耿荣;耿增超;黄建;和文祥;侯琳;佘雕;韩其晟;龙东风;

• 1:西北农林科技大学资源环境学院农业部西北植物营养与农业环境重点实验室
• 2:西北农林科技大学林学院
• 3:西北农林科技大学水土保持研究所

摘要(Abstract)：

以秦岭辛家山林区锐齿栎为研究对象,通过野外调查结合形态学和分子生物学方法,观察和鉴定与其共生的外生菌根真菌。结果确定了3种子囊菌和48种担子菌,分属于10科14属,其中毛革菌属Tomentella是优势类群,丝伞盖属1 Inocybe 1、Russula persicina、毛革菌属1 Tomentella 1、毛革菌属2 Tomentella 2、块菌属Tuber、绒盖牛肝菌属Xerocomus是常见种,其余都为稀有种。两样地锐齿栎外生菌根真菌除丰富度指数外,Simpson指数、Shannon指数以及Pielou均匀度指数差异不显著;Jaccard相似性指数和Sorenson相似性指数分别为0.1154和0.2069。

关键词(KeyWords)： 锐齿栎;外生菌根真菌;多样性;形态学;分子生物学;菌根侵染率

Abstract:

Keywords:

基金项目(Foundation): 国家林业公益性行业科研专项(201304307)~~

作者(Authors): 耿荣;耿增超;黄建;和文祥;侯琳;佘雕;韩其晟;龙东风;

DOI: 10.13346/j.mycosystema.150122

参考文献(References)：

• Altschul SF,Madden TL,Schaffer AA,Zhang J,Zhang Z,Miller W,Lipman DJ,1997.Gapped BLAST and PSI-BLAST:a new generation of protein batabase search programs.Nucleic Acids Research,25(17):3389-3402
• Bai SL,Liu Y,Zhou J,Dong Z,Fan R,2006.Resources investigation and ecological study on ectomycorrhizal fungi in Daqingshan Mountains,Inner Mongolia.Acta Ecologica Sinica,26(3):837-841(in Chinese)
• Cui L,Mu LQ,2014.The morphological and anatomical characteristics of ectomycorrhizae colonizing T ilia amurensis Rupr.in Heilongjiang Province.Chinese Journal of Ecology,3(9):2490-2500(in Chinese)
• Fan YJ,Yan W,2013.Morphological type and molecular identification of ectomycorrhizae on Betula platyphylla in Inner Mongolia Area.Acta Botanica Boreali-Occidentalis Sinica,33(11):2209-2215(in Chinese)
• Fan YJ,Yan W,Wang LY,2011.Morphological type and molecular identification of ectomycorrhizae on Picea crassifolia in Helan Mountain.Scientia Silvae Sinicae,47(6):108-113(in Chinese)
• Futai K,Taniguchi T,Kataoka R,2008.Ectomycorrhizae and their importance in forest ecosystems.In:Mycorrhizae:sustainable agriculture and forestry.Springer,Netherlands.241-285
• Gardes M,Bruns TD,1993.ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts.Molecular Ecology,2(2):113-118
• He SC,1991.A preliminary study on the resources of forest ectotrophic fungi and their ecological distribution from Guizhou Province,China.Guizhou Science,9(1):51-58(in Chinese)
• He YH,Zhou GY,Wang SJ,Li H,2014.Fungal diversity in Cunninghamia lanceolata plantation soil.Acta Ecologica Sinica,34(10):2725-2736(in Chinese)
• Horton TR,Bruns TD,2001.The molecular revolution in ectomycorrhizal ecology:peeking into the black-box.Molecular Ecology,10:1855-1871
• Huang J,Nara K,Lian CL,Zong K,Peng KJ,Xue SG,Shen ZG,2012.Ectomycorrhizal fungal communities associated with Masson pine(Pinus massoniana Lamb.)in Pb-Zn mine sites of central south China.Mycorrhiza,22(8):589-602
• Huang JG,Lapeyrie F,1996.Ability of ectomycorrhizal fungus Laccaria bicolor S238N to increase the growth of Douglas fir seedlings and their phosphorus and potassium uptake.Pedosphere,6(3):217-224
• Koele N,Turpault MP,Hildebrand EE,Uroz S,Frey-Klett P,2009.Interactions between mycorrhizal fungi and mycorrhizosphere bacteria during mineral weathering:budget analysis and bacterial quantification.Soil Biology and Biochemistry,41(9):1935-1942
• Krpata D,Peintner U,Langer I,Fitz WJ,Schweiger P,2008.Ectomycorrhizal communities associated with Populus tremula growing on a heavy metal contaminated site.Mycological Research,112(9):1069-1079
• Lian C,Hogetsu T,Matsushita N,Guerin-Laguette A,Suzuki K,Yamada A,2003.Development of microsatellite markers from an ectomycorrhizal fungus,Tricholoma matsutake,by an ISSR-suppression-PCR method.Mycorrhiza,13(1):27-31
• Luo GT,Wang JX,Yuan W,2007.Investigation of the Macrofungi of ectotrophicmycorrhiza of Pinus massoniana in Kaili City,Guizhou Province.Edible Fungi of China,26(2):12-14(in Chinese)
• Meng FR,Shao JW,2001.The ecological distribution of ecto-mycorrhizal fungi in main coniferous forests in Northeast China.Mycosystema,20(3):413-419(in Chinese)
• Nagaike T,Hayashi A,Kubo M,Takahashi K,Abe M,Arai N,2006.Changes in plant species diversity over 5 years in Larix kaempferi plantations and abandoned coppice forests in central Japan.Forest Ecology and Management,236:278-285
• Nieto MP,Carbone SS,2009.Characterization of juvenile maritime pine(Pinus pinaster Ait.)ectomycorrhizal fungal community using morphotyping,direct sequencing and fruitbodies sampling.Mycorrhiza,19(2):91-98
• Peay KG,Garbelotto M,Bruns TD,2009.Spore heat resistance plays an important role in disturbance-mediated assemblage shift of ectomycorrhizal fungi colonizing Pinus muricata seedlings.Journal of Ecology,97(3):537-547
• Peng YX,Song M,Pedruzzi I,Li P,Yu JG,2015.K-release and weathering of muscovite by Cenococcum geophilum.Acta Microbiologica Sinica,55(3):282-291(in Chinese)
• Song FQ,Liu YK,Du CM,Yang FS,2011.Diversity of ectomycorrhizal macro-fungi at Xiao Hinggan mountain and medium optimization for Suillus grevillei using response surface methodology.Journal of Natural Resources,26(3):440-449(in Chinese)
• Suo AN,Ju TZ,Zhang JH,GE JP,2005.Biomass structure of Quercus aliena var.acuteserrata community on Mt.Xiaolongshan in Gansu.Chinese Journal of Ecology,24(44):377-381(in Chinese)
• Tedersoo L,Jairus T,Horton BM,Abarenkov K,Suvi T,Saar I,Koljalg U,2008.Strong host preference of ectomycorrhizal fungi in a Tasmanian wet sclerophyll forest as revealed by DNA barcoding and taxon-specific primers.New Phytologist,180(2):479-490
• Tedersoo L,K?ljalg U,Hallenberg N,Larsson KH,2003.Fine scale distribution of ectomycorrhizal fungi and roots across substrate layers including coarse woody debris in a mixed forest.New Phytologist,159:153-165
• Wallander H,Wickman T,1999.Biotite and microcline as potassium sources in ectomycorrhizal and non-mycorrhizal Pinus sylvestris seedlings.Mycorrhiza,9(1):25-32
• Wang H,Wang YP,Lin Q,Shi JY,Chen YX,2006.Analysis of rhizosphere microbial community structures in heavy metal-contaminated soils using PCR and denaturation gradient gel electrophoresis(DGGE).Journal of Agro-Environment Science,25(4):903-907(in Chinese)
• Wang Q,2013.Ectomycorrhizal community composition of Quercus mongolica.Journal of Liaoning Forestry Science&Technology,2013(3):6-9(in Chinese)
• Wang R,Liu CG,Xu J,2013.The separation and cultivation of ectomycorrhizal fungi.Edible Fungi of China,32(1):4-7(in Chinese)
• Wei J,Yan W,2015.A checklist of ectomycorrhizal fungi of Chinese pine.Northern Horticulture,3:126-130(in Chinese)
• White TJ,Bruns TD,Lee S,Taylor J,1990.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics.In:Innis M,Gelfand D,Sninsky J,White T(eds.)PCR protocols:a guide to methods and applications.Academic Press,San Diego.315-322
• Wu CH,Wang JR,Yang JX,Wu GH,2001.A study on ectomycorrhizae and mycorrhiza fungi in Taibai Mountain Nature Reserve.Journal of Northwest Agriculture and Forestry University(Natural Science),29(2):56-60(in Chinese)
• Wu K,2003.Research on ectomycorrhizal fungi of Picea koraiensis.Master Thesis,Northeast Forestry University,Harbin.8-9(in Chinese)
• Yan XF,Wang Q,2002.Ectomycorrhizal formation in seedlings of Quercus liaotungensis.Acta Phytoecologica Sinica,26(1):64-68(in Chinese)
• Yin CM,Wang GS,Li YY,Chen XS,Wu SJ,Mao ZQ,2014.Assessment of fungal diversity in apple replanted orchard soils by T-RFLP analysis.Acta Ecologica Sinica,34(4):837-846(in Chinese)
• Yu FQ,Xiao YQ,Liu PG,2007.Spatiotemporal distribution of ectomycorrhizal fungi in Pinus yunnanensis forests.Acta Ecologica Sinica,27(6):2325-2333(in Chinese)
• Zhang RQ,Tang M,Zhang HH,Du XG,2011.A preliminary investigation on ectomycorrhizal fungi in Huoditang forest region of Qinling Mountains.Journal of Qingdao Agricultural University(Natural Science),28(3):168-173(in Chinese)
• Zhao Z,Ma KX,Duan AA,1993.Research on types and ecological characteristics of ectomycorrhiza of Populus tomentosa.Scientia Silvae Sinicae,29(1):12-18(in Chinese)
• Zheng YK,Ren D,Lu SE,Li XL,Zhang XP,Gu YF,2014.Diversity and community structure of endophytic actinomycetes of Pteris vittata in a Pb-Zn mining area.Acta Scientiae Circumstantiae,34(11):2832-2838(in Chinese)
• Zhu TH,Zhang J,Hu TX,Li XW,Liu YG,2001.The study on ectomycorrhizal fungi associated with Eucalyptus in Sichuan.Journal of Sichuan Agricultural University,19(2):137-140(in Chinese)
• 白淑兰,刘勇,周晶,董智,樊荣,2006.大青山外生菌根真菌资源与生态研究.生态学报,26(3):837-841
• 崔磊,穆立蔷,2014.黑龙江省紫椴典型分布区外生菌根形态与解剖特征.生态学杂志,33(9):2490-2500
• 樊永军,闫伟,2013.内蒙古地区白桦外生菌根形态类型及分子鉴定.西北植物学报,33(11):2209-2215
• 樊永军,闫伟,王黎元,2011.贺兰山地区青海云杉外生菌根的形态.林业科学,47(6):108-113
• 何绍昌,1991.贵州林木外生菌根菌种类及生态、分布的初步研究.贵州科学,9(1):51-58
• 何苑皞,周国英,王圣洁,李河,2014.杉木人工林土壤真菌遗传多样性.生态学报,34(10):2725-2736
• 罗国涛,王健祥,袁玮,2007.凯里市马尾松外生菌根菌的调查.中国食用菌,26(2):12-14
• 孟繁荣,邵景文,2001.东北主要林区针叶林下外生菌根真菌及生态分布.菌物系统,20(3):413-419
• 彭云湘,宋淼,Israel Pedruzzi,李平,于建国,2015.土生空团菌对白云母的风化作用及解钾特性.微生物学报,55(3):282-291
• 宋福强,刘远开,杜春梅,杨峰山,2011.小兴安岭红松外生菌根真菌资源及响应面法优化Suillus grevillei培养基.自然资源学报,26(3):440-449
• 索安宁,巨天珍,张俊华,葛剑平,2005.甘肃小陇山锐齿栎群落生物量动态研究.生态学杂志,24(44):377-381
• 王慧,王远鹏,林琦,施积炎,陈英旭,2006.应用PCR-DGGE研究铜冶炼厂附近根际土壤微生物生态变化.农业环境科学学报,25(4):903-907
• 王琴,2013.蒙古栎外生菌根真菌多样性研究.辽宁林业科技,3:6-9
• 王冉,刘朝贵,须建,2013.外生菌根菌的分离与培养的研究进展.中国食用菌,32(1):4-7
• 魏杰,闫伟,2015.油松外生菌根真菌名录.北方园艺,3:126-130
• 吴重华,王吉忍,杨俊秀,吴国华,2001.太白山自然保护区外生菌根及菌根真菌调查研究.西北农林科技大学学报(自然科学版),29(2):56-60
• 吴克,2003.云杉外生菌根菌的研究.东北林业大学硕士论文,哈尔滨.8-9
• 阎秀峰,王琴,2002.辽东栎幼苗的外生菌根合成.植物生态学报,26(1):64-68
• 尹承苗,王功帅,李园园,陈学森,吴树敬,毛志泉,2014.连作苹果园土壤真菌的T-RFLP分析.生态学报,34(4):837-846
• 于富强,肖月芹,刘培贵,2007.云南松(Pinus yunnanensis)林外生菌根真菌的时空分布.生态学报,27(6):2325-2333
• 张茹琴,唐明,张海涵,杜小刚,2011.秦岭火地塘林区外生菌根真菌的初步调查.青岛农业大学学报(自然科学版),28(3):168-173
• 赵忠,马刊欣,段安安,1993.毛白杨外生菌根类型及其生态学特性的研究.林业科学,29(1):12-18
• 郑有坤,任丹,卢圣鄂,李小林,张小平,辜运富,2014.铅锌矿区蜈蚣草内生放线菌的多样性及群落结构.环境科学学报,34(11):2832-2838
• 朱天辉,张健,胡庭兴,李贤伟,刘应高,2001.四川桉树外生菌根真菌的研究.四川农业大学学报,19(2):137-140