捕食线虫真菌少孢节丛孢Arthrobotrys oligospora线粒体基因组的异质性Evident presence of heteroplasmy in the mitochondrial genomes of the nematode-trapping fungus Arthrobotrys oligospora
李烁烁;王达;房美玲;张颖;
摘要(Abstract)：
捕食线虫真菌少孢节丛孢Arthrobotrys oligospora是子囊菌中的一个食肉性丝状真菌，其菌丝体可变态为各种结构精巧的捕食器官用于捕食线虫，在生物防治和进化上极具研究潜力。线粒体基因细胞色素c氧化酶亚基Ⅰ基因(COX1)在真菌物种鉴定中的效果饱受争议。本研究对A. oligospora的不同单孢培养的菌株进行了COX1基因的扩增和比较研究，力求探究各代菌株的异质性变化程度和范围，了解其产生的原因。结果表明，SNPs在不同单孢代系和家系之间、同一代系和家系内部均稳定存在，但代系间的遗传变异大于家系间的遗传变异，各代系未呈现独特的遗传结构。随着代数的增加，核酸多态性的变化与F1代趋于一致，大部分独特的等位基因型出现在F3代，说明该种COX1基因可能还有大量潜在的新基因型有待发现。重组分析显示，多个SNP位点间发生了频繁的重组事件，说明线粒体异质性在群体内稳定存在，进一步分析发现基因重组和COX1基因多拷贝可能是造成异质性的原因。本研究为进一步揭示COX1基因在捕食线虫丝孢菌进化上的作用，以及评估线粒体基因在该类真菌分子鉴定中的效果提供了科学依据。
关键词(KeyWords)： COX1基因;单核苷酸多态性;单孢菌株;重组
基金项目(Foundation): 国家自然科学基金(31760010)~~
作者(Authors): 李烁烁;王达;房美玲;张颖;
DOI: 10.13346/j.mycosystema.210285
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