曾召英,许忠顺,牟丹,许绍欢,杜飞,周家喜,邹晓

• 1:贵州大学生命科学学院生态系真菌资源研究所

摘要(Abstract)：

通过分析蝉棒束孢Isaria cicadae内菌核、菌膜及其生境土壤的真菌群落结构与核心真菌组的生态功能,初步探索蝉棒束孢自然生长过程中内环境真菌与其周围环境中真菌的关系。本研究利用高通量测序技术检测6株采自贵阳大将山和贵阳森林公园的蝉棒束孢及土壤样本,结果表明,蝉棒束孢内菌核共检测到43个真菌属,菌膜检测到58个真菌属,菌际土检测到260个真菌属,且内菌核和菌膜真菌群落结构相似。而两地生境土的真菌群落结构有差异,大将山以棒束孢属Isaria、被孢霉属Mortierella和一分类地位未知属占优势,而贵阳森林公园以被孢霉属、隐球酵母属Cryptococcus、红菇属Russula、棒束孢属占优势,且两地菌际土中棒束孢属的丰度均显著高于其他土样。核心真菌组检测分析显示,内菌核的核心真菌组有10个OTUs,菌膜的核心真菌组有5个OTUs,菌际土的核心真菌组有20个OTUs,3种样本的核心真菌组的生态功能群均检测到有与植物相关的功能群,内菌核中检测到外生菌根真菌群,菌膜中检测到植物病原真菌群,菌际土中检测到丛枝菌根真菌群和植物病原菌群。

关键词(KeyWords)： 蝉棒束孢;内菌核;菌膜;菌际土;核心真菌组;生态功能群

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金（31860037）;; 贵州省科技计划项目(黔科合支撑[2019]2405，黔科合平台人才[2017]5617)~~

作者(Author): 曾召英,许忠顺,牟丹,许绍欢,杜飞,周家喜,邹晓

DOI: 10.13346/j.mycosystema.190138

参考文献(References)：

• Bailey KL,Pitt WM,Leggett F,Sheedy C,Derby J,2011.Determining the infection process of Phoma macrostoma that leads to bioherbicidal activity on broadleaved weeds.Biological Control,59(2):268-276
• Behie SW,Bidochka MJ,2014.Ubiquity of insect-derived nitrogen transfer to plants by endophytic insect-pathogenic fungi:an additional branch of the soil nitrogen cycle.Applied and Environmental Microbiology,80(5):1553-1560
• Behie SW,Moreira CC,Sementchoukova I,Barelli L,Zelisko PM,Bidochka MJ,2017.Carbon translocation from a plant to an insect-pathogenic endophytic fungus.Nature Communications,8:14245
• Behie SW,Zelisko PM,Bidochka MJ,2012.Endophytic insect-parasitic fungi translocate nitrogen directly from insects to plants.Science,336(6088):1576
• Bianca A,Farag MA,Choong-Min R,Jean-Marc G,2015.Role of bacterial volatile compounds in bacterial biology.Fems Microbiology Reviews,39(2):222-233
• Cairney JWG,1992.Translocation of solutes in ectomycorrhizal and saprotrophic rhizomorphs.Mycological Research,96(2):135-141
• Chen FJ,1992.Mortierella species in China.Mycosystema,11(Z1):23-64(in Chinese)
• Chen ZA,Li ZZ,Chen YP,2014.Jin Chanhuan.Tranditional Chinese Medicine Classics Press,Beijing.52-53(in Chinese)
• Dong CB,Zhang ZY,Han YF,Liang ZQ,2019.Research and application prospects of core microbiome.Mycosystema,38(1):1-10(in Chinese)
• Ho S,Jiang Y,Chen F,2007.Polyunsaturated fatty acids(PUFAs)content of the fungus Mortierella alpina isolated from soil.Journal of Agricultural and Food Chemistry,55(10):3960-3966
• Hu SL,Liang JD,Chen WH,Zou X,Han YF,Liang ZQ,2018.Multiple fungi colonized in the endo-sclerotia of Metacordyceps neogunnii.Mycosystema,37(9):1199-1206(in Chinese)
• Huang HJ,2017.Imitating ecological and substitute cultivation of Cordyceps cicadae in bamboo forest.Edible Fungi of China,36(3):90-92(in Chinese)
• Jin GS,2012.Research on molecular detection and fungal communities of soil from the habitats of Ophiocordyceps sinensis.Master Thesis,The University of Chinese Academy of Sciences,Beijing.63-67(in Chinese)
• Jin H,2016.A peliminary research on the quorum-sensing of filamentous Mortierella.Master Thesis,Huazhong University of Science and Technology,Wuhan.48-52(in Chinese)
• Kohlmeier S,Smits TH,Ford RM,2005.Taking the fungal highway:mobilization of pollutant-degrading bacteria by fungi.Environmental Science&Technology,39(12):4640-4646
• Li IC,Lin S,Tsai YT,Hsu JH,Chen YL,Lin WH,Chen CC,2018.Cordyceps cicadae mycelia and its active compound HEAexert beneficial effects on blood glucose in type 2diabetic db/db mice.Journal of the Science of Food&Agriculture,99(2):606-612
• Liang ZQ,Liu AY,Liu ZY,2007.Flora fungorum sinicorum.Vol.32.Cordyceps.Science Press,Beijing.8-9(in Chinese)
• Mao WH,Wu SL,Zhang X,2015.Establish and application of the high throughput sequencing method for soil microbial 16S rDNA using ion torrent PGM.Acta Agriculturae Zhejiangensis,27(12):2165-2170(in Chinese)
• Nguyen NH,Song ZW,Bates ST,Branco S,Tedersoo L,Menke J,Schilling JS,Kennedy PG,2016.FUNGuild:an open annotation tool for parsing fungal community datasets by ecological guild.Fungal Ecology,20:241-248
• Olatunji OJ,Feng Y,Olatunji OO,Tang J,Ouyang Z,Su Z,2016.Cordycepin protects PC12 cells against6-hydroxydopamine induced neurotoxicity via its antioxidant properties.Biomedicine&Pharmacotherapy,81:7-14
• Russo ML,Pelizza SA,Cabello MN,Stenglein SA,Scorsetti AC,2015.Endophytic colonisation of tobacco,corn,wheat and soybeans by the fungal entomopathogen Beauveria bassiana(Ascomycota,Hypocreales).Biocontrol Science and Technology,25(4):475-480
• Sharma SK,Gautam N,Atri NS,2015.Optimized extraction,composition,antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae.BMC Complementary&Alternative Medicine,15(1):446
• Simon A,Bindschedler S,Job D,Wick LY,Filippidou S,Kooli WM,2015.Exploiting the fungal highway:development of a novel tool for the in situ isolation of bacteria migrating along fungal mycelium.FEMS Microbiology Ecology,91(11):66-76
• Tan YQ,Zhang HF,Fan YB,Peng Q,Zhan SF,Chen Y,2014.Diversity of rhizosphere soil fungi of Vitex trifolia.Journal of Fungal Research,12(4):214-217(in Chinese)
• Venturi V,Keel C,2016.Signaling in the rhizosphere.Trends in Plant Science,21(3):187-198
• Wan SN,Ma YP,Wang JW,Wei XY,2014.Identification and antimicrobial activity of endophytic fungi from Pueraria lobata.Natural Product Research and Development,2014(11):1879-1883(in Chinese)
• Wang JH,Zhang ZL,Wang YQ,Yang M,Wang CH,Li XW,Guo YW,2017.Chemical constituents from mycelia and spores of fungus Cordyceps cicadae.Chinese Herbal Medicines,9(2):188-192
• Wang Y,Xu L,Gu YQ,Coleman-Derr D,2016.MetaCoMET:a web platform for discovery and visualization of the core microbiome.Bioinformatics,32(22):3469-3470
• Wang YM,Fan MZ,Li ZZ,2016.Diversity and ecological distribution of endophytic fungi from Taxus chinensis in Huangshan,Anhui.Mycosystema,35(3):360-363(in Chinese)
• Whiteson KL,Simone M,Lim YW,Schmieder R,Maughan H,Quinn R,Blake DR,Conrad D,Rohwer F,2014.Breath gas metabolites and bacterial metagenomes from cystic fibrosis airways indicate active pH neutral2,3-butanedione fermentation.The Isme Journal,8(6):1247-1258
• Xia F,Chen X,Guo MY,Bai XH,Liu Y,Shen GR,Li YL,Lin J,Zhou XW,2016a.High-throughput sequencing-based analysis of endogenetic fungal communities inhabiting the Chinese cordyceps reveals unexpectedly high fungal diversity.Scientific Reports,6:33437
• Xia F,Liu Y,Guo MY,Shen GR,Lin J,Zhou XW,2016b.Pyrosequencing analysis revealed complex endogenetic microorganism community from natural dongchong xiacao and its microhabitat.BMC Microbiology,16(1):196
• Xia F,Liu Y,Shen GR,Guo LX,Zhou XW,2015.Investigation and analysis of microbiological communities in natural Ophiocordyceps sinensis.Canadian Journal of Microbiology,61(2):104-111
• Xu HY,Zhu Y,Cheng DQ,2008.Research progress of traditional Chinese medicine Isaria cicadae.Information on Traditional Chinese Medicine,25(5):27-29(in Chinese)
• Yang NN,Jiang N,Ma QY,Kong FD,Xie QY,Zhou LM,Yu ZF,Zhao YX,2019.Chemical study of the strain Cordyceps spp.from cell fusion between Cordyceps militaris and Cordyceps cicadae.Journal of Asian Natural Products Research,21(5):449-455
• Zeng WB,Chang CX,Li JP,Wang YB,Dai YD,Yu H,2017.Micro-morphological variation pattern of Isaria cicadae.Acta Microbiologica Sinica,57(3):350-362(in Chinese)
• Zeng WB,Yu H,Ge F,Yang JY,Chen ZH,Wang YB,Dai YD,Adams A,2014.Distribution of nucleosides in populations of Cordyceps cicadae.Molecules(Basel,Switzerland),19(5):6123-6141
• Zhang Y,Li XM,Shang Z,Li CS,Wang BG,2013.Chemical constituents of Penicillium sp.MA-37,a fungus derived from rhizospheres of mangrove plant.Marine Sciences,37(2):7-11(in Chinese)
• Zhang YJ,Sun BD,Zhang S,Wang M,Liu XZ,Gong W,2010.Mycobiotal investigation of natural Ophiocordyceps sinensis based on culture-dependent investigation.Mycosystema,29(4):518-527(in Chinese)
• Zhang YJ,Zhang S,Wang M,Bai FY,Liu XZ,2010.High diversity of the fungal community structure in naturally-occurring Ophiocordyceps sinensis.PLoS One,5(12):15570
• Zheng H,Zhang ZY,Han YF,Chen WH,Liang ZQ,2018.Community composition and ecological functional structural analysis of the endophytic fungi in Robinia pseudoacacia.Mycosystema,37(2):256-263(in Chinese)
• Zhou JX,Yu LF,Zhang XM,Lou YF,Zhang LY,Yang Y,Zou X,2018.Analysis of fungal community composition on the stored tobacco surfaces.Mycosystema,37(4):434-443(in Chinese)
• Zhou XW,Li LJ,Tian EW,2013.Advances in research of the artificial cultivation of Ophiocordyceps sinensis in China.Critical Reviews in Biotechnology,3(34):233
• 曾文波，常衬心，李建平，王元兵，代永东，虞泓，2017.蝉棒束孢显微形态变异式样.微生物学报，57(3):350-362
• 陈法军，1992.中国的被孢霉种类.菌物学报，11(Z1):23-64
• 陈祝安，李增智，陈以平，2014.金蝉花.北京：中医古籍出版社.52-53
• 董醇波，张芝元，韩燕峰，梁宗琦，2019.核心微生物组的研究及利用现状.菌物学报，38(1):1-10
• 胡胜兰，梁建东，陈万浩，邹晓，韩燕峰，梁宗琦，2018.新古尼异虫草内菌核的多菌定殖现象.菌物学报，37(9):1199-1206
• 黄涵锦，2017.竹林蝉花的仿生培育和代料栽培.中国食用菌，36(3):90-92
• 金国胜，2012.冬虫夏草的分子检测及其周际土壤真菌多样性研究.中国科学学院硕士论文，北京.63-67
• 金航，2016.丝状真菌被孢霉生长群体感应的初步研究.华中科技大学硕士论文，武汉.48-52
• 梁宗琦，刘爱英，刘作易，2007.中国真菌志.第32卷.虫草属.北京：科学出版社.8-9
• 毛伟华，吴三玲，张旭，2015.土壤微生物16S rDNA的Ion Torrent PGM高通量检测方法构建与应用.浙江农业学报，27(12):2165-2170
• 谭玉琴，张红芳，樊有赋，彭琴，詹寿发，陈晔，2014.单叶蔓荆根际土壤真菌多样性.菌物研究，12(4):214-217
• 万陕宁，麻玉萍，王家稳，魏希颖，2014.葛内生真菌抑菌活性菌株的筛选及鉴定.天然产物研究与开发，2014(11):1879-1883
• 汪友明，樊美珍，李增智，2016.安徽黄山红豆杉内生真菌多样性与生态分布.菌物学报，35(3):360-363
• 许洪义，朱燕，程东庆，2008.中药蝉花的研究进展.中医药信息，25(5):27-29
• 张一，李晓明，尚卓，李春顺，王斌贵，2013.红树林植物木榄根际土壤真菌Penicillium sp.MA-37的化学成分研究.海洋科学，37(2):7-11
• 张永杰，孙炳达，张姝，旺姆，刘杏忠，巩文，2010.分离自冬虫夏草可培养真菌的多样性研究.菌物学报，29(4):518-527
• 郑欢，张芝元，韩燕峰，陈万浩，梁宗琦，2018.刺槐内生真菌群落组成及其生态功能结构分析.菌物学报，37(2):256-263
• 周家喜，喻理飞，张晓敏，娄元菲，张力元，杨洋，邹晓，2018.仓储烟叶表面真菌群落组成分析.菌物学报，37(4):434-443