陈名君,林俨,黄勃

• 1:安徽农业大学安徽省微生物防治重点实验室

摘要(Abstract)：

根虫瘟霉是最常见的一种虫霉,寄主广泛,世界广布。目前有学者认为该种是个复寄主的根虫瘟霉及其近缘类群总计19个菌株,进行3个靶位点(ITèU合种。S、LSU D本研究对世界不同地区和不同rDNA、RPB2)的分子系统发育学分析。结果显示,根虫瘟霉ITS长度较为保守性,介于1 321–1 324bp之间,而所研究的虫霉亚门的其他类群的长度范围较大,为556–1654bp。本研究确认根虫瘟霉是单系种,同时西虫瘟霉、矛孢虫瘟霉和英吉利虫瘟霉具有明确种的分类地位。鬼笔状虫瘟霉种应该被视为西虫瘟霉的异名。

关键词(KeyWords)： 虫霉门;ITS长度;复合种;系统发育学

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31772226);; 2019年度高校优秀青年人才支持计划重点项目(gxyqZD2019013)~~

作者(Author): 陈名君,林俨,黄勃

DOI: 10.13346/j.mycosystema.190203

参考文献(References)：

• Ba?azy S,1993.Entomophthorales.Flora of Poland(Flora Polska),Fungi(Mycota).Vol.24.Polish Academy of Science,W Szafer Inst Botany,Kraków.1-356
• Batko A,1964.On the new genera:Zoophthora gen.nov.,Triplosporium(Thaxter)gen.nov.,and Entomophaga gen.nov.(Phycomycetes:Entomophthoraceae).Bulletin de l’Academie Polonaise des Sciences,Série des Sciences Biologiques,12:323-326
• Glare TR,Milner RJ,Chilvers GA,Mahon RJ,Brown WV,1987.Taxonomic implications of intraspecific variation amongst isolates of the aphid-pathogenic fungi Zoophthora radicans Brefeld and Z.phalloides Batko(Zygomycetes,Entomophthoraceae).Australian Journal of Botany,35:49-67
• Gryganskyi AP,Humber RA,Miadlikovska J,Smith ME,Wu S,Voigt K,Walther G,Anishchenko IM,Vilgalys R,2012.Molecular phylogeny of the Entomophthoromycota.Molecular Phylogeny and Evolution,65:682-694
• Gryganskyi AP,Humber RA,Smith ME,Hodge K,Huang B,Voigt K,Vilgalys R,2013.Phylogenetic lineages in Entomophthoromycota.Persoonia,30:94-105
• Humber RA,1989.Synopsis of a revised classification for the Entomophthorales(Zygomycotina).Mycotaxon,34:441-460
• Lacey LA,1997.Manual of techniques in insect pathology.UKAcademic Press,Harcourt Brace&Company,London.1-409
• Li ZZ,Fan MZ,Huang B,2000.Flora fungorum sinicorum.Vol.13.Entomophthorales.Science Press,Beijing.1-168(in Chinese)
• Liu M,Rombach MC,Humber RA,Hodge KT,2005.What’s in a name?Aschersonia insperata:a new pleoanamorphic fungus with characteristics of Aschersonia and Hirsutella.Mycologia,97:246-253
• Posada D,Crandall KA,1998.MODELTEST:testing the model of DNA substitution.Bioinformatics,14:817-818
• Rambaut A,2012.FigTree version 1.4.0.Available at http://tree.bio.ed.ac.uk/software/figtree/
• Ronquist F,Huelsenbeck JP,2003.MRBAYES 3:Bayesian phylogenetic inference under mixed models.Bioinformatics,19:1572-1574
• Silvestro D,Michalak I,2012.RaxmlGUI:a graphical front-end for RAxML.Organisms Diversity&Evolution,12:335-337
• Spatafora JW,Chang Y,Benny GL,Lazarus K,Smith ME,Berbee ML,Bonito G,Corradi N,Grigoriev I,Gryganskyi A,James TY,O’Donnell K,Roberson RW,Taylor TN,Uehling J,Vilgalys R,White MM,Stajich JE,2016.Aphylum-level phylogenetic classification of zygomycete fungi based on genomescale data.Mycologia,108(5):1028-1046
• Thompson JD,Higgins DG,Gibson TJ,1994.Clustal W:improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice.Nucleic Acids Research,22:4673-4680
• Vilgalys R,Hester M,1990.Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species.Journal of Bacteriology,172:4238-4246
• White TJ,Bruns T,Lee S,Taylor J,1990.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics.In:Innis MA,Gelfand DH,Sninsky JJ,White TJ(eds.)PCR protocols:a guide to methods and applications.Academic Press,San Diego.315-322
• Yang RH,Su JH,Shang JJ,Wu YY,Li Y,Bao DP,Yao YJ,2018.Evaluation of the ribosomal DNA internal transcribed spacer(ITS),specifically ITS1 and ITS2,for the analysis of fungal diversity by deep sequencing.PLoS One,13(10):https://doi.org/10.1371/journal.pone.0206428
• Zhu H,Qu F,Zhu LH,1994.Isolation of genomic DNAs from fungi using benzyl chloride.Acta Mycological Sinica,13:41-47(in Chinese)
• 李增智，樊美珍，黄勃，2000．中国真菌志.第十三卷（虫霉目）．科学出版社．1-168
• 朱衡，瞿峰，朱立煌，1994．利用氯化苄提取适于分子生物学分析的真菌DNA．真菌学报，13(1):34-40