王妮;谢映平;樊金华;

• 1:山西大学生命科学学院
• 2:山西农业大学林学院

摘要(Abstract)：

从太原市白蜡树上采集的自然染病死亡的桑白盾蚧Pseudaulacaspis pentagona虫尸上分离获得一株病原真菌,菌株号为TYL001。经过形态观察和分子鉴定,该菌株为Simplicillium lanosoniveum。用该菌株5种浓度的孢子悬浮液(1.0×10⁶孢子/m L、5.0×106孢子/m L、5.0×10⁶孢子/m L、1.0×106孢子/m L、1.0×10⁷孢子/m L、5.0×107孢子/m L、5.0×10⁷孢子/m L、1.0×107孢子/m L、1.0×10⁸孢子/m L)感染桑白盾蚧雌成虫,感染6d后,孢子悬浮液对桑白盾蚧的感染率分别为80.00%、83.33%、86.67%、90.00%、93.33%,说明该菌株对桑白盾蚧具有较强的感染力,且有浓度效应;感染症状出现的高峰期在72–120h之间,且孢子浓度越大,感染症状出现的越早;5种孢子浓度的感染中时IT50依次为106.95h、97.00h、91.46h、87.73h、79.38h,说明感染致病所用时间也具有浓度效应。本研究为生物防治桑白盾蚧提供了新菌种。

关键词(KeyWords)： 桑白盾蚧;病原真菌;Simplicillium lanosoniveum;分离鉴定;感染

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(30671693)~~

作者(Authors): 王妮;谢映平;樊金华;

DOI: 10.13346/j.mycosystema.150182

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