冯万艳,赵燕珍,谭健晖,杨章旗,孙学广

• 1:贵州大学贵州省森林资源与环境研究中心/林学院
• 2:广西壮族自治区林业科学研究院

摘要(Abstract)：

对菌根共生机制的研究是对其进行应用的前提,到目前为止,绝大多数外生菌根(ectomycorrhiza,ECM)的建立过程尚不明晰,在一定程度上限制了这些ECM真菌在林业中的应用。本研究以我国南方地区主栽树种之一——马尾松Pinus massoniana和其林下优势ECM真菌——粘盖乳牛肝菌Suillus bovinus为材料,在无菌条件下研究两者菌根共生体形成过程的形态学特征。结果表明马尾松与粘盖乳牛肝菌的共生过程分为2个阶段:(1)预共生阶段,即物理接触之前,粘盖乳牛肝菌可通过释放挥发物和分泌物促进马尾松根系伸长和分枝;(2)共生阶段,又可分为3个时期。接种后第4天,粘盖乳牛肝菌菌丝体开始与马尾松根系接触并形成附着胞进入接触期;第7天菌丝开始侵入根系内部,侵入期开始;第28天菌套和哈氏网形成,即菌套和哈氏网形成期,该时期菌根化根尖开始膨大,随后继续发育至二叉分枝状菌根形成。在发育顺序方面,哈氏网与菌套同步发育,但哈氏网成形早于菌套。以上结果可对后续ECM共生机制的深入研究及马尾松高效菌根化育苗技术的开发提供参考。

关键词(KeyWords)： 外生菌根;马尾松;粘盖乳牛肝菌;共生体形成;形态特征

Abstract:

Keywords:

基金项目(Foundation): 广西创新驱动发展专项（AA17204087-4）;; 贵州省一流学科建设项目（GNYL[2017]007）;; 贵州省科学技术基金（[2015]2039）;; 贵州省教育厅自然科学研究项目([2015]442)~~

作者(Author): 冯万艳,赵燕珍,谭健晖,杨章旗,孙学广

DOI: 10.13346/j.mycosystema.190306

参考文献(References)：

• Baptista P,Martins A,Pais MS,Tavares RM,Lino-Neto T,2007.Involvement of reactive oxygen species during early stages of ectomycorrhiza establishment between Castanea sativa and Pisolithus tinctorius.Mycorrhiza,17(3):185-193
• Brunner I,1991.Comparative studies on ectomycorrhizae synthesized with various in vitro techniques using Picea abies and two Hebeloma species.Trees,5(2):90-94
• Chilvers GA,Douglass PA,Lapeyrie FF,1986.Apaper-sandwich technique for rapid synthesis of ectomycorrhizas.New Phytologist,103(2):397-402
• Ditengou FA,Béguiristain T,Lapeyrie F,2000.Root hair elongation is inhibited by hypaphorine,the indole alkaloid from the ectomycorrhizal fungus Pisolithus tinctorius,and restored by indole-3-acetic acid.Planta,211(5):722-728
• Ditengou FA,Müller A,Rosenkranz M,Felten J,Lasok H,Doorn MM,LeguéV,Palme K,Schnitzler JP,Polle A,2015.Volatile signalling by sesquiterpenes from ectomycorrhizal fungi reprogrammes root architecture.Nature Communications,6:6279
• Duplessis S,Courty P,Tagu D,Martin F,2005.Transcript patterns associated with ectomycorrhiza development in Eucalyptus globulus and Pisolithus microcarpus.New Phytologist,165:599-611
• Felten J,Kohler A,Morin E,Bhalerao RP,Palme K,Martin F,Ditengou FA,LeguéV,2009.The ectomycorrhizal fungus Laccaria bicolor stimulates lateral root formation in Poplar and Arabidopsis through auxin transport and signaling.Plant Physiology,151(4):1991-2005
• Felten J,LeguéV,Ditengou FA,2010.Lateral root stimulation in the early interaction between Arabidopsis thaliana and the ectomycorrhizal fungus Laccaria bicolor:is fungal auxin the trigger?Plant Signaling&Behavior,5(7):864
• Felten J,Martin F,LeguéV,2012.Signalling in ectomycorrhizal symbiosis.In:Signaling and communication in plant symbiosis.Springer,Berlin,Heidelberg.123-142
• Feng WY,Hu D,Feng JW,Chen YQ,Guo QQ,Sun XG,2019.Characterization of two edible mycorrhizal species of Suillus in masson pine forest.Mycosystema,38(6):792-801(in Chinese)
• Fusconi A,1983.The development of the fungal sheath on Cistus incanus short roots.Canadian Journal of Botany,61(10):2546-2553
• Grellier B,Letouze R,Strullu DG,1984.Micropropagation of birch and mycorrhizal formation in vitro.New Phytologist,97(4):591-599
• Guerin-Laguette A,Vaario LM,Gillet WM,Lapeyrie F,Matsushita N,Suzukp K,2000.Rapid in vitro ectomycorrhizal infection on Pinus densiflora roots by Tricholoma matsutake.Mycoscience,41:389-393
• Gupta PK,Durzan DJ,1985.Shoot multiplication from mature trees of Douglas-fir(Pseudotsuga menziesii)and sugar pine(Pinus lambertiana).Plant Cell Reports,4(4):177-179
• Horan DP,Chilvers GA,Lapeyrie FF,1988.Time sequence of the infection process Eucalypt ectomycorrhizas.New Phytologist,109(4):451-458
• Lagrange H,Jay-Allgmand C,Lapeyrie F,2001.Rutin,the phenolglycoside from eucalyptus root exudates,stimulates Pisolithus hyphal growth at picomolar concentrations.New Phytologist,149(2):349-355
• Liu RJ,Chen YL,2007.Mycorrhizology.Science Press,Beijing.125-127(in Chinese)
• Melville LH,Massicotte HB,Peterson RL,1987.Ontogeny of early stages of ectomycorrhizae synthesized between Dryas integrifolia and Hebeloma cylindrosporum.Botanical Gazette,148(3):332-341
• Nylund J,Unestam T,1982.Structure and physiology of ectomycorrhizae.I.The process of mycorrhiza formation in Norway spruce in vitro.New Phytologist,91(1):63-79
• Plett JM,Daguerre Y,Wittulsky S,Vayssières A,Deveau A,Melton SJ,Kohler A,Morrell-Falvey JL,Brun A,Veneault-Fourrey C,Martin F,2014.Effector MiSSP7 of the mutualistic fungus Laccaria bicolor stabilizes the Populus JAZ6 protein and represses jasmonic acid(JA)responsive genes.Proceedings of the National Academy of Sciences of the United States of America,111(22):8299-8304
• Plett JM,Yin H,Mewalal R,Hu R,Li T,Ranjan P,Jawdy S,De Paoli HC,Butler G,Burch-Smith TM,Guo H,Chen CJ,Kohler A,Anderson IC,LabbéJL,Martin F,Tuskan GA,Yang XH,2017.Populus trichocarpa encodes small,effector like secreted proteins that are highly induced during mutualistic symbiosis.Scientific Reports,7(1):382
• Sebastiana M,Vieira B,Lino-Neto T,Monteiro F,Figueiredo A,Sousa L,Pais MS Tavares R,Paulo OS,2014.Oak root response to ectomycorrhizal symbiosis establishment:RNA-Seq derived transcript identification and expression profiling.PLoS One,9(5):e98376
• Shu XQ,2017.Study on tissue culture and genetic transformation of Pinus massoniana.Master Thesis,Nanjing Forestry University,Nanjing.1-41
• Smith SE,Read DJ,2008.Mycorrhizal symbiosis.3rd eds.Academic Press,London.192-268
• Sun X,Bonfante P,Tang M,2015.Effects of volatiles versus exudates released by germinating spores of Gigaspora margarita on lateral root formation.Plant Physiology and Biochemistry,97:1-10
• Sun XG,Feng WY,Li M,Shi J,Ding GJ,2019.Phenology and cultivation of Suillus bovinus,an edible mycorrhizal fungus,in a Pinus massoniana plantation.Canadian Journal of Forest Research,49:960-968
• Tranvan H,Habricot Y,Jeannette E,Gay G,Sotta B,2000.Dynamics of symbiotic establishment between an IAA-overproducing mutant of the ectomycorrhizal fungus Hebeloma cylindrosporum and Pinus pinaster.Tree Physiology,20:123-129
• Wang JG,Zheng R,Bai SL,Liu S,Yan W,2012.Responses of ectomycorrhiza to drought stress:a review.Chinese Journal of Ecology,31(6):1571-1576(in Chinese)
• Wu XQ,Sun MQ,2006.Mycorrhizal formation between seven ectomycorrhizal fungi and seedlings of three pines species.Acta Ecologica Sinica,26(12):4186-4191(in Chinese)
• Xue Y,2006.Study on technology about tissue culture rapid propagation of the masson pine.Master Thesis,Guangxi University,Nanning.19-25(in Chinese)
• Yu JY,2007.Infecting micromechanism of ectomycorrhizal fungi to Picea koraiensis.Master Thesis,Northeast Forestry University,Harbin.27-34(in Chinese)
• Zhu LH,Wu XQ,Qu HY,Ji J,Ye JR,2010.Micropropagation of Pinus massoniana and mycorrhiza formation in vitro.Plant Cell,Tissue and Organ Culture(PCTOC),102(1):121-128
• 冯万艳，胡丹，冯婧玮，陈雨倩，郭其强，孙学广，2019.两种乳牛肝菌属菌根食用菌与马尾松的共生特征.菌物学报，38(6):792-801
• 刘润进，陈应龙，2007.菌根学.北京：科学出版社.125-127
• 殳晓强，2017.马尾松组织培养与遗传转化的研究.南京林业大学硕士论文，南京.18-28
• 王琚钢，峥嵘，白淑兰，刘声，闫伟，2012.外生菌根对干旱胁迫的响应.生态学杂志，31(6):1571-1576
• 吴小芹，孙民琴，2006.七株外生菌根真菌与三种松苗菌根的形成能力.生态学报，26(12):4186-4191
• 薛鹰，2006.马尾松组织培养快速繁殖技术的研究.广西大学硕士论文，南宁.19-25
• 余江勇，2007.外生菌根菌侵染红皮云杉微观机制的研究.东北林业大学硕士论文，哈尔滨.27-34