应用5.8S rDNA及ITS区序列分析链格孢种级分类APPLICATION OF SEQUENCING OF 5.8S rDNA, ITS1 AND ITS2 ON IDENTIFICATION AND CLASSIFICATION OF ALTERNARIA AT SEPECIES LEVEL
王洪凯,张天宇,张猛
摘要(Abstract)：
对9个链格孢小孢子种和3个大孢子种共20个链格孢菌株的5.8S rDNA及其两侧的ITS1区和ITS2区进行了序列分析。聚类分析结果表明形态差异大的种可以明确加以区分，而供试的9个小孢子种之间差异很小，不能根据对所选区段的序列分析加以区分。传统分类上的滨菊链格孢不属于Alternaria, 其分类地位需进一步研究。
关键词(KeyWords)： 链格孢;序列分析;ITS区;rDNA5.8s段
基金项目(Foundation): 国家自然科学基金资助项目!(项目号：39770003)&&
作者(Author): 王洪凯,张天宇,张猛
参考文献(References)：
- 张敬泽,张天宇,王瑾, 1997. 链格孢属种间培养性状的分类研究.浙江农业大学学报, 23(5):511～514
- AdachiY H,Wantanabe K,1993. Nuclear ribiosomal DNA as a probe for genetic variabality in the japanese pear pathotype Alternaria alternata. Appl Environm Microbiol, 59:3197～32005
- Andersen B,Thrane U, 1996. Differentiation of Alternaria infectoria and Alternaria alternata based on morphology, metabolite profiles, and cultural characteristics. Can J Microbiol, 42:685～689
- Brower A V, Desalle R, Vogler A, 1996. Gene tree, species trees, and systemtics: A Cladistic Perspective. Ann Rev Ecol Syst ,27:423～50
- Bruns T D, White T J, Taylor J W, 1991. Fungal molecular systematics. Annu Rev Ecol Syst, 22: 525～64
- Edwards K, Johnstone C, Thompson C, 1991. A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Research, 19: 1349
- Jasalavich C A, et al., 1995. Comparison of nuclear ribosomal DNA sequences from Alternaria species pathogenic to crucifers.Mycol Res 99(5): 604～614
- Kusaba M, Tsuge T, 1995. Phylogeny of Alternaria fungi known to produce host-specific toxins on the basis of variation in internal transcribed spacers of ribosomal DNA. Current Genetics, 28: 491～498
- Kusaba M, Tsuge T, 1994. Nuclear ribosomal DNA variation and pathogenic specialization in Alternaria fungi known to produce host-specific toxins. Applied and environmental microbiology, 60(9): 3055～3062
- Kuninaga S, Yokosawa R, 1987. Studies on the taxonomy of plant pathogenic fungi by a comparison of DNA homology 1.Genetic relatedness among species in the genus Alternaria. Ann Phytopathol Soc Jpn, 53: 369～369
- Nishimura S, 1983. Host-specific toxins and chemical structures from Alternaria species. Ann Rev Phytopathol, 21: 87～116
- Rehner S A, Samuels G J, 1994. Taxonomy and phylogeny of gliocladium analysised from nuclear large subunit ribosomal DNA sequence. Mycol Res, 98(6): 625～634
- Simmons E G, 1993. Alternaria themes and variations(73) . Mycotaxon, 48: 109～140
- Simmons E G, 1998. Alternaria themes and variations (224-225). Mycotaxon, 68: 417～427
- Simmons E G, 1992. Alternaria taxonomy: current status, viewpoint , challenge. In: Chelkowski J & Visconti A (Eds.) Alternaria: Biology Plant Disease and Metabolites. Amsterdam : Elsevier. 1～29
- Simmons E G, 1999. Alternaria themes and variations (226-243) .Mycotaxon 70: 263～369