程池露;李传华;熊海宽;冯娜;张劲松;

• 1:上海海洋大学食品学院
• 2:上海市农业科学院食用菌研究所农业部南方食用菌资源利用重点实验室国家食用菌工程技术研究中心国家食用菌加工技术研发分中心上海市农业遗传育种重点开放实验室
• 3:永仁野森达菌业有限公司

摘要(Abstract)：

本研究建立了一种固相萃取-超高效液相色谱-串联质谱的检测方法，用于检测新鲜块菌子实体中α-雄烷醇(5α-雄甾-16-烯-3α-醇)的含量。新鲜块菌样品经无水乙醇提取，Qasis HLB柱萃取富集后，采用超高效液相色谱-串联质谱进行分析定量。方法学验证结果表明该方法的回收率为88.49%–92.22%；检出限为0.120 9 ng/mL，定量限为0.398 9 ng/mL。该方法简便、精确，适用于新鲜块菌中α-雄烷醇含量的测定。

关键词(KeyWords)： 块菌;α-雄烷醇;超高效液相色谱-串联质谱

Abstract:

Keywords:

基金项目(Foundation): 云南省张劲松专家工作站(20210SAF150009)~~

作者(Authors): 程池露;李传华;熊海宽;冯娜;张劲松;

DOI: 10.13346/j.mycosystema.210454

参考文献(References)：

• Brooksbank B,Haslewood G,1961.The estimation of androst-16-en-3alpha-ol in human urine.Partial synthesis of androstenol and of its beta-glucosiduronic acid.Biochemical Journal,80(3):488
• Chen J,Li SC,Li JM,Yan B,Liu PG,2018.Distribution,biological diversity,and conservation of Chinese black truffle.Scientia Sinica Vitae,48(3):319-331 (in Chinese)
• Claus R,Hoppen HO,Karg H,1981.The secret of truffles:a steroidal pheromone?Experientia,37(11):1178-1179
• David B,Vivien W,1986.Effects of androstenol on human sexual arousal.Biological Psychology,22(2):141-147
• Fan L,2021.Current taxonomic and geographic situation on Asian black truffles.Journal of Fungal Research,19(1):6-11 (in Chinese)
• Huang ZG,Wei CH,Luo HB,Liu YM,Deng J,2014.Study on extraction and detection ofα-androstenol in truffle.Advanced Materials Research,881-883:781-784
• Kazuyuki S,Masayo M,Tosihya F,Dai M,Fukuko K,2000.Effects of 5alpha-androst-16-en-3alpha-ol on the pulsatile secretion of luteinizing hormone in human females.Chemical Senses,21(4):465-467
• Li SC,Qiao P,Liu SS,Chen J,Guo SX,2017.Research progress in the molecular systematics and symbiotic mechanism of Tuber (Tuberaceae,Ascomycota).Mycosystema,36(2):131-144 (in Chinese)
• Li XF,Yang B,Zhang HQ,2018.Evaluation of nutritional value of natural black truffle grown in Yunnan.Food Research and Development,39(11):157-161 (in Chinese)
• Ning YC,2018.Structural identification of organic compounds and organic spectroscopy.4th ed.Science Press,Beijing.266-316 (in Chinese)
• Payen T,Murat-Furminieux C,Bonito G,2014.Truffle phylogenomics:new insights into truffle evolution and truffle life cycle.Advances in Botanical Research,70:211-234
• Prelog V,Ruzicka L,1944.Untersuchungenüber Organextrakte.(5.Mitteilung).über zwei moschusartig riechende Steroide aus Schweinetestes-Extrakten.Helvetica Chimica Acta,27(1):61-66
• Richard S,Nayuf V,Nina K,Marta CO,Jean-Pierre S,Ivo F,Petr K,2012.Truffle volatiles:from chemical ecology to aroma biosynthesis.New Phytologist,189(3):688-699
• Schmidberger PC,Schieberle P,2017.Characterization of the key aroma compounds in white alba truffle (Tuber magnatum Pico) and burgundy truffle (Tuber uncinatum)by means of the sensomics approach.Journal of Agricultural and Food Chemistry,65(42):9287-9296
• Wang G,Li YY,Li DS,Tang YJ,2008.Determination of5α-androst-16-en-3α-ol in truffle fermentation broth by solid-phase extraction coupled with gas chromatography-flame ionization detector/electron impact mass spectrometry.Journal of Chromatography B,870(2):209-215
• Wu Z,Meenu M,Xu B,2021.Nutritional value and antioxidant activity of Chinese black truffle (Tuber indicum) grown in different geographical regions in China.LWT-Food Science and Technology,135:110226
• 陈娟，李淑超，李佳梅，阎波，刘培贵，2018.中国“黑块菌”(黑松露)新分布区确认及物种多样性保护.中国科学：生命科学，48(3):319-331
• 范黎，2021.亚洲黑松露(块菌)系统分类和地理分布研究进展.菌物研究，19(1):6-11
• 李淑超，乔鹏，刘思思，陈娟，郭顺星，2017.块菌属分子系统学及菌根共生机制研究进展.菌物学报，36(2):131-144
• 李晓凤，杨兵，张华琦，2018.云南野生黑松露营养价值评定.食品研究与开发，39(11):157-161
• 宁永成，2018.有机化合物结构鉴定与有机波谱学.第四版.北京：科学出版社.266-316