李芳;张珏锋;钟海英;陈建明;

• 1:浙江省农业科学院植物保护与微生物研究所

摘要(Abstract)：

球孢白僵菌作为丝孢类昆虫病原真菌，已广泛应用于农林害虫的生物防治，但是田间多变的环境影响了真菌制剂的效能。此外，真菌侵染宿主后，宿主体内的环境也影响真菌的增殖和侵染速度。为研究球孢白僵菌对环境中酸碱度及微量元素的平衡能力，本研究初步探讨了锌离子响应转录激活因子ZafA与真菌生长繁殖、抗逆能力、毒力以及锌离子利用的关系。结果表明，敲除zafA削弱了真菌生长繁殖和孢子萌发的能力，增加了菌株对氧化、高渗、孢壁干扰剂以及紫外胁迫的敏感性，杀虫毒力显著下降，并抑制了相关性状基因的表达水平。基因敲除菌株无法在锌离子缺失的条件下生长，且zafA基因和锌离子转运蛋白编码基因zrf1–8的表达水平会受到酸碱度以及锌离子浓度的影响。由此可见，ZafA不仅直接影响球孢白僵菌利用锌离子的能力，还与球孢白僵菌抗逆能力和毒力密切相关，本研究为提高生防真菌环境适应性和发挥毒力提供新的依据。

关键词(KeyWords)： 球孢白僵菌;锌离子响应转录激活因子;锌离子转运蛋白;抗逆力;毒力

Abstract:

Keywords:

基金项目(Foundation): 浙江省自然科学基金(LQ19C140003);; 国家自然科学基金(32001963);; 财政部和农业部现代农业产业技术体系建设专项资金(CARS-24-G-07);; 黄岩区农业绿色发展先行先试支撑体系建设服务项目(HY202002);; 浙江省重点研发计划(2019C0203003)~~

作者(Authors): 李芳;张珏锋;钟海英;陈建明;

DOI: 10.13346/j.mycosystema.210337

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