胡永乐;张传海;林崇展;尤永芳;黄薇;

• 1:武夷学院生态与资源工程学院福建省生态产业绿色技术重点实验室

摘要(Abstract)：

为提高蛹虫草液体发酵胞外多糖含量和菌丝体生物量,以厚朴为药性基质,对蛹虫草进行双向液体发酵。在单因素实验的基础上,应用Box-Behnken实验设计,对发酵过程关键影响因素进行优化。结果表明,在厚朴添加量5g、接种量15.5m L、发酵温度25℃的条件下发酵9d,蛹虫草双向液体发酵产物中胞外多糖含量为3.11mg/m L,菌丝体生物量为18.81mg/m L。各发酵因素中,发酵液胞外多糖含量受接种量影响最大,菌丝体生物量则主要受发酵温度影响。优化所得发酵工艺可行性高、周期短、生产过程可控,为进一步提高人工培育蛹虫草质量、增加其关键活性产物的产量提供了参考。

关键词(KeyWords)： 蛹虫草;厚朴;响应面优化;胞外多糖;菌丝体生物量

Abstract:

Keywords:

基金项目(Foundation): 福建省自然科学基金(2019J01827);; 福建省生态产业绿色技术重点实验室开放课题基金(WYKF2018-6);; 国家级大学生创新创业训练计划项目(201810397005)~~

作者(Authors): 胡永乐;张传海;林崇展;尤永芳;黄薇;

DOI: 10.13346/j.mycosystema.190329

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