cocoes、N. chrysea、Pestalotiopsis neglecta和P. neolitseae,均为枫香上首次报道,其中N. cocoes在国内首次被发现。通过柯赫氏法则验证,发现N. cocoes能够侵染叶片、叶柄和枝条,N. chrysea能侵染叶片和枝条,P. neglecta能侵染叶柄和枝条,而P. neolitseae不能侵染枫香植物组织。

关键词(KeyWords)： 枫香树;叶部病害;类拟盘多毛孢真菌;形态类型;多基因系统发育分析

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31800008);; 枫香主要病虫害防治研究(闽林科[2016]3号);; 福建农林大学科技发展及科技创新专项基金项目(CXZX2016137);; 福建省住房和城乡建设系统2017年第一批科学技术项目(2017-K-90)~~

作者(Authors): 范绍斌;苏济钰;方甜;潘爱芳;何学友;樊改丽;王宗华;胡红莉;

DOI: 10.13346/j.mycosystema.210375

参考文献(References)：

• Ariyawansa HA,Hyde KD,2018.Additions to Pestalotiopsis in Taiwan.Mycosphere,9(5):999-1013
• Carbone I,Kohn LM,1999.A method for designing primer sets for speciation studies in filamentous ascomycetes.Mycologia,91(3):553-556
• Chen J,Hao X,Liu XF,Ma L,2020.First report of Pestalotiopsis neglecta causing black spot needle blight of Pinus sylvestris var.mongolica.in China.Plant Disease,104(5):1545
• Chen Q,Wu GB,Yang QM,Chen FH,2015.Pathogen identification and biological characteristics of wax apple soft rot disease.China Plant Protection,35(7):5-10 (in Chinese)
• Chi MQ,Wang Y,Ye JR,Zhang YF,2013.Morphological and molecular identification of Metasequoia red blight.Journal of Nanjing Forestry University (Natural Sciences Edition),37(5):75-80 (in Chinese)
• Gardes M,Bruns TD,1993.ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts.Molecular Ecology,2(2):113-118
• Ge QX,Chen YX,Xu T,2009.Flora fungorum sinicorum,Vol.38.Pestalotiopsis.Science Press,Beijing.1-272 (in Chinese)
• Glass NL,Donaldson GC,1995.Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes.Applied and Environmental Microbiology,61(4):1323-1330
• Hall TA,1999.Bio Edit:a user-friendly biological sequence alignment editor and analysis program for Windows95/98/NT.Nucleic Acids Symposium Series,41(41):95-98
• Hyde KD,Hongsanan S,Jeewon R,Bhat DJ,Mckenzie EHC,Jones EBG,Phookamsak R,Ariyawansa HA,Boonmee S,Zhao Q,Abdel-Aziz FA,Abdel-Wahab MA,Banmai S,Chomnunti P,Cui BK,Daranagama DA,Das K,Dayarathne MC,Silva NI,Zhu L,2016.Fungal diversity notes 367-490:taxonomic and phylogenetic contributions to fungal taxa.Fungal Diversity,80:1-270
• Larkin MA,Blackshields G,Brown NP,Chenna R,Mc Gettigan PA,Mc William H,Valentin F,Wallace IM,Wilm A,Lopez R,Thompson JD,Gibson TJ,Higgins DG,2007.Clustal W and Clustal X version 2.0.Bioinformatics,23:2947-2948
• Li CF,Liu L,Liu HY,2012.Investigation on diseases and insect pests of Liquidambar formosana and integrated control technology.Xiandai Horticulture,2012(10):155(in Chinese)
• Li DX,Zhao XZ,Wang Y,Lian SS,Ren YF,Chen Z,2018.Identification of the pathogen causing tea gray blight disease in Huishui County,Guizhou Province.Chinese Journal of Tropical Crops,39(9):1827-1833 (in Chinese)
• Lu B,Hyde KD,Ho WH,Tsui KM,Taylor JE,Wong KM,Yanna,Zhou D,2000.Checklist of Hong Kong fungi.Fungal Diversity Press,Hong Kong.1-207
• Maharachchikumbura SSN,Guo LD,Cai L,Chukeatirote E,Wu WP,Sun X,Crous PW,Bhat DJ,Mc Kenzie EHC,Bahkali AH,Hyde KD,2012.A multi-locus backbone tree for Pestalotiopsis,with a polyphasic characterization of 14 new species.Fungal Diversity,56:95-129
• Maharachchikumbura SSN,Hyde KD,Groenewald JZ,Xu J,Crous PW,2014.Pestalotiopsis revisited.Studies in Mycology,79:121-186
• Nguyen LT,Schmidt HA,von Haeseler A,Minh BQ,2015.IQ-TREE:a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies.Molecular Biology and Evolution,32(1):268-274
• Norphanphoun C,Jayawardena RS,Chen Y,Wen TC,Meepol W,Hyde KD,2019.Morphological and phylogenetic characterization of novel pestalotioid species associated with mangroves in Thailand.Mycosphere,10(1):531-578
• O’Donnell K,Kistler HC,Cigelnik E,Ploetz RC,1998.Multiple evolutionary origins of the fungus causing Panama disease of banana:concordant evidence from nuclear and mitochondrial gene genealogies.Proceedings of the National Academy of Sciences of the United States of America,95(5):2044-2049
• Pan AF,Ye GF,He XY,Lian QX,2018.Liquidambar formosana.China Forestry Publishing House,Beijing.1-60 (in Chinese)
• Ronquist F,Teslenko M,van der Mark P,Ayres DL,Darling A,H?hna S,Larget B,Liu L,Suchard MA,Huelsenbeck JP,2012.Mr Bayes 3.2:efficient Bayesian phylogenetic inference and model choice across a large model space.Systematic Biology,61(3):539-542
• Shi LB,Li Y,Fei NY,Fu JF,Yan XR,2016.First report of Neopestalotiopsis chrysea causing twig dieback of rabbiteye blueberry (Vaccinium ashei Reade) in China.Plant Disease,101(3):506
• Steyaert RL,1953.New and old species of Pestalotiopsis.Transactions of the British Mycological Society,36(2):81-89
• Wei JG,Xu T,Guo LD,Pan XH,2005.Endophytic Pestalotiopsis species from Southern China.Mycosystema,24(4):481-493 (in Chinese)
• White TJ,Bruns T,Lee S,Taylor J,1990.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics.PCR protocols:a guide to methods and applications.Academic Press,New York.315-322
• Wu F,Kong W,Zhang Y,Wu T,Wu XQ,2020.First report of leaf spot disease caused by Neopestalotiopsis chrysea on Carya illinoinensis in China.Plant Disease,105(1):221
• Zhang D,Gao F,Jakovli I,Zou H,Zhang J,Li WX,Wang GT,2020.Phylo Suite:an integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies.Molecular Ecology Resources,20(1):348-355
• Zhang XY,Li SJ,Wang L,Yang YL,2019.Pathogen identification of gray leaf spot on Camellia japonica and screening of the fungicides.Plant Protection,45(4):209-215,242 (in Chinese)
• Zhou L,Li J,Chen F,Chen J,Ye J,2019.First report of shoot blight on Cryptomeria japonica caused by Pestalotiopsis neglecta in China.Plant Disease,103(8):2140
• Zhu YY,Su JY,Pan AF,He XY,Hu HL,2019.Colletotrichum species responsible for leaf anthracnose of Liquidambar formosana Hance in Fujian.Chinese Journal of Tropical Crops,40(11):2197-2204 (in Chinese)
• 陈前,吴光斌,杨秋明,陈发河,2015.莲雾软腐病病原菌鉴定及其生物学特性研究.中国植保导刊,35(7):5-10
• 匙明强,王焱,叶建仁,张岳峰,2013.水杉赤枯病病原形态及分子鉴定.南京林业大学学报(自然科学版),37(5):75-80
• 葛起新,陈育新,徐同,2009.中国真菌志.第38卷.拟盘多毛孢属.北京:科学出版社.1-235
• 李超飞,刘磊,刘怀宇,2012.枫香的病虫害调查及综合防治技术.现代园艺,2012(10):155
• 李冬雪,赵晓珍,王勇,练珊珊,任亚峰,陈卓,2018.贵州惠水县茶轮斑病病原菌的鉴定.热带作物学报,39(9):1827-1833
• 潘爱芳,叶功富,何学友,连巧霞,2018.枫香.北京:中国林业出版社.1-60
• 韦继光,徐同,郭良栋,潘秀湖,2005.中国南方内生拟盘多毛孢.菌物学报,24(4):481-493
• 张晓勇,李树江,王亮,杨友联,2019.山茶灰斑病病原菌鉴定及防治药剂初步筛选.植物保护,45(4):209-215,242
• 朱仰艳,苏锦钰,潘爱芳,何学友,胡红莉,2019.福建枫香炭疽病病原的种类鉴定.热带作物学报,40(11):2197-2204