赵燕珍;冯万艳;冯婧玮;孙学广;

• 1:贵州大学贵州省森林资源与环境研究中心林学院

摘要(Abstract)：

深色有隔内生真菌(dark septate endophyte,DSE)广泛定殖于植物根系,对促进植物生长、提高植物抗逆能力具重要作用。本研究从马尾松Pinusmassoniana根系分离到一株DSE,于无菌条件下研究了此菌与马尾松的共生特征,研究结果表明:基于形态学和分子生物学分析,此菌被鉴定为福廷瓶头霉Phialocephala fortinii,在PDA培养基25℃培养条件下,此菌不产孢,菌丝深色、具隔,最适培养基为PDA。此菌与马尾松根系共生体的形成过程可划分为3个时期,即侵入前期:接种后2d,新生菌丝向马尾松根系定向生长并开始接触根系,但未侵入根系内部;侵入期:接种后4d,菌丝与马尾松根系接触侵入根内并在根系皮层细胞间扩展延伸;形成期:接种后6d,菌丝继续在马尾松根系内扩展并形成富含脂类物质的微菌核。接种福廷瓶头霉显著增加了马尾松生物量的积累(P<0.05),并影响了其根系发育。接种后,马尾松主根生长受限,侧根生长显著提高(P<0.05),侧根长度较未接种处理增加了112.87%。被侵染根系的根毛数量减少,并由大量根外菌丝包裹。以上结果对进一步揭示DSE与宿主的共生机理有一定指导意义。

关键词(KeyWords)： 深色有隔内生真菌;福廷瓶头霉;马尾松;形态特征;共生体形成

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31971572,31500090);; 广西创新驱动发展专项(AA17204087-4);; 贵州省一流学科建设项目(GNYL[2017]007)~~

作者(Authors): 赵燕珍;冯万艳;冯婧玮;孙学广;

DOI: 10.13346/j.mycosystema.200003

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